Magnesium lithospermate B improves myocardial function and prevents simulated ischemia/reperfusion injury-induced H9c2 cardiomyocytes apoptosis through Akt-dependent pathway

Quan, Wei; Wu, Bin; Bai, Yan; Zhang, Xiaohong; Yin, Jiaqi; Xi, Miaomiao; Guan, Yue; Shao, Qing; Chen, Yi‐Chen; Wu, Q.; Wen, Aidong

doi:10.1016/j.jep.2013.11.036

Cited by 26 publications

(17 citation statements)

References 29 publications

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“…It has been reported that the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway is an amenable pharmacological target for CVD protection, and it achieves its effect mainly through regulating caspase activation, Bcl-2 family activity, and so on (Wang J. et al, 2015). In the past few years, many traditional Chinese medicines have been claimed to be useful for controlling CVD, particularly for the injury induced by ischemia (Quan et al, 2014;Zheng et al, 2014). Therefore, searching for anti-necrosis and anti-apoptotic compounds with minimal side effects from traditional Chinese medicines probably is an ideal way for CVD patients, and to help create a much safer and more effective treatment.…”

Section: Introductionmentioning

confidence: 99%

Protective mechanisms of hypaconitine and glycyrrhetinic acid compatibility in oxygen and glucose deprivation injury

Wang

Wan

Zhou

et al. 2017

J. Zhejiang Univ. Sci. B

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This study investigated the protective effect of the compatibility of hypaconitine (HA) and glycyrrhetinic acid (GA) on H9c2 cells under oxygen and glucose deprivation (OGD)-induced injury, and the possible mechanisms. We found that HA+GA significantly improved pathology and morphology of the nucleus and ultrastructure of H9c2 cells under OGD as determined by Hoechst 33342 staining and transmission electron microscopy (TEM) tests. It also reduced the releases of lactate dehydrogenase (LDH), creatine kinase-myocardial band isoenzyme (CK-MB), and aspartate transaminase (AST) from the cultured supernatant of H9c2 cells, which were tested by enzyme-linked immune sorbent assay (ELISA) kits. In addition, it lessened the apoptotic rate as determined by a fluorescein isothiocyanate-annexin V/propidium iodide (FITC-AV/PI) double staining assay. It was also found that HA+GA might regulate the protein expression associated with the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway. Overall, the study demonstrated that HA+GA protected H9c2 cells against OGD-induced injury, and the signaling mechanism might be related to the PI3K/Akt signaling pathway.

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Section: Introductionmentioning

confidence: 99%

Protective mechanisms of hypaconitine and glycyrrhetinic acid compatibility in oxygen and glucose deprivation injury

Wang

Wan

Zhou

et al. 2017

J. Zhejiang Univ. Sci. B

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“…Up to date, more than 50 ingredients have been isolated and identified from the aqueous extracts of Danshen. Among them, MLB has attracted the most attention because it is a major aqueous extract ingredient of Danshen and preserves the most pharmacological activities of Danshen (Chen et al, 2011;Wu and Wang, 2012), such as anti-oxidation (Quan et al, 2013b), anti-inflammation and anti-apoptosis (Quan et al, 2013a(Quan et al, , 2014. Furthermore, recent studies have demonstrated that MLB possesses the property of anti-excitotoxicity (Xiao et al, 2013), but the underlying mechanisms remain poorly understood.…”

Section: Discussionmentioning

confidence: 99%

“…MLB possesses multiple pharmacological activities among which anti-oxidation (Quan et al, 2013b), anti-inflammation (Quan et al, 2013a), and anti-apoptosis (Quan et al, 2014) are believed to be responsible for, at least partially, the neuroprotective effects against ischemic stroke. To date, most of studies focused on the antioxidative activity of MLB .…”

Section: Introductionmentioning

confidence: 99%

Beneficial effect of magnesium lithospermate B on cerebral ischemia–reperfusion injury in rats involves the regulation of miR-107/glutamate transporter 1 pathway

Yang

Luo

Ren

et al. 2015

European Journal of Pharmacology

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“…MLB was reported to protect against the ischemia/ reperfusion-induced injury in the rat brain in vivo and in vitro (Quan et al, 2014;Song et al, 2014). MLB has also been demonstrated to inhibit lipopolysaccharide-induced expression of inflammatory molecules (Zhao et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Antidepressant-like effects of magnesium lithospermate B in a rat model of chronic unpredictable stress

Quan

Liu

Zhang

et al. 2015

Pharmaceutical Biology

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Materials and methods: Sprague-Dawley rats were subjected to the chronic unpredictable stress (CUS) protocol. Rats in the control group received no CUS during the whole experiment. In the model group, rats were exposed to CUS for 7 weeks. From the beginning of the 5th week, model group rats were randomly grouped and subjected to different treatments. In the experiment, control and model group rats were intraperitoneally (i.p.) injected with saline. MLB was dissolved in saline to give a final concentration, and the rats were injected (i.p.) with 15, 30, or 60 mg/kg MLB once a day for 3 weeks. Results: MLB administration significantly reduced: (1) the immobility time in the forced swimming test (19 s, p50.05); (2) the immobility time in the tail suspension test (76.3 s, p50.05); (3) the corticosterone (CORT) concentrations in the serum (21.7 nmol/L, p40.05); (4) the pro-inflammatory cytokine levels in the serum -TNF-a (92.1 pg/ml, p50.05), IL-1b (86.9 pg/ ml, p50.05), and IL-6 (93.8 pg/ml, p50.05); (5) pro-inflammatory cytokine levels in tissue -TNF-a (3.2 pg/mg protein, p50.05), IL-1b (1.5 pg/mg protein, p40.05), and IL-6 (6.3 pg/mg protein, p50.05); and (6) phospho-NF-kB (1.6, p50.05) and phospho-IkB-a (0.4, p50.05) expression in tissue. Discussion and conclusion: The results suggested that MLB might exert therapeutic actions on depression-like behavior and the HPA axis hyperactivity in CUS rats, and the mechanisms underlying the antidepressant-like effects of MLB might be mediated by regulation of the expression of NF-kB and IkB-a in rats.

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Magnesium lithospermate B improves myocardial function and prevents simulated ischemia/reperfusion injury-induced H9c2 cardiomyocytes apoptosis through Akt-dependent pathway

Cited by 26 publications

References 29 publications

Protective mechanisms of hypaconitine and glycyrrhetinic acid compatibility in oxygen and glucose deprivation injury

Protective mechanisms of hypaconitine and glycyrrhetinic acid compatibility in oxygen and glucose deprivation injury

Beneficial effect of magnesium lithospermate B on cerebral ischemia–reperfusion injury in rats involves the regulation of miR-107/glutamate transporter 1 pathway

Antidepressant-like effects of magnesium lithospermate B in a rat model of chronic unpredictable stress

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