Magnetic Helical Microswimmers Functionalized with Lipoplexes for Targeted Gene Delivery

Qiu, Fuming; Fujita, Satoshi; Mhanna, Rami; Zhang, Zifeng; Simona, Benjamin R.; Nelson, Bradley J.

doi:10.1002/adfm.201403891

Cited by 318 publications

(235 citation statements)

References 40 publications

Supporting

Mentioning

234

Contrasting

Order By: Relevance

“…The functionalities of shape-programmable materials are especially appealing for miniature devices whose overall dimensions are smaller than 1 cm as these materials could significantly augment their locomotion and manipulation capabilities. The development of highly functional miniature devices is enticing because, despite having only simple rigid-body motions (34)(35)(36) and gripping capabilities (37), existing miniature devices have already been used across a wide range of applications pertaining to microfluidics (38,39), microfactories (40,41), bioengineering (42,43), and health care (35,44).…”

mentioning

confidence: 99%

Shape-programmable magnetic soft matter

Lum

Zhou

Dong

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

518

475

View full text Add to dashboard Cite

Shape-programmable matter is a class of active materials whose geometry can be controlled to potentially achieve mechanical functionalities beyond those of traditional machines. Among these materials, magnetically actuated matter is particularly promising for achieving complex time-varying shapes at small scale (overall dimensions smaller than 1 cm). However, previous work can only program these materials for limited applications, as they rely solely on human intuition to approximate the required magnetization profile and actuating magnetic fields for their materials. Here, we propose a universal programming methodology that can automatically generate the required magnetization profile and actuating fields for soft matter to achieve new time-varying shapes. The universality of the proposed method can therefore inspire a vast number of miniature soft devices that are critical in robotics, smart engineering surfaces and materials, and biomedical devices. Our proposed method includes theoretical formulations, computational strategies, and fabrication procedures for programming magnetic soft matter. The presented theory and computational method are universal for programming 2D or 3D time-varying shapes, whereas the fabrication technique is generic only for creating planar beams. Based on the proposed programming method, we created a jellyfish-like robot, a spermatozoid-like undulating swimmer, and an artificial cilium that could mimic the complex beating patterns of its biological counterpart.programmable matter | multifunctional materials | soft robots | magnetic actuation | miniature devices S hape-programmable matter refers to active materials that can be controlled by heat (1-5), light (6, 7), chemicals (8-13), pressure (14, 15), electric fields (16, 17), or magnetic fields (18-33) to generate desired folding or bending. As these materials can reshape their geometries to achieve desired time-varying shapes, they have the potential to create mechanical functionalities beyond those of traditional machines (1, 15). The functionalities of shape-programmable materials are especially appealing for miniature devices whose overall dimensions are smaller than 1 cm as these materials could significantly augment their locomotion and manipulation capabilities. The development of highly functional miniature devices is enticing because, despite having only simple rigid-body motions (34-36) and gripping capabilities (37), existing miniature devices have already been used across a wide range of applications pertaining to microfluidics (38, 39), microfactories (40, 41), bioengineering (42, 43), and health care (35, 44).Among shape-programmable matter, the magnetically actuated materials are particularly promising for creating complex timevarying shapes at small scales because their control inputs, in the form of magnetic fields, can be specified not only in magnitude but also in their direction and spatial gradients. Furthermore, as they can be fabricated with a continuum magnetization profile, m, along their bodies, these magne...

show abstract

mentioning

confidence: 99%

Shape-programmable magnetic soft matter

Lum

Zhou

Dong

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

518

475

View full text Add to dashboard Cite

show abstract

“…Drug release was achieved by the photothermal effect, induced by applying an external NIR light stimulus that interacted with the Au segment (Table 1) [53]. Bioinspired magnetically propelled helical microswimmers were functionalized with calcein-loaded liposomes [68] and with lipoplexes-containing pDNA [54] for in vitro targeted drug delivery and gene transfection to single cells ( Figure 2C & Table 1), respectively. Selective delivery of siRNA was also demonstrated by using an acoustically propelled nanowire motor ( Figure 2D & Table 1) [55].…”

