Magnetic Lateral Flow Immunoassays

Moyano, Amanda; Serrano-Pertierra, Esther; Salvador, María Lázaro; Martínez-García, J.C.; Rivas, M.; Blanco‐López, M. Carmen

doi:10.3390/diagnostics10050288

Cited by 78 publications

(45 citation statements)

References 96 publications

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“…This is possible due to the physical and chemical characteristics of magnetic nanoparticles, which can be detected by means of magnetometric sensors. Magnetic LFIAs have been reported to detect different important analytes in numerous fields [ 35 ]. Moreover, magnetic labels not only allow quantification, but they can also be employed for immunomagnetic separation enabling isolation and enrichment of analyte [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker Detection

Moyano

Serrano-Pertierra

Duque

et al. 2021

Sensors

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Colorectal cancer (CRC) is the third leading cause of cancer death and the fourth most common cancer in the world. Colonoscopy is the most sensitive test used for detection of CRC; however, their procedure is invasive and expensive for population mass screening. Currently, the fecal occult blood test has been widely used as a screening tool for CRC but displays low specificity. The lack of rapid and simple methods for mass screening makes the early diagnosis and therapy monitoring difficult. Extracellular vesicles (EVs) have emerged as a novel source of biomarkers due to their contents in proteins and miRNAs. Their detection would not require invasive techniques and could be considered as a liquid biopsy. Specifically, it has been demonstrated that the amount of CD147 expressed in circulating EVs is significant higher for CRC cell lines than for normal colon fibroblast cell lines. Moreover, CD147-containing EVs have been used as a biomarker to monitor response to therapy in patients with CRC. Therefore, this antigen could be used as a non-invasive biomarker for the detection and monitoring of CRC in combination with a Point-of-Care platform as, for example, Lateral Flow Immunoassays (LFIAs). Here, we propose the development of a quantitative lateral flow immunoassay test based on the use of magnetic nanoparticles as labels coupled to inductive sensor for the non-invasive detection of CRC by CD147-positive EVs. The results obtained for quantification of CD147 antigen embedded in EVs isolated from plasma sample have demonstrated that this device could be used as a Point-of-Care tool for CRC screening or therapy monitoring thanks to its rapid response and easy operation.

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Section: Introductionmentioning

confidence: 99%

Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker Detection

Moyano

Serrano-Pertierra

Duque

et al. 2021

Sensors

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“…The conventional immunochromatographic tests are the basis of the actual serological and antigen tests used to monitor and diagnosis of COVID-19. In recent years, research in this field is giving rise to a new generation of LFIAs relying on MNPs, and it could be considered that magnetic LFIA are emerging as powerful tool for diagnostics [ 19 ]. In this direction, previous studies from this research group showed the successful development of these kind of tests for prostatic cancer antigen, exosomes and histamine [ 20 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Microemulsion Synthesis of Superparamagnetic Nanoparticles for Bioapplications

Salvador

Gutiérrez

Noriega

et al. 2021

IJMS

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Superparamagnetic nanoparticles have seen increased potential in medical and environmental applications. Their preparation is traditionally made by the coprecipitation method, with limited control over the particle size distribution. Microemulsion methods could be advantageous due to the efficient control of the size, shape, and composition of the nanoparticles obtained. Water-in-oil (W/O) microemulsions consist of aqueous microdomains dispersed in a continuous oil phase, stabilized by surfactant molecules. These work as nanoreactors where the synthesis of the desired nanoparticles takes place through a co-precipitation chemical reaction. In this work, superparamagnetic magnetite nanoparticles with average diameters between 5.4 and 7.2 nm and large monodispersity have been synthesized through precipitation in a W/O microemulsion, with Cetyl Trimethyl Ammonium Bromide (CTAB) as a main surfactant, 1-butanol as a cosurfactant, and with 1-hexanol as the continuous oily phase. The optimization of the corresponding washing protocol has also been established since a strict control is required when using these materials for bioapplications. Their applicability in those has been proved by their encapsulation in liposomes, being tested as signal enhancers for lateral flow immunoassays by using the affinity neutravidin-biotin model system. Due to their magnetic behaviour, they were also tested for magnetic separation. These novel materials have been found to be useful for analytical applications requiring high sensitivity and the removal of interferences.

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“…Lateral flow immunoassays (LFIA), also called immunochromatographic tests, have been established as POC tests in recent years due to the rapid, low-cost, simple detection [5]. They are the basis for the well-known pregnancy test, but they can be designed for different applications in biomedicine, toxicology, food, agriculture, and environmental fields [6,7]. They consist of a membrane of nitrocellulose, where bioreceptors are immobilized.…”

Section: Introductionmentioning

confidence: 99%

Lipid–Polymer Hybrids Encapsulating Iron-Oxide Nanoparticles as a Label for Lateral Flow Immunoassays

et al. 2021

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The feasibility of using Superparamagnetic Iron Oxide Nanoparticles (SPIONs) encapsulated by lipid–polymer nanoparticles as labels in lateral flow immunoassays (LFIA) was studied. First, nanoparticles were synthesized with average diameters between 4 and 7 (nm) through precipitation in W/O microemulsion and further encapsulated using lipid–polymer nanoparticles. Systems formulated were characterized in terms of size and shape by DLS (Nanozetasizer from Malvern) and TEM. After encapsulation, the average size was around (≈20 and 50 nm). These controlled size agglomerates were tested as labels with a model system based on the biotin–neutravidin interaction. For this purpose, the encapsulated nanoparticles were conjugated to neutravidin using the carbodiimide chemistry, and the LFIA was carried out with a biotin test line. The encapsulated SPIONs showed that they could be promising candidates as labels in LFIA test. They would be useful for immunomagnetic separations, that could improve the limits of detection by means of preconcentration.

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Magnetic Lateral Flow Immunoassays

Cited by 78 publications

References 96 publications

Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker Detection

Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker Detection

Microemulsion Synthesis of Superparamagnetic Nanoparticles for Bioapplications

Lipid–Polymer Hybrids Encapsulating Iron-Oxide Nanoparticles as a Label for Lateral Flow Immunoassays

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