Magnetic nanoparticles coated with immobilized alkaline phosphatase for enzymolysis and enzyme inhibition assays

Wang, Siming; Su, Ping; Huang, Jun; Wu, Jingwei; Yang, Yi

doi:10.1039/c3tb00562c

Cited by 55 publications

(35 citation statements)

References 36 publications

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“…Application of metal nanoparticles in this fast-growing field has placed the source to the find the alternative methods to postprandial hyperglycemia. The recent scientific investigation have been reported that metal nanoparticles are being used for enzymolysis and enzyme inhibition assays [11].…”

Section: Introductionmentioning

confidence: 99%

Facile green synthesis of gold nanoparticles from marine algae Gelidiella acerosa and evaluation of its biological Potential

Senthilkumar¹,

Surendran²,

Sudhagar³

et al. 2019

SN Appl. Sci.

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In this present research, the Gold nanoparticles (GA-AuNPs) produced from Gelidiella acerosa, marine algae using green synthesis method was evaluated for its antidiabetic, antibacterial and antioxidant activity. Well-developed GA-AuNPs were effectively characterized using advanced analytical instruments like HRTEM, UV-visible, SEM and XRD. This analysis exposed GA-AuNPs to be present with spherical, hexagonal structure and crystalline nature. The average size of GA-AuNPs particles was estimated to lie between 5.81 nm to 117.59 nm by using Debye-Scherer equation. The enzyme inhibitory property of (IC 50 ) of G. acerosa aqueous extract and synthesized gold nanoparticles from G. acerosa against α amylase and α-glucosidase enzyme was found to be 2.1 ± 0.01, 3.7 ± 0.01 µg/mL and 2.8 ± 0.02, 4.1 ± 0.01, respectively. Further antioxidant assessment of GA-AuNPs were examined using DPPH and FRAP assays, the results exhibited that the strong antioxidant activity were observed in all the concentrations when compared with standard. Finally antibacterial activity was evaluated the GA-AuNPs against gram positive and gram negative bacteria, the strong antibacterial activity was observed in both the bacterial strains when compared with standard antibiotic. The GA-AuNPs from marine algae was exhibited to be strong inhibitory activity against alpha amylase and alpha glucosidase enzyme, antioxidant activity and antibacterial activity, these GA-AuNPs are projected to have potential biological applications.

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Section: Introductionmentioning

confidence: 99%

Facile green synthesis of gold nanoparticles from marine algae Gelidiella acerosa and evaluation of its biological Potential

Senthilkumar¹,

Surendran²,

Sudhagar³

et al. 2019

SN Appl. Sci.

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“…[14][15][16][17][18][19][20][21][22][23][24] For example, Hernandez and coworkers 16 developed an amperometric flow system based on a magnetically immobilized glucose oxidase, for the determination of glucose in blood samples. The amino modified, silica coated, magnetite was used to bind the enzyme with the glutaraldehyde protocol.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Coupling Protocols to Bind Beta-Glucosidase on Magnetic Nanoparticles

Riccò¹,

Doherty²,

Falcaro³

2014

j nanosci nanotechnol

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Beta-Glucosidase has been chosen as a model biomolecule to establish a general protocol for binding enzymes on both ferromagnetic and superparamagnetic nano-particles for sensing applications. Using EDC (1-(3-dimethyl-aminopropyl)-3-ethylcarbodiimide) or SMCC (Succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate) as coupling agents, we compared two different methods for the fabrication of enzyme-decorated magnetic nanoparticles. We identified the best conditions for the preparation of a responsive bioactive magnetic system comparing different covalent bio-grafting protocols. The enzymatic test has been performed using beta-Glucosidase. The systems were characterized using scanning electron microscopy, infrared spectroscopy, and the enzyme loading was measured by a glucose assay in the presence of the enzyme-decorated magnetic particles. Although the faster response of ferromagnetic particles to the magnetic field, the assay results suggested that the superparamagnetic particles are more efficient carriers. In fact, the best enzymatic activity was measured on superparamagnetic systems that have the further advantage of preventing aggregation induced by the residual magnetization. Hence, beta-Glucosidase coated magnetic nanospheres could provide an attractive system suitable for the cleavage and the rapid evaluation of glycoside levels in natural products, measuring the liberated glucose without the need for specialised instrumentation. Moreover, the magnetic particles allow the subsequent collection of enzymes for further analysis, such as its use in portable fast screening kits or devices.

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“…Moreover, they can also be valuable for the fabrication of conductive matrices [47][48][49] or as a platform for enzyme [50,51] or for ligand immobilisation [52]. Large surface areas, chemical inertness and high electrical conductivity are properties that are beneficial for the purposes mentioned above.…”

Section: Nanomaterials and Microbeadsmentioning

confidence: 98%

Utilisation of micro- and nanoscaled materials in microfluidic analytical devices

Monošík

Angnes

2015

Microchemical Journal

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Magnetic nanoparticles coated with immobilized alkaline phosphatase for enzymolysis and enzyme inhibition assays

Cited by 55 publications

References 36 publications

Facile green synthesis of gold nanoparticles from marine algae Gelidiella acerosa and evaluation of its biological Potential

Facile green synthesis of gold nanoparticles from marine algae Gelidiella acerosa and evaluation of its biological Potential

Evaluation of Coupling Protocols to Bind Beta-Glucosidase on Magnetic Nanoparticles

Utilisation of micro- and nanoscaled materials in microfluidic analytical devices

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