Macromol. Biosci. 11/2017

Rother, Sandra; Galiazzo, Vanessa D.; Kilian, David; Fiebig, Karen M.; Becher, Jana; Moeller, Stephanie; Hempel, Ute; Schnabelrauch, Matthias; Waltenberger, Johannes; Scharnweber, Dieter; Hintze, Vera

doi:10.1002/mabi.201770044

Cited by 7 publications

(8 citation statements)

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“…Aldehyde‐modified lubricin displays enhanced binding to PRG4‐depleted articular cartilage and has been used for cartilage resurfacing and is a convenient method for overcoming loss of lubrication during the early stages of OA . Collagen fibrils have also been embedded in a network of photo‐cross‐linked acrylated HA, CS, or sulfated HA (sHA) 3D hydrogels . Endothelial cell proliferation was significantly increased in sHA gels compared to CS‐derivatized or non‐derivatized HA hydrogels.…”

Section: Application Of Proteoglycans In Cartilage Tissue Engineeringmentioning

confidence: 99%

Glycosaminoglycan and Proteoglycan Biotherapeutics in Articular Cartilage Protection and Repair Strategies: Novel Approaches to Visco‐supplementation in Orthobiologics

Hayes

Melrose

2019

Advanced Therapeutics

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The aim of this study is to review developments in glycosaminoglycan and proteoglycan research relevant to cartilage repair biology and in particular the treatment of osteoarthritis (OA). Glycosaminoglycans decorate a diverse range of extracellular matrix and cell associated proteoglycans conveying structural organization and physico-chemical properties to tissues. They play key roles mediating cellular interactions with bioactive growth factors, cytokines, and morphogenetic proteins, and structural fibrillar collagens, cell interactive and extracellular matrix proteoglycans, and glycoproteins which define tissue function. Proteoglycan degradation detrimentally affects tissue functional properties. Therapeutic strategies have been developed to counter these degenerative changes. Neo-proteoglycans prepared from chondroitin sulfate or hyaluronan and hyaluronan or collagen-binding peptides emulate the interactive, water imbibing, weight bearing, and surface lubricative properties of native proteoglycans. Many neo-proteoglycans outperform native proteoglycans in terms of water imbibition, matrix stabilization, and resistance to proteolytic degradation. The biospecificity of recombinant proteoglycans however, provides precise attachment to native target molecules. Visco-supplements augmented with growth factors/therapeutic cells, hyaluronan, and lubricin (orthobiologicals) have the capacity to lubricate and protect cartilage, control inflammation, and promote cartilage repair and regeneration of early cartilage lesions and may represent a more effective therapeutic approach to the treatment of mild to moderate OA and deserve further study.Similar protective perineural net structures assembled from the lectican proteoglycan family and HA also occur in the CNS/PNS. These so called perineuronal nets are visualized by immunolocalization of MAb 1B5 (+) epitope in rat brain (e) and pericellular 1B5(+) epitope expression by isolated neurons (f). Scale bars 100 µm.

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Section: Application Of Proteoglycans In Cartilage Tissue Engineeringmentioning

confidence: 99%

Glycosaminoglycan and Proteoglycan Biotherapeutics in Articular Cartilage Protection and Repair Strategies: Novel Approaches to Visco‐supplementation in Orthobiologics

Hayes

Melrose

2019

Advanced Therapeutics

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“…For gels with sGAG derivatives, a retarded release of lysozyme and VEGF-A was detected indicating that sGAGs are able to control the interaction with mediator proteins. In addition, sHA-containing gels with or without VEGF-A strongly stimulate the endothelial cell proliferation compared to gels with chondroitin sulfate (CS) or HA alone [3]. Thus, HA/collagen hydrogels as biomimetic materials are able to directly influence endothelial cells in vitro, which might translate into an improved healing of injured vascularized tissues.…”

