Magnesium sulfate protects testis against unilateral varicocele in Rat

Abstract

The aim of this study was to investigate the effect of magnesium sulfate (MgSO 4 ) on experimental unilateral varicocele-induced in rats. Sixty male Wistar rats were randomly divided into six experimental groups (n = 10). The Control group had no received any medications and surgery. The Sham group had no received any medication, abdominal cavity was opened without varicocele-induced. Varicocele group: abdominal cavity was opened, varicocele done without any medication treatment. In group 4 abdominal cavity was opened, varicocele-induced then animals orally treated with MgSO 4 (25 mg/kg) for 42 days. The groups 5 and 6 were similar to group 4, except animals received 50 and 100 mg/kg of MgSO 4 , respectively. At the end of days 21 and 42, the abdomen was opened, the left testis extracted for histopathological studies. Also, from the cauda epididymis semen samples were collected to determine malondialdehyde, superoxide dismutase and glutathione peroxidase values. According to the results, there was a significant difference in testis damage grade in Varicocele group compared to the Control group (P < 0.05). The MgSO 4 significantly improved testis damage grade on day 42 (P < 0.05). The MgSO 4 treatment, dose dependently improved seminiferous tubules with many spermatocytes in the seminiferous tubules in experimental unilateral varicocele established rats after 21 and 42 days (P < 0.05). Also, administration of the MgSO 4 via a dose dependent manner significantly normalized malondialdehyde, superoxide dismutase and glutathione peroxidase values to the physiologic level in varicocele-induced rats after 21 and 42 days (P < 0.05). These results suggest administration of the MgSO 4 protects testis against unilateral varicocele in rat.

Effects of egg yolk and soybean lecithin on sperm quality determined by computer‐assisted sperm analysis and confocal laser scanning microscope in chilled canine sperm

“…; Asghari et al . ). Furthermore, Ca 2+ ions also have an important role in intramitochondrial metabolism and energy production in cells (McCormack & Denton ).…”

“…These factors may help to explain that T-M-EY extenders contain several mineral cations, such as Na + , K + , Mg 2+ , Ca 2+ , which are the main ions of seminal plasma, whose important functions are to maintain osmotic balance, form parts of principal enzymes relating to sperm metabolism and sperm function (C ß evik et al 2007;Juyena & Stelletta 2012;Smith et al 2018). In particular, Mg 2+ has a vital function in modulating the regulation of K + (Na-K pump) and Ca 2+ (Owczarzy et al 2008;Smith et al 2018) as well as playing an essential role in enzymatic reactions involving anaerobic glycolysis and energy release from ATP for sperm activities (Wong et al 2001;Asghari et al 2016). Furthermore, Ca 2+ ions also have an important role in intramitochondrial metabolism and energy production in cells (McCormack & Denton 1989).…”

Positive Effects of the Nutraceutical Association of Lycopene and Selenium in Experimental Varicocele

“…Among these substances, vitamins (C, D, E) [30][31][32][33], coenzyme Q10 [34], L-carnitine [35], zinc [36], magnesium [37], silymarin [38], berberine [39], thymoquinone [40], and resveratrol [41] were evaluated, showing a positive role for their antioxidant or antiapoptotic characteristics.…”

Dietary supplements in the management of varicocele‐induced infertility: A review of potential mechanisms

“…In an animal study, the dietary Mg 2 significantly increased the levels of androgenic enzymes in testis of adult rats and levels of testosterone in serum, and subsequently, the progressive development of genital system was reported (Chandra et al, 2013). In an animal study, the oral administration of MgSO 4 (25, 50, and 100 mg/kg for 42 days) significantly improved the testis damage and increased the number of spermatocytes in the seminiferous tubules by normalising the MDA, SOD and GPx values in VCL-induced rats (Asghari et al, 2018). In a randomised, prospective, controlled study, the efficacy of an antioxidant diet (containing 0.01 mg/kg Mg 2+ ) on the quality of sperm parameters and the rate of pregnancies was assessed in the men with persistent oligospermia (sperm concentration of 5-20 million/ml) six months after treatment with surgical technique of retrograde embolism.…”