Macromolecular diffusion and release from self-assembled β-hairpin peptide hydrogels

Branco, Monica C.; Pochan, Darrin J.; Wagner, Norman J.; Schneider, Joel P.

doi:10.1016/j.biomaterials.2008.11.019

Cited by 221 publications

(233 citation statements)

References 48 publications

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“…Earlier reports suggest FRAP as a convenient and reliable method to understand the self-diffusion coefficient in hydrogels and also the matrix-probe interaction during the course of diffusion. [18] For this, fluorescent probe FITC and FITC-BSA were homogeneously mixed with the hydrogel A8 and a high-intensity laser was used to photobleach a particular area in the hydrogel of radius 8 µm ( Figure 3C). After bleaching, the laser power was attenuated and the recovery of fluorescence was captured.…”

Section: Study Of Diffusion Of Small Molecules and Proteins From The mentioning

confidence: 99%

Controlled Exposure of Bioactive Growth Factor in 3D Amyloid Hydrogel for Stem Cells Differentiation

Das

Kumar

Jha

et al. 2017

Adv Healthcare Materials

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promoting regeneration. [3] Hydrogels present a unique microstructure to the encapsulated cells influencing cell signaling and hence cellular fate. Peptide hydrogels are a class of physical hydrogels where the component peptides self-assemble noncovalently to form an ordered nanostructure [4] and eventually supramolecular networks, which entrap water for gelation. [5] Recently with the advent of protein engineering, relatively simple peptide based hydrogels have gained increasing attention in biomaterial development. [6] The major advantage of employing short peptides as hydrogelators is that their properties could be easily tuned and controlled via side chain modification and chemical alteration to achieve the desired functionality. [7] Moreover, peptide hydrogels are also advantageous as they have less potential for inflammation and also have biodegradable components [8] compared to polymer based hydrogels. Recently, we have shown the application of another class of hydrogels based on "amyloid fibrils" for possible application in 2D, 3D cell culture, and stem cell differentiation. [2a,b] Amyloids are highly stable protein/peptide aggregates with cross-β-sheet rich structure. [9] Although amyloids are historically associated with various human diseases, recent discoveries have suggested functional roles of amyloids that help organisms survival rather causing diseases. [10] Also, studies have shown that amyloid fibrils are rather benign or less toxic species compared to soluble oligomers. [11] Due to higher order structure, amyloid possesses unique stability against various harsh environmental conditions such as wide ranges of pHs, temperature, and proteases, which makes amyloids attractive for designing biomaterials for tissue engineering as well as various nanotechnological applications. [2h,12] Recently, a number of studies also indicate that amyloids are unique cell adhesive material that promotes adhesion of cells without the presence of tripeptide motif Arg-Gly-Asp (RGD) motif. [13] Our recent study has also shown that amyloid based hydrogels could be suitably used as implantable materials for tissue engineering, owing to their noninflammatory property along with ease of delivery with minimally invasive surgery. [2b] In a natural tissue environment, extra cellular matrix (ECM) has a favorable nanotopography [14] for cells and contains large depots of growth factors that affect the physiological functions of the cells. [3c] In an attempt to mimic such a natural niche to provide better stem cell differentiation, presence and controlled exposure of various growth factors are Amyloid based hydrogels can mimic the extracellular matrix and serve as matrices for tissue engineering both in vitro and in vivo. A pH responsive selfassembled amyloid hydrogel system is used to encapsulate various growth factors for driving stem cell differentiation toward neuronal lineage. Diffusion studies with fluorescence recovery after photobleaching and bulk release with the model protein fluorescein isothiocyanate-bovin...

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Section: Study Of Diffusion Of Small Molecules and Proteins From The mentioning

confidence: 99%

Controlled Exposure of Bioactive Growth Factor in 3D Amyloid Hydrogel for Stem Cells Differentiation

Das

Kumar

Jha

et al. 2017

Adv Healthcare Materials

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“…In particular, peptide-based self-assemblies have drawn much attention because of their unique properties including well biocompatibility, chemical versatility, and biological recognition abilities. [1][2][3][4] To date, self-assembled peptide-based materials have become a class of very important biocompatible materials that have found various applications in tissue engineering, [5][6][7] drug delivery, [8][9][10] wound healing, [ 11 , 12 ] and inhibitor screening. [ 13 , 14 ] The self-assembly of peptide can be generally divided into two categories: spontaneous self-assembly and induced self-assembly.…”

Section: Introductionmentioning

confidence: 99%

Biological Glucose Metabolism Regulated Peptide Self‐Assembly as a Simple Visual Biosensor for Glucose Detection

Lin

Feng

et al. 2012

Macromol. Rapid Commun.