Section: Resultsmentioning

confidence: 99%

Session 6: Regenerative and Bioinspired Material I

2017

Biomedical Engineering / Biomedizinische Technik

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Direct stem cell encapsulation and cardiac differentiation within supporting biomaterial scaffolds are critical for reproducible and scalable production of the functional human tissues needed in regenerative medicine and drug-testing applications. Producing cardiac tissues directly from pluripotent stem cells rather than assembling tissues using pre-differentiated cells can eliminate multiple cell-handling steps that otherwise limit the potential for process automation and production scale-up. Previously having produced 3D cardiac tissues directly from human pluripotent stem cells (hPSCs) through hiPSC encapsulation and direct differentiation in PEG-fibrinogen (PEG-Fb), this study established a rapid, one-step hiPSC microsphere encapsulation system and used it to directly produce functional cardiac tissue microspheres in suspension culture.Following suspension of hiPSCs in PEG-Fb precursor solution, microspheres were rapidly formed (<1 sec) within a custom built microfluidic device through emulsification and photo-crosslinked using a full spectrum visible light source (day -3). Cell-laden microspheres were maintained three days in mTeSR-1 media and then differentiation was initiated (day 0). Microspheres were highly uniform and encapsulated hiPSCs remained viable and continued to divide, forming dense cell-laden spheroids. Following initiation of differentiation (day 0), microsphere diameter continued increasing as a result of cell growth. First isolated areas of contraction were typically seen on day 8 of differentiation, with microspheres establishing synchronous tissue-level contraction by day 14 and retaining their function for several months. Microspheres supported high efficiency cardiac differentiation with 71.6±8.4% cTnT+, 7.1±1.7% cTnT+/Ki67+, and 8.4±6.5% P4HB+ cells (day 20, n=3 separate batches). Microsphere cardiomyocytes (CMs) could be exogenously paced up to 6.0 Hz and responded appropriately to isoproterenol and propranolol. CMs stained positive for αSA with aligned and well-defined sarcomeres.In summary, rapid encapsulation of hiPSCs in PEG-Fb microspheres supported high-efficiency, direct differentiation to obtain 3D cardiac microspheres. Direct cardiac tissue formation through hPSC encapsulation in PEG-Fb provides the ability to guide cardiac differentiation, to produce functional CMs, and to obtain highly reproducible cell-laden microspheres in scalable suspension culture. Abstracts -Annual Meeting of the German Society for Biomaterials 2017 -Würzburg, November 09 -11 • DOI 10.1515/bmt-2017-6006 Biomed. Eng. -Biomed. Tech. 2017; 62(s2): p34-p39 • © by Walter de Gruyter • Berlin •

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“…The corresponding HA acrylate could be synthesized with acryolyl chloride both as mentioned above in a dichloromethane/water mixture (Qin et al, 2014), and with addition of tetra-n-butylammonium fluoride as phasetransfer catalyst (Becher et al, 2013). In analogy to nonderivatized HA, the sulfated derivatives with different degree of sulfation (DS: 0.9-1.4) can be (meth)acrylated in the same manner (Rother et al, 2017a). Further, HA esters of unsaturated carboxylic acids as pentenoates (Mergy et al, 2012) and maleates (Vasi et al, 2014) are available by treating HA with the corresponding acid anhydrides (see for example Smejkalova et al, 2014).…”

Section: Esterification Reactionsmentioning

confidence: 99%

“…13 C-NMR confirmed a complete sulfation of the secondary hydroxyl groups and a free primary hydroxyl group. A subsequent esterification of the differently sulfated HA, for example, with (meth)acrylate groups result in mixed HA derivatives bearing both sulfate and (meth)acrylate units in different positions of the disaccharide repeating unit (Rother et al, 2017a;Becher et al, 2012, Figure 7). The introduction of a phosphate group into HA could be attractive for their application to biochemical systems via mediation of GAG-protein interactions.…”

Section: Sulfate and Phosphate Estersmentioning

confidence: 99%

Chemical Modification of Hyaluronan and Their Biomedical Applications

2022

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Hyaluronan, the extracellular matrix glycosaminoglycan, is an important structural component of many tissues playing a critical role in a variety of biological contexts. This makes hyaluronan, which can be biotechnologically produced in large scale, an attractive starting polymer for chemical modifications. This review provides a broad overview of different synthesis strategies used for modulating the biological as well as material properties of this polysaccharide. We discuss current advances and challenges of derivatization reactions targeting the primary and secondary hydroxyl groups or carboxylic acid groups and the N-acetyl groups after deamidation. In addition, we give examples for approaches using hyaluronan as biomedical polymer matrix and consequences of chemical modifications on the interaction of hyaluronan with cells via receptor-mediated signaling. Collectively, hyaluronan derivatives play a significant role in biomedical research and applications indicating the great promise for future innovative therapies.

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Macromol. Biosci. 11/2017

Cited by 7 publications

References 0 publications

Glycosaminoglycan and Proteoglycan Biotherapeutics in Articular Cartilage Protection and Repair Strategies: Novel Approaches to Visco‐supplementation in Orthobiologics

Glycosaminoglycan and Proteoglycan Biotherapeutics in Articular Cartilage Protection and Repair Strategies: Novel Approaches to Visco‐supplementation in Orthobiologics

Session 6: Regenerative and Bioinspired Material I

Chemical Modification of Hyaluronan and Their Biomedical Applications

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