Macro-aspartate aminotransferase in a healthy woman

Lorubbio, Maria; Ognibene, Agostino; Salvadori, Barbara; Fanelli, Alessandra

doi:10.3350/cmh.2020.0069

Cited by 4 publications

(5 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The human body synthesizes a variety of amino transferases, with AST being predominantly localized in the mitochondrial cytoplasm of hepatocytes. Clinically, elevated serum AST levels can signify several conditions, encompassing viral hepatitis, alcoholic liver disease, cirrhosis, cholestatic syndrome, acute myocardial infarction, or skeletal muscle damage [ 26 ]. Recent investigations have uncovered a correlation between ALT/AST ratios and insulin resistance, particularly in early to middle-aged women [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Elucidating the role of liver enzymes as markers and regulators in ovarian cancer: a synergistic approach using Mendelian randomization, single-cell analysis, and clinical evidence

Zhu,

Jiang,

et al. 2024

Hum Genomics

View full text Add to dashboard Cite

Objective To investigate the association between liver enzymes and ovarian cancer (OC), and to validate their potential as biomarkers and their mechanisms in OC. Methods Genome-wide association studies for OC and levels of enzymes such as Alkaline phosphatase (ALP), Aspartate aminotransferase (AST), Alanine aminotransferase, and gamma-glutamyltransferase were analyzed. Univariate and multivariate Mendelian randomization (MR), complemented by the Steiger test, identified enzymes with a potential causal relationship to OC. Single-cell transcriptomics from the GSE130000 dataset pinpointed pivotal cellular clusters, enabling further examination of enzyme-encoding gene expression. Transcription factors (TFs) governing these genes were predicted to construct TF-mRNA networks. Additionally, liver enzyme levels were retrospectively analyzed in healthy individuals and OC patients, alongside the evaluation of correlations with cancer antigen 125 (CA125) and Human Epididymis Protein 4 (HE4). Results A total of 283 single nucleotide polymorphisms (SNPs) and 209 SNPs related to ALP and AST, respectively. Using the inverse-variance weighted method, univariate MR (UVMR) analysis revealed that ALP (P = 0.050, OR = 0.938) and AST (P = 0.017, OR = 0.906) were inversely associated with OC risk, suggesting their roles as protective factors. Multivariate MR (MVMR) confirmed the causal effect of ALP (P = 0.005, OR = 0.938) on OC without reverse causality. Key cellular clusters including T cells, ovarian cells, endothelial cells, macrophages, cancer-associated fibroblasts (CAFs), and epithelial cells were identified, with epithelial cells showing high expression of genes encoding AST and ALP. Notably, TFs such as TCE4 were implicated in the regulation of GOT2 and ALPL genes. OC patient samples exhibited decreased ALP levels in both blood and tumor tissues, with a negative correlation between ALP and CA125 levels observed. Conclusion This study has established a causal link between AST and ALP with OC, identifying them as protective factors. The increased expression of the genes encoding these enzymes in epithelial cells provides a theoretical basis for developing novel disease markers and targeted therapies for OC.

show abstract

Section: Discussionmentioning

confidence: 99%

Elucidating the role of liver enzymes as markers and regulators in ovarian cancer: a synergistic approach using Mendelian randomization, single-cell analysis, and clinical evidence

Zhu,

Jiang,

et al. 2024

Hum Genomics

View full text Add to dashboard Cite

show abstract

“…Macro‐AST is a high molecular weight protein complex formed by AST binding with immunoglobulins, lipids or self‐polymerization 12,13 . Other macro‐enzymes with higher prevalence include CPK, lactate dehydrogenase, prolactin and amylase 3,5 . These macro‐enzymes have low renal clearance due to their high molecular weight and therefore elongated half‐life within the plasma 3 …”

Section: Discussionmentioning

confidence: 99%

“…AST elevation may also be associated with macro‐AST (m‐AST)—a high molecular weight protein complex formed in most cases by AST binding with immunoglobulins that is considered as a benign condition 2–6 …”

