Magnaporthe oryzae cell wall hydrolysate induces ROS and fungistatic VOCs in rice cell cultures

Forlani, Giuseppe; Occhipinti, Andrea; Bossi, Simone; Bertea, Cinzia M.; Varese, Cristina; Maffei, Massimo E.

doi:10.1016/j.jplph.2011.06.014

Cited by 8 publications

(5 citation statements)

References 41 publications

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“…The role of 1-octanol in flowering panicles may be associated with its antifungal and antibacterial activity (Rodov and Nafussi 2011 ). 1-Octanol has been observed to induce ROS production in elicited and non-elicited rice cells and showed fungistatic activity against the pathogen Magnaporthe oryzae (Forlani et al 2011 ). Thus maximum accumulation of 1-octanol in flowering panicles might protect the flowers and developing embryos from biotic stress.…”

Section: Resultsmentioning

confidence: 99%

Aroma volatile analyses and 2AP characterization at various developmental stages in Basmati and Non-Basmati scented rice (Oryza sativa L.) cultivars

Hinge

Patil

Nadaf

2016

Rice

126

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BackgroundRice plant growth is comprised of distinct phases, such as vegetative, reproductive, grain filling and maturity phases. In these phases synthesis and availability of primary and secondary metabolites including volatile organic compounds (VOC’s) is highly variable. In scented rice, aroma volatiles are synthesized in aerial plant parts and deposited in mature grains. There are more than 100 VOCs reported to be responsible for flavor in basmati rice. It will be interesting to keep track of aroma volatiles across the developmental stages in scented rice. Therefore, the aroma volatiles contributing in aroma with special reference to the major compound 2 acetyl-1-pyrroline (2AP) were screened at seven developmental stages in scented rice cultivars Basmati-370 and Ambemohar-157 along with non-scented rice cultivar IR-64 as a control following HS-SPME-GC-MS method. In addition, the expression levels of key genes and precursor levels involved in 2AP biosynthesis were studied.ResultsThe study indicated that volatilome of scented rice cultivars is more complex than non-scented rice cultivar. N-heterocyclic class was the major distinguishing class between scented from non-scented rice. A total of 14 compounds including, 2AP were detected specifically in scented rice cultivars. Maximum number of compounds were synthesized at seedling stage and decreased gradually at reproductive and maturity. The seedling stage is an active phase of development where maximum number green leaf volatiles were synthesized which are known to act as defense molecules for protection of young plant parts. Among the 14 odor active compounds (OACs), 10 OACs were accumulated at higher concentrations significantly in scented rice cultivars and contribute in the aroma. 2AP content was highest in mature grains followed by at booting stage. Gene expression analysis revealed that reduced expression of betaine aldehyde dehydrogenase 2 (badh2) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and elevated level of triose phosphate isomerase (TPI) and Δ1-Pyrolline-5-carboxylic acid synthetase (P5CS) transcript enhances 2AP accumulation.ConclusionsMost diverse compounds were synthesized at seedling stage and OACs were accumulated more at flowering followed by seedling stage. Distinct accumulation pattern exists for 2AP and other aroma volatiles at various developmental stages. The study revealed the mechanism of 2AP accumulation such that 2AP in mature grains might be transported from leaves and stem sheath and accumulation takes place in grains.Electronic supplementary materialThe online version of this article (doi:10.1186/s12284-016-0113-6) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Aroma volatile analyses and 2AP characterization at various developmental stages in Basmati and Non-Basmati scented rice (Oryza sativa L.) cultivars

Hinge

Patil

Nadaf

2016

Rice

126

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“…The remaining 53 peaks were relatively evenly distributed across the five microbial samples (Table 2, Supplementary Table 2). Based upon mass spectral and chromatographic data, we were able to assign putative identities to eight VOCs, which included known bioactive compounds such as dimethyl disulfide, dimethyl trisulfide, indole, 1-octanol, and octanoic acid (Kai et al, 2009;Schulz et al, 2010;Forlani et al, 2011;Groenhagen et al, 2013;Giorgio et al, 2015;Lo Cantore et al, 2015). For the unnamed compounds, we were able to assign chemical classifications to 30 of them.…”

