Abstract:Back Cover: The double emulsion capsules (DECs) can improve the accumulation of drug‐incorporated targeted‐supermolecules at targeted tumor site through combined passive and positive targeting mediated by high frequency magnetic field (HFMF) stimulus. Dual targeted thermal‐chemotherapy of supermolecules involves an internalization of large amounts of drugs to cancer cell, which is actuated by cancer‐cell binding proteins on supermolecules, all contributing to cell kill. Further details can be found in the arti… Show more
“…Gene expression level was assessed by quantitative real-time polymerase chain reaction (PCR) on an Applied Biosystems 7500 fast realtime PCR machine and analyzed following a published protocol. 24 In short, samples were run at 95 8C for 30 s, followed by 45 cycles of 95 8C for 5 s, 55 8C for 30 s, and at 72 8C for 30 s. Within each experimental group, the detected levels of the genes of interest were normalized to the level of house-keeping gene GAPDH. Gene expression levels were calculated using 2 2DDCT method where the expression level of 2D culture was set as onefold after having normalized all other groups to the 2D undifferentiated groups for each respective day.…”
Section: Characterizations Of Cell Viability and Functionmentioning
“…Gene expression level was assessed by quantitative real-time polymerase chain reaction (PCR) on an Applied Biosystems 7500 fast realtime PCR machine and analyzed following a published protocol. 24 In short, samples were run at 95 8C for 30 s, followed by 45 cycles of 95 8C for 5 s, 55 8C for 30 s, and at 72 8C for 30 s. Within each experimental group, the detected levels of the genes of interest were normalized to the level of house-keeping gene GAPDH. Gene expression levels were calculated using 2 2DDCT method where the expression level of 2D culture was set as onefold after having normalized all other groups to the 2D undifferentiated groups for each respective day.…”
Section: Characterizations Of Cell Viability and Functionmentioning
This review systematically summarizes the research status, challenges, prospects, and potential bench-to-bedside translation of minimally invasive nanomedicines.
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