Section: Introductionmentioning

confidence: 99%

The significance of isolated aspartate aminotransferase elevation in healthy paediatric patients

et al. 2021

View full text Add to dashboard Cite

Aim Aspartate aminotransferase (AST) is an enzyme expressed in several organs; therefore, AST elevation may reflect outside of liver pathology. AST elevation may also be associated with macro‐AST (m‐AST). The aim of this study was to evaluate the long‐term course of children with prolonged isolated AST elevation and the prevalence of m‐AST in our cohort. Methods We retrospectively reviewed the medical charts of children diagnosed with prolonged isolated AST elevation and were evaluated for m‐AST. Results Thirty‐two patients were included. AST elevation persisted for a median of 66.6 months and ranged from 1.23 to 12‐fold upper limit of normal (ULN). Twenty‐two percent were m‐AST positive and 44% had borderline levels of m‐AST. A statistically significant difference was found for age at presentation between the borderline and the positive m‐AST groups (31 vs. 69 months, respectively. p = 0.045). None of the patients with elevated AST developed significant liver disease. Conclusion We confirm the benign course of prolonged isolated AST elevation in general and m‐AST in particular. A fifth of the patients with isolated AST elevation were m‐AST positive. No differences have been found in AST levels between negative, borderline or positive m‐AST.

show abstract

“…4). Можно предположить, что это является проявлением феномена «макроэнзимов», в частности -макро-АсАТ, описанного в ряде клинических случаев [22][23][24][25][26]. В настоящее время не существует адекватного объяснения данного феномена, однако авторы отмечают, что он не связан ни с заболеваниями печени, ни с заболеваниями сердца.…”

Section: печеночные трансаминазыunclassified

Reduction of hepatotoxicity of nimesulide in mechanochemically obtained composition with disodium salt of glycyrrhizic acid

Petrova

Жукова

Evseenko

et al. 2023

Sibirskij nauchnyj medicinskij zhurnal

View full text Add to dashboard Cite

Nimesulide (NIM) is a nonsteroid anti-inflammatory drug which acts as a selective cyclooxygenase 2 inhibitor and is widely used for acute pain treatment. In medical practice, a large amount of data has been collected describing the effect of NIM on the body, while a hepatotoxic side effect of the drug has been found. The exact mechanisms of such NIM-induced hepatotoxicity largely remain unknown but likely involve the intermediate reaction of its metabolism. Reduction of the hepatotoxic side effect of NIM is an actual problem for pharmacology. The aim of the present research was to evaluate the hepatotoxicity of the mechanochemically obtained composition of NIM with glycyrrhizic acid disodium salt (Na2GA) compared to pure NIM and a physical mixture of NIM with Na2GA. Material and methods. CD-1 mice were orally administered for 14 days: 1 group – mechanochemical composition NIM/Na2GA (1:10, m/m) at a dose of 1650 mg/kg; 2 group – physical mixture of NIM with Na2GA (1:10, m/m) at a dose of 1650 mg/kg; 3 group – pure NIM at a dose of 600 mg/kg (which pharmacokinetically corresponds to 1650 mg/kg of NIM/Na2GA); 4 group – vehicle (distilled water). The liver damage was assessed using histological studies and enzymatic activity of the alanine aminotransferase and aspartate aminotransferase in blood serum. Results. Histological analysis did not detect any changes in the liver of NIM/Na2GA-treated animals in comparison with a water-treated group. On the opposite, NIM given alone or as a physical mixture with Na2GA induced severe hepatotoxicity in experimental mice. Biochemical analysis of the blood serum revealed that mechanochemical NIM/Na2GA composition significantly reduced activity of the alanine aminotransferase (about 1.5 times) and aspartate aminotransferase (1.3 times) as compared with the pure NIM. Conclusions. The results obtained indicate a high potential for the practical application of the NIM/Na2GA mechanochemical composition.

show abstract

Macro-aspartate aminotransferase in a healthy woman

Cited by 4 publications

References 15 publications

Elucidating the role of liver enzymes as markers and regulators in ovarian cancer: a synergistic approach using Mendelian randomization, single-cell analysis, and clinical evidence

Elucidating the role of liver enzymes as markers and regulators in ovarian cancer: a synergistic approach using Mendelian randomization, single-cell analysis, and clinical evidence

The significance of isolated aspartate aminotransferase elevation in healthy paediatric patients

Reduction of hepatotoxicity of nimesulide in mechanochemically obtained composition with disodium salt of glycyrrhizic acid

Contact Info

Product

Resources

About