Magnetic molecular imprinted polymers as a tool for isolation and purification of biological samples

Kwaśniewska, Katarzyna; Gadzała‐Kopciuch, Renata; Buszewski, Bogusław

doi:10.1515/chem-2015-0137

Cited by 17 publications

(8 citation statements)

References 49 publications

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“…Magnetic nanoparticles (MNP) are becoming more popular in extracting different analytes from various samples [ 26 , 27 , 28 , 29 , 30 , 31 , 32 ]. The surfaces of MNPs are usually modified and functionalized with different molecules.…”

Section: Types Of Supports Used In Ogns Purification and Separationmentioning

confidence: 99%

Oligonucleotides Isolation and Separation—A Review on Adsorbent Selection

Studzińska

Nuckowski

Buszewski

2022

IJMS

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Oligonucleotides have many important applications, including as primers in polymerase chain reactions and probes for DNA sequencing. They are proposed as a diagnostic and prognostic tool for various diseases and therapeutics in antisense therapy. Accordingly, it is necessary to develop liquid chromatography and solid phase extraction methods to separate oligonucleotides and isolate them from biological samples. Many reviews have been written about the determination of these compounds using the separation technique or sample preparation for their isolation. However, presumably, there are no articles that critically review the adsorbents used in liquid chromatography or solid phase extraction. The present publication reviews the literature from the last twenty years related to supports (silica, polymers, magnetic nanoparticles) and their modifications. The discussed issues concern reversed phase (alkyl, aromatic, cholesterol, mixed ligands), ion-exchange (strong and weak ones), polar (silica, polyhydroxy, amide, zwitterionic), and oligonucleotide-based adsorbents.

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Section: Types Of Supports Used In Ogns Purification and Separationmentioning

confidence: 99%

Oligonucleotides Isolation and Separation—A Review on Adsorbent Selection

Studzińska

Nuckowski

Buszewski

2022

IJMS

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“…Magnetic separation technology, in which polymers are prepared using MIP fabrication on the surface of a magnetic substrate, has been widely used in recent years for separation and extraction applications [ 8 , 9 , 10 , 11 ], such as in the field of drug analysis in biological fluids [ 12 , 13 , 14 , 15 , 16 , 17 ], analysis of compounds in the environment [ 9 , 10 , 11 , 18 , 19 , 20 , 21 , 22 ], analysis of compounds in food [ 8 , 23 , 24 , 25 ] and analysis of compounds in plants and other naturally occurring products [ 26 , 27 , 28 , 29 ]. Magnetic molecular imprinted solid phase extraction (MMI-SPE) is a new solid phase extraction (SPE) procedure based on the use of magnetic sorbents [ 8 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…MMIP preparation begins with the preparation of a magnetic core, commonly called magnetite, using a co-precipitation technique between ferric chloride (FeCl 2 ·H 2 O) or ferrous sulphate (FeSO 4 ·7H 2 O) and iron(III) chloride hexahydrate (FeCl 3 ·6H 2 O), which can be achieved under basic conditions of 80–100 °C [ 14 ]. After the magnetite is formed, its surface is modified, either by silanisation or by adding a surfactant such as ethylene glycol or oleic acid, enhancing the amphoteric properties of the magnetite surface and improving its interaction with polar solutions.…”

Section: Introductionmentioning

confidence: 99%

Factors Affecting the Analytical Performance of Magnetic Molecularly Imprinted Polymers

et al. 2022

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During the last few years, separation techniques using molecular imprinting polymers (MIPs) have been developed, making certain improvements using magnetic properties. Compared to MIP, Magnetic molecularly imprinted polymers (MMIPs) have high selectivity in sample pre-treatment and allow for fast and easy isolation of the target analyte. Its magnetic properties and good extraction performance depend on the MMIP synthesis step, which consists of 4 steps, namely magnetite manufacture, magnetic coating using modified components, polymerization and template desorption. This review discusses the factors that will affect the performance of MMIP as a selective sorbent at each stage. MMIP, using Fe3O4 as a magnetite core, showed strong superparamagnetism; it was prepared using the co-precipitation method using FeCl3·6H2O and FeCl2·H2O to obtain high magnetic properties, using NH4OH solution added for higher crystallinity. In magnetite synthesis, the use of a higher temperature and reaction time will result in a larger nanoparticle size and high magnetization saturation, while a higher pH value will result in a smaller particle size. In the modification step, the use of high amounts of oleic acid results in smaller nanoparticles; furthermore, determining the correct molar ratio between FeCl3 and the shielding agent will also result in smaller particles. The next factor is that the proper ratio of functional monomer, cross-linker and solvent will improve printing efficiency. Thus, it will produce MMIP with high selectivity in sample pre-treatment.

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“…In recent years, in the world of nanoparticles, molecularly imprinted polymers (MIP) have been synthesized as new innovative sorbents. The development of sorbents and the imprinted particle allows proposing innovative solutions in separation and purification [ 1 , 2 , 3 , 4 ]. MIPs are characterized as artificial bio-receptors or as antibody–antigen systems due to the mechanism based on the selective binding of specific molecules in a special cavity, which is similar to the lock–key model [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Magnetic Molecularly Imprinted Polymer Sorbents for Isolation of Parabens from Breast Milk

et al. 2020

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Magnetic molecularly imprinted polymers (MMIPs) are an invaluable asset in the development of many methods in analytical chemistry, particularly sample preparation. Novel adsorbents based on MMIPs are characterized by high selectivity towards a specific analyte due to the presence of a specific cavity on their polymer surface, enabling the lock–key model interactions to occur. In addition, the magnetic core provides superparamagnetic properties that allow rapid separation of the sorbent from the sample solution. Such a combination of imprinted polymers with a magnetic core has an innovative influence on the development of separation techniques. Hence, the present study describes the synthesis of MMIPs with 17β-estradiol used as a template molecule in the production of imprinted polymers. The as-prepared sorbent was used for a sorption/desorption study of five parabens from breast milk samples. The obtained results were characterized by sorption efficiency exceeding 92%, which shows the high affinity of the analytes to the functional groups on the sorbent. The final determination of the selected analytes was done with high-performance liquid chromatography using a fluorometric detector. The determined linearity ranges for selected parabens were characterized by high determination coefficients (r2 from 0.9992 to 0.9999), and the calculated limit of detection (LOD) and limit of quantification (LOQ) for the identified compounds were low (LOD from 1.1–2.7 ng mL−1; LOQ from 3.6–8.1 ng mL−1), which makes their quantitative analysis in real samples feasible.

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Magnetic molecular imprinted polymers as a tool for isolation and purification of biological samples

Cited by 17 publications

References 49 publications

Oligonucleotides Isolation and Separation—A Review on Adsorbent Selection

Oligonucleotides Isolation and Separation—A Review on Adsorbent Selection

Factors Affecting the Analytical Performance of Magnetic Molecularly Imprinted Polymers

Synthesis of Magnetic Molecularly Imprinted Polymer Sorbents for Isolation of Parabens from Breast Milk

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