Magnetic field responsive drug release from magnetoliposomes in biological fluids

Abstract

The final fate of nano-scaled drug delivery systems into the body is highly affected by their interaction with proteins in biological fluids (serum, plasma, etc.). Nanocarriers dispersed in biological fluids bear a protein ''corona'' that covers their surface. Thus, it is extremely important to evaluate the drug release efficiency also in the biological environment where protein-nanocarrier complexes are formed. The purpose of this work is to determine how drug release from lipid vesicle carriers is influenced by the interaction with serum proteins, highlighting the importance to test the effectiveness of such systems in the biological milieu. In particular, this paper describes the magnetically triggered release behaviour of magnetoliposomes (MLs) dispersed both in aqueous physiological buffer and in bovine serum at two different concentrations (10% and 55% v/v) upon exposure to a low-frequency alternating magnetic field (LF-AMF). We studied the release from MLs loaded with two types of magnetic nanoparticles (MNPs): citrate coated Fe 3 O 4 and oleic acid coated g-Fe 2 O 3 . The permeability in the above-mentioned fluids was evaluated in terms of the fluorescence self-quenching of carboxyfluorescein (CF) entrapped inside the liposome aqueous pool. The results showed a strong reduction of the release in biological fluids, in particular at high serum concentration. We related this decrease to the formation of protein-liposome complexes that, under LF-AMF exposure, are subjected to destabilization and tend to form aggregates.Our results clearly highlight the importance of testing the release efficiency of self-assembled drug delivery systems in biological fluids, in order to understand their behaviour in the presence of proteins and biomolecules.

Evidence of Protein Adsorption in Pegylated Liposomes: Influence of Liposomal Decoration

“…In this aspect, Lundqvist et al [ 31 ] have reported that even for a specific material type, the size of the particle, and its surface modification can completely alter the protein corona and probably their biological impact. Corona proteins can physically mask the nanoparticle surface, potentially affecting the therapeutic effect of the molecules bound to the nanoparticle surface [ 32 ]. On another hand, it has also been described that cellular uptake is strongly related to the nature of the proteins that constitute protein corona [ 33 ].…”

Pseudo-zwitterionic microvesicles for sustained urea release

“…Such anomalous behaviour has been attributed to vesicle destabilization and subsequent healing of the system. 26 In another experiment. the system was sonicated for different periods of times at 293.15 K and percent urea release obtained as a function of time is presented in Fig.…”

Spatially Specific Liposomal Cancer Therapy Triggered by Clinical External Sources of Energy

“…A study demonstrated selective hypothermia in vivo and in vitro using magnetoliposomes under a low-frequency AMF to promote lipid membrane permeability from local heating. However, the relatively high heating of the composite particles led to concerns of overheating normal body tissues [142]. In order to overcome injury to healthy tissues caused by the overheating of the particles, other groups embedded the MNPs within the drug carrier to minimize heating of the tissue directly for their drug release studies.…”