Magnetic microbead-based enzyme immunoassay for ovalbumin using hydrodynamic voltammetry and fluorometric detection

Kuramitz, Hideki; Halsall, H. Brian; Heineman, William R.

doi:10.1039/c2ay05738g

Cited by 17 publications

(11 citation statements)

References 28 publications

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“…Various methods of complex liquid and particle treatment such as AC and DC electroosmosis, 1, 2 electrophoresis, 3 dielectrophoresis, 4 digital addressing, 5 electrocoalescence 6 or electrochemical sensing 7 are driven by external electric fields imposed on specific parts of the microfluidic systems. For example, charged water droplets dispersed in an oil phase can be addressed by means of DC electric field into specific parts of a microfluidic structure.…”

Section: Introductionmentioning

confidence: 99%

Oscillatory motion of water droplets in kerosene above co-planar electrodes in microfluidic chips

et al. 2014

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We experimentally observed oscillatory motion of water droplets in microfluidic systems with coplanar microelectrodes under imposed DC electric fields. Two-electrode arrangement with no bipolar electrode and eight-electrode arrangement with six bipolar microelectrodes were investigated. Kerosene was used as the continuous phase. We studied the dependences of the oscillation frequency on the electric field intensity and ionic strength of the water phase. We found that the electric field dependence is strongly nonlinear and discussed possible reasons of this phenomenon, e.g., the droplet deformation at electrode edges that affects the charge transfer between the electrode and droplet or the interplay between the Coulomb force on free charge and the dielectrophoretic force. Our experiments further revealed that the oscillation frequency decreases with growing salt concentration in the two-electrode arrangement, but increases in the eight-electrode arrangement, which was attributed to surface tension related processes and electrochemical processes on the bipolar electrodes. Finally, we analyzed the effects of the electric field on the oscillatory motion by means of a simplified mathematical model. It was shown that the electric force imposed on the droplet charge is the key factor to induce the oscillations and the dielectrophoretic force significantly contributes to the momentum transfer at the electrode edges. For the same electric field strength, the model is able to predict the same oscillation frequency as that observed in the experiments.

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Section: Introductionmentioning

confidence: 99%

Oscillatory motion of water droplets in kerosene above co-planar electrodes in microfluidic chips

et al. 2014

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“…Another notable benefit associated with the use of this system is its rapid response due to obtain fast equilibrium. Various reports exist on the use of this detection technique, for example in the magnetic microbead‐based assay for ovalbumin , in the soil enzyme assay , in the gene toxicity assay , and in microalgal bioassay .…”

Section: Resultsmentioning

confidence: 99%

Adsorptive Voltammetry for the Determination of Ochratoxin A Using Enrichment Effect by Cationic Surfactants

et al. 2018

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A rapid and simple electrochemical method was utilized for the determination of the presence of classic fungal metabolite ochratoxin A (OTA) based on differential pulse adsorptive voltammetry, using the carbon nanotube paste (CNTP) electrode. OTA showed a clear oxidation peak, and the CNTP electrode exhibited a more sensitive response to OTA oxidation than the glassy carbon, the plastic formed carbon, and the carbon paste electrodes. The addition of long‐chain cationic surfactants, such as benzethonium chloride, cetyltrimethylammonium bromide, and benzyldimethyltetradecylammonium chloride (zephiramine), enhanced the electrochemical response of OTA. Under optimized experimental conditions, the calibration plots are linear in the 5×10−6−5×10−5 M OTA concentration range, and the limit of detection is 1.1×10−6 M. In order to improve the selectivity for OTA determination, we attempted to combine this adsorptive stripping voltammetric technique with “sequestration electrochemistry,” based on the specific interaction occurring between bovine serum albumin (BSA) and OTA. The peak current of OTA oxidation decreased substantially as a result of OTA‐BSA binding. Furthermore, it was demonstrated that the application of this proposed method of hydrodynamic adsorptive voltammetry, using a micro‐droplet and a rotating disk electrode, improves the approach's sensitivity and reduces the time and sample volume required for the analysis.

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“…One of these methods involved the fabrication of a water-dispersible molecular imprinted conductive polyaniline particle that was used to perform the electrochemical detection of OVA [40]. The calibration curve of OVA concentration was linear and ranged from 2.2 × 10 À 14 to 1.0 × 10 À 7 M with a lower detection limit of 2.0 × 10 À 14 M. In another method, the electrode response of paminophenol produced from p-aminophenyl galactopyranoside via the enzymatic reaction of β-galactosidase was recorded using voltammetry for an OVA detection limit of 2.1 × 10 À 9 M [41]. Eissa et al developed a graphenebased label-free voltammetric immunosensor for the detection of OVA.…”

Section: Electrochemical Sensing Of Ova Using Peptide-1-immobilized Bmentioning

confidence: 99%

Electrochemical Sensing of Ovalbumin Based on the Interaction between Lysozyme Origin/Tyrosine‐rich Peptides Modified on Magnetic Beads and Oligothreonine/Ovalbumin‐origin Peptide

et al. 2019

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We developed a highly sensitive electrochemical system for the sensing of ovalbumin (OVA). Lysozyme origin/tyrosine‐rich peptides (RNRCKGTDVQAWY4C) were immobilized on magnetic beads, and the competitive reaction between OVA and oligothreonine/OVA origin peptide probe (T8VLLPDEVSG) could then be measured. In a previous study, the detection of OVA at the 10−13 M level was achieved using RNRCKGTDVQAWY4C‐modified beads via a cross‐linker. To improve the sensitivity to OVA, this system uses T8VLLPDEVSG peptide probe to measure the interaction to RNRCKGTDVQAWY4C immobilized on magnetic beads. The peak of Y4C actually was an electron‐transfer peptide, which represented the oxidation of a phenolic hydroxyl group. First, we confirmed that the oxidation response of Y4C was increased based on an improvement in the electron transfer accessibility by oligothreonine. Next, T8VLLPDEVSG peptide probe was used for the electrochemical sensing of OVA in solutions that contained consistent amounts of RNRCKGTDVQAWY4C on magnetic beads. As a result, the peak current decreased as the concentration of OVA increased. The sensitivity to OVA was improved compared with the use of only RNRCKGTDVQAWY4C on magnetic beads. The OVA detection level was 10−14 M, which approximates the results from antibody‐antigen reactions. Consequently, the proposed system is a powerful new concept in protein sensing.

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Magnetic microbead-based enzyme immunoassay for ovalbumin using hydrodynamic voltammetry and fluorometric detection

Cited by 17 publications

References 28 publications

Oscillatory motion of water droplets in kerosene above co-planar electrodes in microfluidic chips

Oscillatory motion of water droplets in kerosene above co-planar electrodes in microfluidic chips

Adsorptive Voltammetry for the Determination of Ochratoxin A Using Enrichment Effect by Cationic Surfactants

Electrochemical Sensing of Ovalbumin Based on the Interaction between Lysozyme Origin/Tyrosine‐rich Peptides Modified on Magnetic Beads and Oligothreonine/Ovalbumin‐origin Peptide

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