Macroscopics upramolecular assembly(MSA) is an ew concept in supramolecular science with af ocus on interfacial assembly of macroscopic building blocks, which has largely extended the applicable materials of supramolecular assembly and provided new solutions to fabricating tissue scaffolds, soft devices,e tc. The precision of the assembled structures is of great interest;unlike molecular assemblies, MSA precision is highly dependento nt he matching degree of assembled surfaces because of the large interactive area and group number,w hich result in remarkablyi ncreasedk inetic possibilities and metastable assemblies.T his Concept introduces the principle, history, and development of MSA, elaborates the low-precision challenge in MSA, summarizes the strategies for precise MSA based on the different thermodynamic stabilityo f precise/imprecise structures and control over assembly kinetics, and finally demonstrates the applicationso fp recise MSA structures in advanced manufacture such as tissue scaffolds.