Magnetic nanoparticles coated with carboxymethylated polysaccharide shells—Interaction with human cells

Wotschadlo, Jana; Liebert, Tim; Heinze, Thomas; Wagner, Kerstin; Schnabelrauch, Matthias; Dutz, Silvio; Müller, Robert N.; Steiniger, Frank; Schwalbe, Manuela; Kroll, Torsten; Höffken, K.; Buske, N.; Clement, Joachim H.

doi:10.1016/j.jmmm.2009.02.069

Cited by 33 publications

(36 citation statements)

References 19 publications

(25 reference statements)

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“…Several natural polymers have been successfully used in the formation of stable magnetic particles (An et al, 2003, Gupta and Gupta, 2005, Kim et al, 2003, Ma et al, 2007, Villanueva et al, 2009, Williams et al, 2006, Wotschadlo et al, 2009). Different combinations of styrene-sulfonic acid, vinyl-sulfonic acid, and acrylic acid have also been used to coated magnetic particles to donate a variety of electrostatic and hydrophobic surface properties (Ditsch et al, 2005).…”

Section: Magnetophoretic Cell Separation: Theory and Phenomenamentioning

confidence: 99%

Fundamentals and application of magnetic particles in cell isolation and enrichment: a review

2014

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Magnetic sorting using magnetic beads has become a routine methodology for the separation of key cell populations from biological suspensions. Due to the inherent ability of magnets to provide forces at a distance, magnetic cell manipulation is now a standardized process step in numerous processes in tissue engineering, medicine, and in fundamental biological research. Herein we review the current status of magnetic particles to enable isolation and separation of cells, with a strong focus on the fundamental governing physical phenomena, properties and syntheses of magnetic particles and on current applications of magnet-based cell separation in laboratory and clinical settings. We highlight the contribution of cell separation to biomedical research and medicine and detail modern cell separation methods (both magnetic and non-magnetic). In addition to a review of the current state-of-the-art in magnet-based cell sorting, we discuss current challenges and available opportunities for further research, development and commercialization of magnetic particle-based cell separation systems.

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Section: Magnetophoretic Cell Separation: Theory and Phenomenamentioning

confidence: 99%

Fundamentals and application of magnetic particles in cell isolation and enrichment: a review

2014

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“…For the second synthetic strategy, carboxylic moieties, which are able to react with the primary amino groups of the lPEIs, were introduced to the dextran backbone by carboxymethylation using monochloroacetic acid (MCA) under basic conditions (Sun et al, 2008b;Wotschadlo et al, 2009). The ratios of the reagents (dextran-AGU:MCA:NaOH = 2.2:1:1 or 1:5:10) as well as the reaction times (90 or 300 min) were altered in order to obtain different CM-dex precursors with varying DS.…”

Section: Edc Couplingmentioning

confidence: 99%

Dextran-graft-linear poly(ethylene imine)s for gene delivery: Importance of the linking strategy

Ochrimenko

Vollrath

Tauhardt

et al. 2014

Carbohydrate Polymers

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“…16 Dextran and CMD being highly hydrophilic, flexible, and easy to functionalize, these polymers have also been employed as lowfouling versatile coatings that limit nonspecific adsorption at the surface of surface plasmon resonance (SPR)-based biosensor chips 14 and multiple-well plates for enzyme-linked immunosorbent assays (ELISA). 17 Taken together, those properties make the CMD polymer family an interesting candidate for nanoparticle coating in the context of nucleic acid delivery for gene therapy.…”

Section: ■ Introductionmentioning

confidence: 99%

Tailoring the Surface of a Gene Delivery Vector with Carboxymethylated Dextran: A Systematic Analysis

et al. 2015

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Vous avez des questions? Nous pouvons vous aider. Pour communiquer directement avec un auteur, consultez la première page de la revue dans laquelle son article a été publié afin de trouver ses coordonnées. Si vous n'arrivez pas à les repérer, communiquez avec nous à PublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. Questions? Contact the NRC Publications Archive team atPublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. If you wish to email the authors directly, please see the first page of the publication for their contact information. NRC Publications Archive Archives des publications du CNRCThis publication could be one of several versions: author's original, accepted manuscript or the publisher's version. / La version de cette publication peut être l'une des suivantes : la version prépublication de l'auteur, la version acceptée du manuscrit ou la version de l'éditeur. NRC Publications Record / Notice d'Archives des publications de CNRC:http://nparc.cisti-icist.nrc-cnrc.gc.ca/eng/view/object/?id=ab1c9b68-9dc8-474c-a7ca-0ed13201ac54 http://nparc.cisti-icist.nrc-cnrc.gc.ca/fra/voir/objet/?id=ab1c9b68-9dc8-474c-a7ca-0ed13201ac54 ABSTRACT: Polymeric nanocarriers are attractive nonviral vectors for gene delivery purposes in vivo. For such applications, numerous physiological and subcellular bottlenecks have to be overcome. In that endeavor, each structural feature of nanocarriers can be optimized with respect to its corresponding challenges. Here, we focused on the interface between a model gene delivery nanocarrier and relevant constituents of the physiological environment. We screened a library of carboxymethylated dextrans (CMD) for the electrostatic coating of positively charged nanocarriers. We evaluated the jointed influence of the CMD molecular weight and charge density upon nanocarrier coating with respect to DNase, small ions, plasma proteins, red blood cells, and target cells. A total of 4 out of 26 CMD coated nanocarriers successfully passed every screening assay, but did not yield increased reporter gene expression in target cells compared to uncoated nanocarriers. The fine-tuning of CMD for nanocarrier coating yielded a relevant shortlist of candidates that will be further tested in vivo.

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Magnetic nanoparticles coated with carboxymethylated polysaccharide shells—Interaction with human cells

Cited by 33 publications

References 19 publications

Fundamentals and application of magnetic particles in cell isolation and enrichment: a review

Fundamentals and application of magnetic particles in cell isolation and enrichment: a review

Dextran-graft-linear poly(ethylene imine)s for gene delivery: Importance of the linking strategy

Tailoring the Surface of a Gene Delivery Vector with Carboxymethylated Dextran: A Systematic Analysis

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