Magnetic beads–assisted fluorescence aptasensing approach based on dual DNA tweezers for detection of ochratoxin A and fumonisin B1 in wine and corn

Qu, Chao; Zhao, Luyang; He, Xing; Yu, Songcheng; Wei, Min

doi:10.1007/s00216-021-03635-7

Cited by 11 publications

(5 citation statements)

References 37 publications

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“…Chen et al created a colloidal gold-based lateral flow immunoassay using modified gold nanobeads to detect FB1 [28]. Furthermore, Qu et al created a magnetic beads-assisted fluorescence aptasensing method to detect FB1 [29]. Table 1 shows comparison data between current work and previously reported results for FB1 detection.…”

Section: Introductionmentioning

confidence: 85%

Graphene oxide-Mn₃O₄ nanocomposites for advanced electrochemical biosensor for fumonisin B1 detection

et al. 2023

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Occurrence of mycotoxins in food samples threat to its safety issue due to the presence of high toxicity and carcinogenic behavior, thus requiring highly sensitive and selective detection. Herein, the trimanganese tetraoxide (Mn3O4) nanoparticles in combination with graphene oxide (GO) nanocomposite were used to enhance the electrochemical performance for fabrication of electrochemical biosensor for fumonisin B1 (FB1) detection. The various characterization tools were used to validate the fabrication of GOMn3O4 nanocomposites. To fabricate the electrochemical biosensor on an indium tin oxide (ITO) coated glass substrate, a thin film of GOMn3O4 nanocomposite was prepared using electrophoretic deposition (EPD) technique, and antibodies (ab-FB1) were immobilized onto the electrode for selective FB1 detection. The differential pulse voltammetry (DPV) technique was used to observe the sensing performance. The non-binding sites of the ab-FB1 on the immunoelectrode were blocked with bovine serum albumin (BSA). The biosensor electrode was fabricated as BSA/ab-FB1/GOMn3O4/ITO for the detection of FB1. The sensitivity of the biosensor was obtained as 10.08 µA mL ng-1 cm-2 in the detection range of 1 pg mL-1 to 800 ng mL-1 with a limit of detection (LOD) of 0.195 pg mL-1. In addition, the recovery of BSA/ab-FB1/GOMn3O4/ITO immunoelectrodes was also performed on sweet corn samples and is calculated to be 98.91%.

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Section: Introductionmentioning

confidence: 85%

Graphene oxide-Mn₃O₄ nanocomposites for advanced electrochemical biosensor for fumonisin B1 detection

et al. 2023

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“…Some metal ions are also present in food and severely threaten human health because of illegal addition or environmental pollution during food production. In research on DNA‐fueled molecular machines, metal ions usually serve as initiators for DNA deoxyribozymes (Gül et al., 2019; Qu et al., 2021; Xiong et al., 2020) (DNA zymes), endowing a special DNA sequence with the cleavage properties of enzymes. For instance, Yang and Wu et al.…”

Section: Application Of Dna Tweezers In Food Testingmentioning

confidence: 99%

“…I G U R E 3 Schematic diagram of detection principle based on three-dimensional DNA tweezers: (a) schematic diagram of detection principle of ochratoxin A (OTA) and zearalenone (ZEN) in food with three-dimensional DNA tweezers; (B) schematic diagram of detection principle of OTA and aflatoxin B1 (FB1) in food with three-dimensional DNA tweezers; and (c) dual DNA tweezer detection is a schematic of Cu 2+ and Mg 2+ principles in food.environmental pollution during food production. In research on DNA-fueled molecular machines, metal ions usually serve as initiators for DNA deoxyribozymes(Gül et al, 2019;Qu et al, 2021;Xiong et al, 2020) (DNA zymes), endowing a special DNA sequence with the cleavage properties of enzymes. For instance, Yang and Wu et al (Silverman, 2016) developed an ultrasensitive programcontrolled dual DNA tweezer for the simultaneous ultrasensitive detection of Cu 2+ and Mg 2+ , where the used DNA enzymes showed relatively high ion specificity, with a reliable detection model and less background interference.…”