Section: Physical Actuationmentioning

confidence: 99%

Micro- and nano-motors: the New Generation of Drug Carriers

2018

View full text Add to dashboard Cite

Micro-and nano-motors are emerging as novel drug delivery platforms, offering advantages such as rapid drug transport, high tissue penetration and motion controllability. They can be propelled and/or guided by endogenous (i.e., chemotaxis) or exogenous stimuli (e.g., ultrasound, magnetic fields, light) toward the area of interest. Moreover, such stimuli can be used to trigger the release of a therapeutic payload when the motor reaches certain location in order to improve the drug targeting. In this review article, we highlight medically oriented micro-/nano-motors, in particular the ones created for targeted drug delivery, and discuss their current limitations and possibilities toward in vivo applications. Drug-delivery systems have greatly evolved since 1952, when the first sustained release systems were introduced (see the graphical abstract) [1]. In the 1980s, self-regulated drug release carriers as well as the first drug delivery nanostructures were developed. From then on, research in this field has been focused on improving the targeting and systemic circulation of those carriers as well as to study different nanoparticle-based drug formulations, topics which have been widely reviewed in the literature [2][3][4][5][6][7]. Several nanocarriers (between 5 and 200 nm diameter) have been explored for therapy (e.g. inorganic, polymeric, liposomes, nanocrystals, nanotubes and dendrimers) [8], many of them already approved by the Food and Drug Administration (FDA) to treat, for example, neurovascular diseases, neurological cancers and neurodegenerative maladies [9]. The study of their pharmacokinetics has led to the creation of new carriers with reduced drug dose and protection from efflux or degradation [10]. Most of these nanocarriers include sophisticated surface biofunctionalization and coatings like polyethylene glycol (PEG) [11] or biomimetic modifications, [12] to increase their specificity and circulation time [13]. However, challenges still remain for those carriers such as the improvement of their targeting (currently only about 0.7% -median -of the administrated nanoparticle dose is found to be delivered to a solid tumor [14]), their penetration ability, drug loading capacity and delivery on the subcellular level. Other type of drug carriers are the cellular ones, which have many advantages such Graphical abstract:

show abstract

“…In either case, the load can be released after an external trigger or through degradation over time. The example shows a Ni‐coated helix rotating in a magnetic field, which is able to deliver genes in lipoplexes …”

Section: Bioinspired Synthetic Microswimmersmentioning

confidence: 99%

Biohybrid and Bioinspired Magnetic Microswimmers

Bente

Codutti

Bachmann

et al. 2018

Small

120

View full text Add to dashboard Cite

Many motile microorganisms swim and navigate in chemically and mechanically complex environments. These organisms can be functionalized and directly used for applications (biohybrid approach), but also inspire designs for fully synthetic microbots. The most promising designs of biohybrids and bioinspired microswimmers include one or several magnetic components, which lead to sustainable propulsion mechanisms and external controllability. This Review addresses such magnetic microswimmers, which are often studied in view of certain applications, mostly in the biomedical area, but also in the environmental field. First, propulsion systems at the microscale are reviewed and the magnetism of microswimmers is introduced. The review of the magnetic biohybrids and bioinspired microswimmers is structured gradually from mostly biological systems toward purely synthetic approaches. Finally, currently less explored parts of this field ranging from in situ imaging to swarm control are discussed.

show abstract

Magnetic Helical Microswimmers Functionalized with Lipoplexes for Targeted Gene Delivery

Cited by 318 publications

References 40 publications

Shape-programmable magnetic soft matter

Shape-programmable magnetic soft matter

Micro- and nano-motors: the New Generation of Drug Carriers

Biohybrid and Bioinspired Magnetic Microswimmers

Contact Info

Product

Resources

About