Magnesium: does it reduce ischemia/reperfusion injury in an adnexal torsion rat model?

Abstract

AimThe aim of the present study was to assess the protective effects of magnesium sulfate (MgSO4) on ischemia/reperfusion (I/R) induced ovarian damage in a rat ovarian torsion model.MethodsForty-two female Sprague Dawley rats were included in the study. They were divided into six groups as Group 1, sham; Group 2, bilateral ovarian torsion; Group 3, bilateral ovarian torsion–detorsion; Group 4, MgSO4–sham; Group 5, MgSO4–bilateral ovarian torsion; Group 6, bilateral ovarian torsion–MgSO4–detorsion. Both torsion and detorsion periods lasted 3 hours. In Groups 4, 5 and 6, MgSO4 (600 mg/kg) was administered by intraperitoneal route 30 minutes before sham operation, torsion and detorsion, respectively. At the end of the study period, both ovaries were removed. One of the ovaries was used for histopathological analyses and the other for biochemical analyses.ResultsIn the torsion–detorsion group, all the histopathological scores were higher compared to the sham and torsion only group (p<0.05). Administration of MgSO4 only caused significant decrease in the inflammatory cell scores of the torsion–detorsion group (p<0.05). MgSO4, whether given before torsion or before detorsion, suppressed malondialdehyde levels when compared to the untreated groups (p<0.01 and p<0.001, respectively). Glutathione peroxidase activities were significantly higher in the MgSO4 applied torsion and detorsion groups than Groups 2 and 3 (p<0.05, for both). Administration of MgSO4 also caused an increase in glutathione levels in the torsion and detorsion groups compared to the torsion only and detorsion only groups (p<0.05, for both). Also, total oxidant status levels decreased in the MgSO4 applied torsion and detorsion groups compared to the untreated corresponding ones (p<0.01 and p<0.001, respectively). MgSO4 significantly decreased the Oxidative Stress Index levels in the torsion–detorsion group compared to Group 2 (p<0.001).ConclusionHistopathological and biochemical analysis revealed that prophylactic treatment with MgSO4 reduces the changes observed in I/R injury in a rat model.

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“…Lipid peroxidation causes loss of cell membrane integrity, increased permeability to ions, and cell damage. Therefore, inhibiting or reducing the lipid peroxidation process may help prevent tissue damage (26,27). Our study found that MDA levels increased in torsion and torsion-detorsion groups compared to the sham group.…”

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“…[ 6 ] For this reason, performing detorsion procedures is critical to maintain the viability of ischemic ovarian tissue. [ 7 ] However, these procedures can also induce adverse changes in the tissue. Reoxygenation of hypoxic tissue leads to the formation of excessive reactive oxygen species (ROS).…”