Section: Characterizing the Vocs Of C Vaccinii And Phoma Sp By Sbse-gc×gc-tofmsmentioning

confidence: 99%

Inhibition of Fungal Growth and Induction of a Novel Volatilome in Response to Chromobacterium vaccinii Volatile Organic Compounds

et al. 2020

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The study of chemical bioactivity in the rhizosphere has recently broadened to include microbial metabolites, and their roles in niche construction and competition via growth promotion, growth inhibition, and toxicity. Several prior studies have identified bacteria that produce volatile organic compounds (VOCs) with antifungal activities, indicating their potential use as biocontrol organisms to suppress phytopathogenic fungi and reduce agricultural losses. We sought to expand the roster of soil bacteria with known antifungal VOCs by testing bacterial isolates from wild and cultivated cranberry bog soils for VOCs that inhibit the growth of four common fungal and oomycete plant pathogens, and Trichoderma sp. Twenty one of the screened isolates inhibited the growth of at least one fungus by the production of VOCs, and isolates of Chromobacterium vaccinii had broad antifungal VOC activity, with growth inhibition over 90% for some fungi. Fungi exposed to C. vaccinii VOCs had extensive morphological abnormalities such as swollen hyphal cells, vacuolar depositions, and cell wall alterations. Quorum-insensitive cviR − mutants of C. vaccinii were significantly less fungistatic, indicating a role for quorum regulation in the production of antifungal VOCs. We collected and characterized VOCs from co-cultivation assays of Phoma sp. exposed to wild-type C. vaccinii MWU328, and its cviR − mutant using stir bar sorptive extraction and comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (SBSE-GC×GC-TOFMS). We detected 53 VOCs that differ significantly in abundance between microbial cultures and media controls, including four candidate quorum-regulated fungistatic VOCs produced by C. vaccinii. Importantly, the metabolomes of the bacterial-fungal co-cultures were not the sum of the monoculture VOCs, an emergent property of their VOC-mediated interactions. These data suggest semiochemical feedback loops between microbes that have co-evolved for sensing and responding to exogenous VOCs.

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“…Six of these compounds (5,5 dibutylnonane, 3-ethyl-2-heptanol, 2-hexanol, 2-nonanol, 3-nonanol, and trans-3-dodecene) have been found in other species [49][50][51][52][53]. Interestingly, trans-3-dodecene was found in rice cells elicited by rice blast (Magnaporthe oryzae) [54]. The compound 2-hexanol, known to elicit responses in several insects [55,56], has been recently found in grape berries [57].…”

Section: Discussionmentioning

confidence: 99%

Characterization of VOCs Emitted by Foliage of Grapevine cv. Isabella for Prospecting Innovative Cropping Systems

et al. 2022

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Volatile organic compounds play an important role in communication within plants as well as with other organisms. In this work we identified the volatile organic compounds (VOCs) emitted from the foliage of the grapevine cv. Isabella, a largely known hybrid of Vitis vinifera × Vitis labrusca. Our data show 25 VOCs emitted by cv. Isabella. Different compound classes were found, including alcohols, hydrocarbons, esters, terpenes, ketones, and a green leaf volatile (GLV). The study highlighted differences between volatile profiles for diurnal and nocturnal treatments. The compounds: trans-3-dodecene, 5,5 dibutylnonane, ethyl 2-methyllactate, 2-hexanol, 3-ethyl-2-heptanol, 3-nonanol, and 2-nonanol, have not been previously reported for Vitis vinifera foliage. Notably, eight compounds emitted by cv. Isabella, 1-heptanol, 1-octanol, 2-hexanol, 2-nonanone, β-pinene, camphene, cis-hexenyl acetate, and phenethyl alcohol, are of relevant interest for their role in plant defense. New knowledge on the emission of these compounds in cv. Isabella can help to understand the mechanisms of pathogen tolerance of this genotype and could be an important step in prospecting innovative cropping systems.

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Magnaporthe oryzae cell wall hydrolysate induces ROS and fungistatic VOCs in rice cell cultures

Cited by 8 publications

References 41 publications

Aroma volatile analyses and 2AP characterization at various developmental stages in Basmati and Non-Basmati scented rice (Oryza sativa L.) cultivars

Aroma volatile analyses and 2AP characterization at various developmental stages in Basmati and Non-Basmati scented rice (Oryza sativa L.) cultivars

Inhibition of Fungal Growth and Induction of a Novel Volatilome in Response to Chromobacterium vaccinii Volatile Organic Compounds

Characterization of VOCs Emitted by Foliage of Grapevine cv. Isabella for Prospecting Innovative Cropping Systems

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