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A glucose oxidase (GOx)-mediated glucose metabolism was in vitro mimicked and employed to regulate the self-assembly of peptide-based building blocks. In this new stimuli-responsive self-assembly system, two peptide-based building blocks, respectively, having aspartic acid (gelator 1) and lysine (gelator 2) residues were designed and prepared. When adding glucose and GOx to the aqueous solution of gelator 1 or the self-assembled fibrillar hydrogel of gelator 2 to construct glucose metabolism system, the metabolic product (gluconic acid) can trigger the protonation of the peptide molecules and induce the phase transitions of gelators 1 (sol-gel) and 2 (gel-sol). Because this glucose metabolism regulated peptide self-assembly is built on the oxidation of glucose, it can be used as a simple visual biosensor for glucose detection.

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“…[20][21][22][23][24] Benefits have been demonstrated for the use of these peptide hydrogels in cartilage, 25,26 liver, 27 and cardiovascular 28,29 tissues, and they have been shown to provide a microenvironment that enhances the chondrogenesis of BMSCs. 26,30 These peptides can also control the delivery and release of functional proteins, 21 therapeutic macromolecules, 22 and bioactive motifs. 20 Taken together, these studies suggest the potential to co-encapsulate growth factors and BMSCs in self-assembling peptide hydrogels to generate cartilage neotissue.…”

mentioning

confidence: 99%

Controlled Delivery of Transforming Growth Factor β1 by Self-Assembling Peptide Hydrogels Induces Chondrogenesis of Bone Marrow Stromal Cells and Modulates Smad2/3 Signaling

Kopesky

Vanderploeg²,

Kisiday

et al. 2011

Tissue Engineering Part A

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Self-assembling peptide hydrogels were modified to deliver transforming growth factor b1 (TGF-b1) to encapsulated bone-marrow-derived stromal cells (BMSCs) for cartilage tissue engineering applications using two different approaches: (i) biotin-streptavidin tethering; (ii) adsorption to the peptide scaffold. Initial studies to determine the duration of TGF-b1 medium supplementation necessary to stimulate chondrogenesis showed that 4 days of transient soluble TGF-b1 to newborn bovine BMSCs resulted in 10-fold higher proteoglycan accumulation than TGF-b1-free culture after 3 weeks. Subsequently, BMSC-seeded peptide hydrogels with either tethered TGF-b1 (Teth-TGF) or adsorbed TGF-b1 (Ads-TGF) were cultured in the TGF-b1-free medium, and chondrogenesis was compared to that for BMSCs encapsulated in unmodified peptide hydrogels, both with and without soluble TGF-b1 medium supplementation. Ads-TGF peptide hydrogels stimulated chondrogenesis of BMSCs as demonstrated by cell proliferation and cartilage-like extracellular matrix accumulation, whereas Teth-TGF did not stimulate chondrogenesis. In parallel experiments, TGF-b1 adsorbed to agarose hydrogels stimulated comparable chondrogenesis. Full-length aggrecan was produced by BMSCs in response to Ads-TGF in both peptide and agarose hydrogels, whereas medium-delivered TGF-b1 stimulated catabolic aggrecan cleavage product formation in agarose but not peptide scaffolds. Smad2/3 was transiently phosphorylated in response to Ads-TGF but not Teth-TGF, whereas medium-delivered TGF-b1 produced sustained signaling, suggesting that dose and signal duration are potentially important for minimizing aggrecan cleavage product formation. Robustness of this technology for use in multiple species and ages was demonstrated by effective chondrogenic stimulation of adult equine BMSCs, an important translational model used before the initiation of human clinical studies.

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Macromolecular diffusion and release from self-assembled β-hairpin peptide hydrogels

Cited by 221 publications

References 48 publications

Controlled Exposure of Bioactive Growth Factor in 3D Amyloid Hydrogel for Stem Cells Differentiation

Controlled Exposure of Bioactive Growth Factor in 3D Amyloid Hydrogel for Stem Cells Differentiation

Biological Glucose Metabolism Regulated Peptide Self‐Assembly as a Simple Visual Biosensor for Glucose Detection

Controlled Delivery of Transforming Growth Factor β1 by Self-Assembling Peptide Hydrogels Induces Chondrogenesis of Bone Marrow Stromal Cells and Modulates Smad2/3 Signaling

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