mentioning

confidence: 99%

Application of DNA‐fueled molecular machines in food safety testing

Ma,

Chen,

Zhang

et al. 2024

Comp Rev Food Sci Food Safe

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Food is consumed by humans, which is indispensable to human life. Therefore, considerable attention of the whole society has been paid to food safety. Over the last few years, dramatic social development has brought new challenges to food safety, making developing new and quick methods for on‐site food safety testing an important necessity. As a result, DNA‐fueled molecular machines, characterized by high efficiency, accuracy, and sensitivity in testing, have come into the spotlight, based on which sensors can be constructed to detect toxic and harmful substances in food products. This study reviewed recent research on several DNA‐fueled molecular machines, including DNA tweezers, DNA walkers, and DNA origami, for rapidly detecting toxic and harmful substances. Based on the above studies, the sensitivity and timeliness of several DNA molecular machines were summarized and compared, and the development prospect of DNA fuel molecular machines in the field of food safety detection was prospected.

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“…The cDNA sequences that were disassociated by competitive binding were used to trigger the dual‐DNA tweezer system. This resulted system led to the fluorescence quench of two separate fluorophores, yielding LODs of 0.029 and 0.061 ng/mL for OTA and FB 1 , respectively (Qu et al., 2021). By utilizing quenching groups instead of quenching nanomaterials, this established fluorescence approach notably decreased in interfering factors and showed good specificity during the analysis of mycotoxins in red wine and corn samples.…”

Section: Mnp‐assisted Simultaneous Detection Of Multiple Mycotoxinsmentioning

confidence: 99%

Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

Gao,

Zhou,

Zhang

et al. 2023

Comp Rev Food Sci Food Safe

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Mycotoxin contamination of food crops is a global challenge due to their unpredictable occurrence and severe adverse health effects on humans. Therefore, it is of great importance to develop effective tools to prevent the accumulation of mycotoxins through the food chain. The use of magnetic nanoparticle (MNP)‐assisted biosensors for detecting mycotoxin in complex foodstuffs has garnered great interest due to the significantly enhanced sensitivity and accuracy. Within such a context, this review includes the fundamentals and recent advances (2020–2023) in the area of mycotoxin monitoring in food matrices using MNP‐based aptasensors and immunosensors. In this review, we start by providing a comprehensive introduction to the design of immunosensors (natural antibody or nanobody, random or site‐oriented immobilization) and aptasensors (techniques for aptamer selection, characterization, and truncation). Meanwhile, special attention is paid to the multifunctionalities of MNPs (recoverable adsorbent, versatile carrier, and signal indicator) in preparing mycotoxin‐specific biosensors. Further, the contribution of MNPs to the multiplexing determination of various mycotoxins is summarized. Finally, challenges and future perspectives for the practical applications of MNP‐assisted biosensors are also discussed. The progress and updates of MNP‐based biosensors shown in this review are expected to offer readers valuable insights about the design of MNP‐based tools for the effective detection of mycotoxins in practical applications.

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Magnetic beads–assisted fluorescence aptasensing approach based on dual DNA tweezers for detection of ochratoxin A and fumonisin B1 in wine and corn

Cited by 11 publications

References 37 publications

Graphene oxide-Mn₃O₄ nanocomposites for advanced electrochemical biosensor for fumonisin B1 detection

Graphene oxide-Mn₃O₄ nanocomposites for advanced electrochemical biosensor for fumonisin B1 detection

Application of DNA‐fueled molecular machines in food safety testing

Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

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Magnetic beads–assisted fluorescence aptasensing approach based on dual DNA tweezers for detection of ochratoxin A and fumonisin B1 in wine and corn

Cited by 11 publications

References 37 publications

Graphene oxide-Mn3O4 nanocomposites for advanced electrochemical biosensor for fumonisin B1 detection

Graphene oxide-Mn3O4 nanocomposites for advanced electrochemical biosensor for fumonisin B1 detection

Application of DNA‐fueled molecular machines in food safety testing

Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

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Graphene oxide-Mn₃O₄ nanocomposites for advanced electrochemical biosensor for fumonisin B1 detection

Graphene oxide-Mn₃O₄ nanocomposites for advanced electrochemical biosensor for fumonisin B1 detection