Magnetic nanoparticles–serum proteins bioconjugates for binding of irinotecan

Spectrofluorometric and thermal gravimetric study on binding interaction of thiabendazole with hemoglobin on epoxy-functionalized magnetic nanoparticles

“…As shown in Fig. 3A, the globular structure of Hb has been distinguished on the magnetic nanoparticle clutter [13]. After the binding of Tbz to Hb, the morphology has slightly changed (Fig.…”

“…Functionalized SPIONs at the particle concentration range of 2.5-25 mg mL −1 were mixed with 1 mg mL −1 of hemoglobin (Hb) in 2 mL of 20 mM × Tris-HCl (pH 7.4, in 150 mM NaCl) for 2 h at 4°C [13]. Hb immobilized GPTS-SPIONs were separated magnetically from the supernatant.…”

“…Drug solution was added to the appropriate amount of Hb immobilized GPTS-SPIONs in 20 mM × Tris-HCl (pH 7.4). Each mixture was mixed for 30 min at a particle concentration range of 2.5-25 mg mL −1 [13]. The nanoparticles were separated using a 0.5 T magnet.…”

“…Then, the other thermodynamic parameters (ΔS, ΔH and ΔG) can be calculated from Eqs. (11)- (13). Thus, all these parameters are used to compare the stabilities of Tbz-Hb-GPTS- SPIONs and Hb-GPTS-SPIONs (without drug).…”

“…In literature, most studied proteins for drug binding are bovine serum albumin and human serum albumin. However, there are several studies on interactions between hemoglobin and drugs that have been reported by fluorescence and thermogravimetry techniques [12,13]. Identification of interactions between drugs and hemoglobin is very crucial since these interactions may have undesirable effects on their main function.…”