Magnet-assisted electrochemical immunosensor based on surface-clean Pd-Au nanosheets for sensitive detection of SARS-CoV-2 spike protein

Electrochemical immunosensors using electrodeposited gold nanostructures for detecting the S proteins from SARS-CoV and SARS-CoV-2

“…The electrodes were then rinsed using MES buffer (100 mmol L −1 , pH 6.0) and dried using N 2 . Twenty microliters of 1 µg mL −1 anti-protein S antibodies (PBS, 0.1 mol L −1 , pH 7.0) was added to the electrode for 1 h. The antibody's concentration applied is within the concentration range reported in the literature for the manufacture of immunosensors used for SARS-CoV-2 detection, which range from 1 ng L −1 to 100 µg L −1 [31,32,[35][36][37][38][39][40]. Electrodes were washed using PBS (0.1 mol L −1 , pH 7.0) and dried with an N 2 gas stream.…”

A comparison of conventional and advanced electroanalytical methods to detect SARS-CoV-2 virus: A concise review

“…8 C ( Torres et al, 2021 ). Similarly, in the literature few other electrochemical based sensors based on spike protein detection are reported ( Ali et al, 2021 ; Mahari et al, 2020 ; Mojsoska et al, 2021 ; Nascimento et al, 2022 ; Abrego-Martinez et al, 2022 ; Zhao et al, 2022 ; Jiang et al, 2021 ; Aydın et al, 2021 ).…”

SARS-CoV-2 electrochemical immunosensor based on the spike-ACE2 complex

“…Although other biosensors with slightly lower LODs have been reported in the literature [ 58 , 59 ], our immunosensor has good enough analytical properties for practical applications being advantageous in terms of easiness of sample processing without the need to modify electrodes and use robust and specialized equipment. Remarkable, MBs preconcentrates the spike protein at the surface of the SPAuEs, simplifies washing steps and reduces assays time.…”