Magnetic molecularly imprinted polymer nanoparticles coupled with high performance liquid chromatography for solid‐phase extraction of carvedilol in serum samples

Abstract

Herein, we report a magnetic molecularly imprinted polymers (m-MIPs) using Fe 3 O 4 as a magnetic component, carvedilol as a template molecule for the solid-phase extraction (MISPE) as the sample clean-up technique combined with high-performance liquid chromatography (HPLC) and for the controlled release of carvedilol at different pH values of 1.0 (simulated gastric fluid), 6.8 (simulated intestinal fluid), and 7.4 (simulated biological fluid). The adsorption kinetics was modeled with the pseudo-first-order and pseudosecond-order kinetics, and the adsorption isotherms were fitted with Langmuir and Freundlich models. The performance of the m-MIPs for the controlled release of carvedilol was assessed and results indicated that the magnetic MIPs also have potential applications in controlled drug release. Furthermore, the m-MIPs were applied to the extraction of carvedilol from human blood plasma samples. Carvedilol can be quantified by this method in the 2-350 lg L 21 concentration range. The limit of detection and limit of quantification in plasma samples are 0.13 and 0.45 lg L 21. The results from HPLC showed good precision (3.5% for 50.0 lg L 21 ) and recoveries (between 85 and 93) using m-MIP from human plasma samples.

Computer-aided design of magnetic molecularly imprinted polymer nanoparticles for solid-phase extraction and determination of levetiracetam in human plasma

“…Complex matrices as biological uids constituting blood, plasma or urine contain a number of endogenous components, which if not removed may interfere and adversely affect the separation and identication of analyte(s) of interest. 1,2 Such challenges made sample preparation one of the most important and crucial steps in the analytical process. 3 However, one drawback of sample preparation steps is that they consume around two-thirds of the total analysis time and are considered the major source of error in the overall analytical process.…”

Preparation and Performance of an Estradiol Templated Magnetic Sphere of Molecularly Imprinted Cryogel

“…[5][6][7] The layer can be further modified with hydrophilic and/or biocompatible functional groups, including C=C, -NH-, -Br and sulfonyl, then dipole interaction of core is shielded, and agglomeration of particles is prevented. 3,[8][9][10] Core-shell MMIP can provide specific recognition because of the mesoporous and microporous molecular scale cavities, and can shorten the processing time of sample due to the excellent settling performance and ease to be separated from the matrix through an external magnetic field.…”

“…[5][6][7] The layer can be further modified with hydrophilic and/or biocompatible functional groups, including C=C, -NH-, -Br and sulfonyl, then dipole interaction of core is shielded, and agglomeration of particles is prevented. 3,[8][9][10] Core-shell MMIP can provide specific recognition because of the mesoporous and microporous molecular scale cavities, and can shorten the processing time of sample due to the excellent settling performance and ease to be separated from the matrix through an external magnetic field.11 Core-shell MMIP can increase the contact area of adsorbent and matrix, and thus provide a larger diffusion and mass transfer rate due to its good dispersion.12 Fe 3 O 4 cored core-shell MMIP has been widely applied in solid phase extraction, drug delivery, enzyme immobilization, cell imaging, proteomics, DNA sequencing, bio magnetic separation, immunomagnetic separation, etc.13,14 Wang et al 3 Vol. 29, No.…”

Preparation of magnetic glycoprotein‐imprinted nanoparticles with dendritic polyethyleneimine as a monomer for the specific recognition of ovalbumin from egg white

“…To overcome these troubles, surface imprinting technique with the aid of nano‐solid matrix has been adopted . Particularly, magnetic nanoparticles (MNPs) won the researchers’ eye by reason of simple synthesis, tunable size, plenty of functional groups, stability, nontoxicity, biocompatibility, and paramagnetic.…”