Magnetic beads-based DNAzyme recognition and AuNPs-based enzymatic catalysis amplification for visual detection of trace uranyl ion in aqueous environment

Zhang, Hongyan; Lin, Ling; Zeng, Xiaoxue; Ruan, YaJuan; Wu, Yongning; Lin, Minggui; He, Ying; Fu, FengFu

doi:10.1016/j.bios.2015.11.024

Cited by 59 publications

(24 citation statements)

References 34 publications

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“…The DNAzyme is found to exhibit high selectivity to specific metal ion against the co-existing interfering metal ions 29,30 . Attributing to the highly specific binding ability to specific metal ion, the DNAzyme has been used in the detection of metal ions [31][32][33] . However, currently, there is no DNA-based adsorbent for the recovery of soluble minerals from seawater, and the recognition mechanism of DNAzyme to specific metal ions, including uranyl, is still unclear.…”

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confidence: 99%

DNA nano-pocket for ultra-selective uranyl extraction from seawater

et al. 2020

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Extraction of uranium from seawater is critical for the sustainable development of nuclear energy. However, the currently available uranium adsorbents are hampered by co-existing metal ion interference. DNAzymes exhibit high selectivity to specific metal ions, yet there is no DNA-based adsorbent for extraction of soluble minerals from seawater. Herein, the uranyl-binding DNA strand from the DNAzyme is polymerized into DNA-based uranium extraction hydrogel (DNA-UEH) that exhibits a high uranium adsorption capacity of 6.06 mg g−1 with 18.95 times high selectivity for uranium against vanadium in natural seawater. The uranium is found to be bound by oxygen atoms from the phosphate groups and the carbonyl groups, which formed the specific nano-pocket that empowers DNA-UEH with high selectivity and high binding affinity. This study both provides an adsorbent for uranium extraction from seawater and broadens the application of DNA for being used in recovery of high-value soluble minerals from seawater.

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confidence: 99%

DNA nano-pocket for ultra-selective uranyl extraction from seawater

et al. 2020

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“…Also, the colorimetric sensors using gold nanoparticles were introduced [ 21 ]. However, there have been few studies on visual detection techniques and most of the conventional colorimetric detection methods need the immobilization of DNAzyme on gold nanoparticles [ 21 – 23 ].…”

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confidence: 99%

Giant Catalytic DNA Particles for Simple and Intuitive Detection of Pb2+

Kim

Lee

2016

Nanoscale Res Lett

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DNAzymes have been extensively studied as biosensors because of their unique functionality of cleaving substrate in the presence of metal ion cofactors. However, there are only a few reports on visual detection using gold nanoparticles. Here, we synthesized the DNAzyme microparticle (DzMP) (~1 μm) via rolling circle amplification for detection of Pb2+ without the help of other materials. Then, the substrate strands were labeled with two different fluorophores (6-carboxyfluorescein and Cy5) to visualize the DzMPs and to monitor the separation of substrate strands. Because of their large size, the decline in the number of fluorescent particles in the presence of Pb2+ could be successfully demonstrated by a fluorescence microscopy, presenting a new platform for heavy metal detection.Electronic supplementary materialThe online version of this article (doi:10.1186/s11671-016-1462-1) contains supplementary material, which is available to authorized users.

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“…Zhang and coworkers recently reported a bioassay that used two structures: DNAzyme-functionalized magnetic beads (MB) for target recognition and AuNPs for catalytic signal amplification [84]. As shown in Figure 7, the first structure involves a biotin-modified substrate strand that is immobilized on streptavidin-coated magnetic beads and forms a duplex with a UO 2 2+ -specific DNAzyme.…”

Section: Peroxidases As Colorimetric Signal Transducermentioning

confidence: 99%

Integrating Deoxyribozymes into Colorimetric Sensing Platforms

Chang

Zakaria

Deng

et al. 2016

Sensors

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Biosensors are analytical devices that have found a variety of applications in medical diagnostics, food quality control, environmental monitoring and biodefense. In recent years, functional nucleic acids, such as aptamers and nucleic acid enzymes, have shown great potential in biosensor development due to their excellent ability in target recognition and catalysis. Deoxyribozymes (or DNAzymes) are single-stranded DNA molecules with catalytic activity and can be isolated to recognize a wide range of analytes through the process of in vitro selection. By using various signal transduction mechanisms, DNAzymes can be engineered into fluorescent, colorimetric, electrochemical and chemiluminescent biosensors. Among them, colorimetric sensors represent an attractive option as the signal can be easily detected by the naked eye. This reduces reliance on complex and expensive equipment. In this review, we will discuss the recent progress in the development of colorimetric biosensors that make use of DNAzymes and the prospect of employing these sensors in a range of chemical and biological applications.

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Magnetic beads-based DNAzyme recognition and AuNPs-based enzymatic catalysis amplification for visual detection of trace uranyl ion in aqueous environment

Cited by 59 publications

References 34 publications

DNA nano-pocket for ultra-selective uranyl extraction from seawater

DNA nano-pocket for ultra-selective uranyl extraction from seawater

Giant Catalytic DNA Particles for Simple and Intuitive Detection of Pb2+

Integrating Deoxyribozymes into Colorimetric Sensing Platforms

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