Magnetic Particles Coupled to Disposable Screen Printed Transducers for Electrochemical Biosensing

Yáñez‐Sedeño, Paloma; Campuzano, Susana; Pingarrón, José M.

doi:10.3390/s16101585

Cited by 34 publications

(12 citation statements)

References 96 publications

(108 reference statements)

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“…In addition to the previously mentioned sensor surface modification based on nanomaterials and biomaterials, magnetic beads (MBs) are powerful tools for bioassays because of, for example, the increase of the surface area and the improvement of the sensitivity of the detection method . Their use, combined with modified electrode surfaces improves biomolecule interactions and reduces or/and minimizes the matrix effect of the sample by efficient washing steps .…”

Section: Magnetic Immunoassaysmentioning

confidence: 99%

Electrochemical Biosensing in Cancer Diagnostics and Follow‐up

2018

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In cancer, screening and early detection are critical for the success of the patient's treatment and to increase the survival rate. The development of analytical tools for non‐invasive detection, through the analysis of cancer biomarkers, is imperative for disease diagnosis, treatment and follow‐up. Tumour biomarkers refer to substances or processes that, in clinical settings, are indicative of the presence of cancer in the body. These biomarkers can be detected using biosensors, that, because of their fast, accurate and point of care applicability, are prominent alternatives to the traditional methods. Moreover, the constant innovations in the biosensing field improve the determination of normal and/or elevated levels of tumour biomarkers in patients’ biological fluids (such as serum, plasma, whole blood, urine, etc.). Although several biomarkers (DNA, RNA, proteins, cells) are known, the detection of proteins and circulating tumour cells (CTCs) are the most commonly reported due to their approval as tumour biomarkers by the specialized entities and commonly accepted for diagnosis by medical and clinical teams. Therefore, electrochemical immunosensors and cytosensors are vastly described in this review, because of their fast, simple and accurate detection, the low sample volumes required, and the excellent limits of detection obtained. The biosensing strategies reported for the six most commonly diagnosed cancers (lung, breast, colorectal, prostate, liver and stomach) are summarized and the distinct phases of the sensors’ constructions (surface modification, antibody immobilization, immunochemical interactions, detection approach) and applications are discussed.

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Section: Magnetic Immunoassaysmentioning

confidence: 99%

Electrochemical Biosensing in Cancer Diagnostics and Follow‐up

2018

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“…Besides the electrochemical improvement, nano/micro-sized MBs find plenty of applications due to both their large surface area (useful to load recognition elements) and their magnetic guidance. MBs are easily recovered by means of an external magnetic source, offering great simplifications in both terms of target pre-concentration and interferences reduction [ 36 , 37 ].…”

Section: Nano- and Micro-sized Materials For Improving Detectionmentioning

confidence: 99%

Electrochemical Biosensors for Rapid Detection of Foodborne Salmonella: A Critical Overview

Cinti

Volpe

Piermarini

et al. 2017

Sensors

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Salmonella has represented the most common and primary cause of food poisoning in many countries for at least over 100 years. Its detection is still primarily based on traditional microbiological culture methods which are labor-intensive, extremely time consuming, and not suitable for testing a large number of samples. Accordingly, great efforts to develop rapid, sensitive and specific methods, easy to use, and suitable for multi-sample analysis, have been made and continue. Biosensor-based technology has all the potentialities to meet these requirements. In this paper, we review the features of the electrochemical immunosensors, genosensors, aptasensors and phagosensors developed in the last five years for Salmonella detection, focusing on the critical aspects of their application in food analysis.

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“…In particular, electrochemical magneto-immunosensors have proven to be sensitive, accurate, fast and inexpensive, capable of achieving adequate limits of detection and very suitable as point-of-care tools for decentralized analysis. The use of magnetic beads has been largely exploited in recent years due to their well-known properties such as improved assay kinetics and easy manipulation, while they are perfectly coupled to electrochemical detection [58]. However, magneto-immunosensors [16,17,35,36] in general, and particularly those using electrochemical detection [40], have not been widely explored for PCT determination.…”

Section: Introductionmentioning

confidence: 99%

Magnetic Bead-Based Electrochemical Immunoassays On-Drop and On-Chip for Procalcitonin Determination: Disposable Tools for Clinical Sepsis Diagnosis

et al. 2020

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Procalcitonin (PCT) is a known protein biomarker clinically used for the early stages of sepsis diagnosis and therapy guidance. For its reliable determination, sandwich format magnetic bead-based immunoassays with two different electrochemical detection approaches are described: (i) disposable screen-printed carbon electrodes (SPE-C, on-drop detection); (ii) electro-kinetically driven microfluidic chips with integrated Au electrodes (EMC-Au, on-chip detection). Both approaches exhibited enough sensitivity (limit of detection (LOD) of 0.1 and 0.04 ng mL−1 for SPE-C and EMC-Au, respectively; cutoff 0.5 ng mL−1), an adequate working range for the clinically relevant concentrations (0.5–1000 and 0.1–20 ng mL−1 for SPE-C and EMC-Au, respectively), and good precision (RSD < 9%), using low sample volumes (25 µL) with total assay times less than 20 min. The suitability of both approaches was successfully demonstrated by the analysis of human serum and plasma samples, for which good recoveries were obtained (89–120%). Furthermore, the EMC-Au approach enabled the easy automation of the process, constituting a reliable alternative diagnostic tool for on-site/bed-site clinical analysis.

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Magnetic Particles Coupled to Disposable Screen Printed Transducers for Electrochemical Biosensing

Cited by 34 publications

References 96 publications

Electrochemical Biosensing in Cancer Diagnostics and Follow‐up

Electrochemical Biosensing in Cancer Diagnostics and Follow‐up

Electrochemical Biosensors for Rapid Detection of Foodborne Salmonella: A Critical Overview

Magnetic Bead-Based Electrochemical Immunoassays On-Drop and On-Chip for Procalcitonin Determination: Disposable Tools for Clinical Sepsis Diagnosis

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