Magnesium ions regulate mesenchymal stem cells population and osteogenic differentiation: A fuzzy agent-based modeling approach

Nourisa, Jalil; Zeller‐Plumhoff, Berit; Helmholz, Heike; Luthringer-Feyerabend, Bérengère; Ivannikov, Vladimir; Willumeit‐Römer, Regine

doi:10.1016/j.csbj.2021.07.005

Cited by 16 publications

(22 citation statements)

References 78 publications

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“…Thus, a stronger effect of magnesium pyrophosphate on cellular activity could be observed after seeding of cells on cement surfaces, but on the other hand, this effect needs to be thoroughly analyzed as the accuracy of the results can be affected by incorrect surface structure. Numerical simulation to predict cellular processes verified experimental data on the stimulatory effect of Mg at 3–6 mM concentration on proliferation and ALP activity as well as OC inhibition as a late differentiation marker of MSCs [ 61 ], but this level was not reached in C1MP or C2MP cement extracts; however, the local ion concentration may be higher in close contact between cements and cells or tissues. Magnesium (3 mM) was found to be able to effectively reduce β-glycerol phosphate-induced calcification in rat vascular smooth muscle cells by regulating ALP and OP gene expression [ 62 ].…”

Section: Discussionmentioning

confidence: 79%

Characterization of Tetracalcium Phosphate/Monetite Biocement Modified by Magnesium Pyrophosphate

et al. 2022

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Magnesium pyrophosphate modified tetracalcium phosphate/monetite cement mixtures (MgTTCPM) were prepared by simple mechanical homogenization of compounds in a ball mill. The MgP2O7 was chosen due to the suitable setting properties of the final cements, in contrast to cements with the addition of amorphous (Ca, Mg) CO3 or newberite, which significantly extended the setting time even in small amounts (corresponding ~to 1 wt% of Mg in final cements). The results showed the gradual dissolution of the same amount of Mg2P2O7 phase, regardless of its content in the cement mixtures, and the refinement of formed HAP nanoparticles, which were joined into weakly and mutually bound spherical agglomerates. The compressive strength of composite cements was reduced to 14 MPa and the setting time was 5–10 min depending on the composition. Cytotoxicity of cements or their extracts was not detected and increased proliferative activity of mesenchymal stem cells with upregulation of osteopontin and osteonectin genes was verified in cells cultured for 7 and 15 days in cement extracts. The above facts, including insignificant changes in the pH of simulated body fluid solution and mechanical strength close to cancellous bone, indicate that MgTTCPM cement mixtures could be suitable biomaterials for use in the treatment of bone defects.

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Section: Discussionmentioning

confidence: 79%

Characterization of Tetracalcium Phosphate/Monetite Biocement Modified by Magnesium Pyrophosphate

et al. 2022

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“…By contrast, higher Mg concentrations appeared cytotoxic [ 51 , 52 ]. Of note, a recent reported computer model, calibrated using experimental data, showed that Mg ions within the range of 3–6 mM increased proliferation and early differentiation, whereas high levels caused deleterious effects [ 53 ].…”

Section: Resultsmentioning

confidence: 99%

Simulating In Vitro the Bone Healing Potential of a Degradable and Tailored Multifunctional Mg-Based Alloy Platform

et al. 2022

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This work intended to elucidate, in an in vitro approach, the cellular and molecular mechanisms occurring during the bone healing process, upon implantation of a tailored degradable multifunctional Mg-based alloy. This was prepared by a conjoining anodization of the bare alloy (AZ31) followed by the deposition of a polymeric coating functionalized with hydroxyapatite. Human endothelial cells and osteoblastic and osteoclastic differentiating cells were exposed to the extracts from the multifunctional platform (having a low degradation rate), as well as the underlying anodized and original AZ31 alloy (with higher degradation rates). Extracts from the multifunctional coated alloy did not affect cellular behavior, although a small inductive effect was observed in the proliferation and gene expression of endothelial and osteoblastic cells. Extracts from the higher degradable anodized and original alloys induced the expression of some endothelial genes and, also, ALP and TRAP activities, further increasing the expression of some early differentiation osteoblastic and osteoclastic genes. The integration of these results in a translational approach suggests that, following the implantation of a tailored degradable Mg-based material, the absence of initial deleterious effects would favor the early stages of bone repair and, subsequently, the on-going degradation of the coating and the subjacent alloy would increase bone metabolism dynamics favoring a faster bone formation and remodeling process and enhancing bone healing.

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“…In recent years, an increasing number of studies have been using ABM to simulate cell and molecule interactions for the investigation of the patterns of various tissue growth. [41][42][43] We use an idealized model of tissue regeneration following damage to demonstrate the application of CppyABM (see Figure 4). In this model, a square simulation domain consisting of a 1000 × 1000 grid is created, where each patch has a length of 15 μm which corresponds to the average cell size and only accommodates one agent at a time.…”

Section: Agent-based Simulation Of Cellular Interactionsmentioning

confidence: 99%

“…In recent years, an increasing number of studies have been using ABM to simulate cell and molecule interactions for the investigation of the patterns of various tissue growth 41–43 . We use an idealized model of tissue regeneration following damage to demonstrate the application of CppyABM (see Figure 4).…”

Section: Showcases Of Cppyabmmentioning

confidence: 99%

CppyABM: An open‐source agent‐based modeling library to integrate C++ and Python

2022

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Agent-based modeling (ABM) has been extensively used to study the collective behavior of systems emerging from the interaction of numerous independent individuals called agents. Python and C++ are commonly used for ABM thanks to their unique features; the latter offers superior performance while the former provides ease-of-use and rich libraries in data science, visualization, and machine learning. We present the framework CppyABM that unifies these features by providing identical ABM semantic and development styles in both C++ and Python as well as the essential binding tools to expose a certain functionality from C++ to Python. The binding feature allows users to tailor and further extend a type or function within Python while it is originally defined in C++. Using CppyABM, users can choose either C++ or Python depending on their expertise and the specialty of the model or combine them to benefit from the advantages of both languages simultaneously. We provide showcases of CppyABM capabilities using several examples in computational biology, ecology, and virology. These examples are implemented in different formats using either C++ or Python or a combination of both to provide a comparison between the performance of implementation scenarios. The results of the example show a clear performance advantage of the models entirely or partly implemented in C++ compared to purely Python-based implementations.

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Magnesium ions regulate mesenchymal stem cells population and osteogenic differentiation: A fuzzy agent-based modeling approach

Abstract: Graphical abstract

Cited by 16 publications

References 78 publications

Characterization of Tetracalcium Phosphate/Monetite Biocement Modified by Magnesium Pyrophosphate

Characterization of Tetracalcium Phosphate/Monetite Biocement Modified by Magnesium Pyrophosphate

Simulating In Vitro the Bone Healing Potential of a Degradable and Tailored Multifunctional Mg-Based Alloy Platform

CppyABM: An open‐source agent‐based modeling library to integrate C++ and Python

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