Magnetic relaxation switching immunoassay based on “limited-magnitude” particles for sensitive quantification of aflatoxin B1

Lü, Yingying; Chen, Rui; Dong, Yongzhen; Zhao, Weiqi; Ruan, Shilong; Yang, Weihai; Chen, Yiping; Wang, Chengming

doi:10.1016/j.aca.2023.341329

Cited by 10 publications

(4 citation statements)

References 29 publications

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“…Reprinted with permission from Lu et al. (2023). (c) Schematic diagram of Fe 3 O 4 @PDA‐antibody‐mediated microchannel resistance sensor for the detection of deoxynivalenol (DON).…”

Section: Roles and Applications Of Mnps In Mycotoxin Detectionmentioning

confidence: 99%

“…Due to the broad presence of mycotoxins in agricultural products and their processed products, mycotoxin contamination in cereals (maize, oat, rice, millet, wheat, sorghum, buckwheat, barley, etc.) (Jiang et al., 2021; Lu et al., 2023), nuts (peanut, pistachio, cashews, etc.) (Zhu et al., 2020), beverages (milk, beer, coffee, tea, juice, wine, Chinese Baijiu, etc.)…”

Section: Roles and Applications Of Mnps In Mycotoxin Detectionmentioning

confidence: 99%

“…Consequently, an immunocomplex comprising AFB 1 ‐BSA‐PS mm /Ab‐MNP 150 was formed following a competition reaction. A linear negative correlation between T 2 and the amount of MNP 150 was established for calculating AFB 1 in wheat and maize samples (Lu et al., 2023). Additionally, the aggregated MNP nanostructures can also be in a format of Au NPs‐bridged Ag@Au–Fe 3 O 4 (Dong et al., 2021) and PDA‐glued MNPs aggregates (PDA@MNPs) (Zhao, Wang et al., 2021), resulting in a LOD of 3.81 and 0.35 pg/mL for AFB 1 , respectively.…”

Section: Roles and Applications Of Mnps In Mycotoxin Detectionmentioning

confidence: 99%

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Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

Gao,

Zhou,

Zhang

et al. 2023

Comp Rev Food Sci Food Safe

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Mycotoxin contamination of food crops is a global challenge due to their unpredictable occurrence and severe adverse health effects on humans. Therefore, it is of great importance to develop effective tools to prevent the accumulation of mycotoxins through the food chain. The use of magnetic nanoparticle (MNP)‐assisted biosensors for detecting mycotoxin in complex foodstuffs has garnered great interest due to the significantly enhanced sensitivity and accuracy. Within such a context, this review includes the fundamentals and recent advances (2020–2023) in the area of mycotoxin monitoring in food matrices using MNP‐based aptasensors and immunosensors. In this review, we start by providing a comprehensive introduction to the design of immunosensors (natural antibody or nanobody, random or site‐oriented immobilization) and aptasensors (techniques for aptamer selection, characterization, and truncation). Meanwhile, special attention is paid to the multifunctionalities of MNPs (recoverable adsorbent, versatile carrier, and signal indicator) in preparing mycotoxin‐specific biosensors. Further, the contribution of MNPs to the multiplexing determination of various mycotoxins is summarized. Finally, challenges and future perspectives for the practical applications of MNP‐assisted biosensors are also discussed. The progress and updates of MNP‐based biosensors shown in this review are expected to offer readers valuable insights about the design of MNP‐based tools for the effective detection of mycotoxins in practical applications.

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“…Reprinted with permission from Lu et al. (2023). (c) Schematic diagram of Fe 3 O 4 @PDA‐antibody‐mediated microchannel resistance sensor for the detection of deoxynivalenol (DON).…”

Section: Roles and Applications Of Mnps In Mycotoxin Detectionmentioning

confidence: 99%

Section: Roles and Applications Of Mnps In Mycotoxin Detectionmentioning

confidence: 99%

Section: Roles and Applications Of Mnps In Mycotoxin Detectionmentioning

confidence: 99%

See 1 more Smart Citation

Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

Gao,

Zhou,

Zhang

et al. 2023

Comp Rev Food Sci Food Safe

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“…AFB 1 has been deemed a class I carcinogen by the International Agency for Research on Cancer. It is because of its high hazardousness that many countries have set extremely stringent regulations for AFB 1 permitted in food and agricultural products, ranging from 2 to 20 ng/g. As such, a highly efficient detection assay is important to control the harmfulness of AFB 1 .…”

Section: Introductionmentioning

confidence: 99%

Click Reaction-Mediated Fluorescent Immunosensor Based on Cu-MOF Nanoparticles for Ultrasensitive and High-Throughput Detection of Aflatoxin B₁ in Food Samples

Hong,

Zhao,

Pan

et al. 2024

J. Agric. Food Chem.

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Due to the high toxicity of aflatoxin B 1 and its risks to human health, we developed a click reaction-mediated automated fluorescent immunosensor (CAFI) for sensitive detection of aflatoxin B 1 based on the Cu(I)-catalyzed click reaction. With its large specific surface area, a copper-based metal−organic framework (Cu-MOF) was synthesized to adsorb and enrich the copper ion (Cu(II)) and then load the complete antigen (BSA-AFB 1 ). After the immunoreaction, Cu(II) inside the Cu-MOF-Antigen conjugate would be reduced to Cu(I) in the presence of sodium ascorbate, which triggered the click reaction between the fluorescent donor-modified DNA and the receptor-modified complementary DNA to lead to a fluorescence signal readout. The whole reaction steps were finished by the self-developed automated immunoreaction device. This CAFI method showed a limit of detection (LOD) of 0.48 pg/mL as well as a 670-fold enhancement in sensitivity compared to conventional ELISA, revealing its great potential in practical applications and automated detection.

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Mesophilic Argonaute‐Based Single Polystyrene Sphere Aptamer Fluorescence Platform for the Multiplexed and Ultrasensitive Detection of Non‐Nucleic Acid Targets

Lu,

Wen,

Wang

et al. 2023

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The rapid, simultaneous, and accurate identification of multiple non‐nucleic acid targets in clinical or food samples at room temperature is essential for public health. Argonautes (Agos) are guided, programmable, target‐activated, next‐generation nucleic acid endonucleases that could realize one‐pot and multiplexed detection using a single enzyme, which cannot be achieved with CRISPR/Cas. However, currently reported thermophilic Ago‐based multi‐detection sensors are mainly employed in the detection of nucleic acids. Herein, this work proposes a Mesophilic Argonaute Report‐based single millimeter Polystyrene Sphere (MARPS) multiplex detection platform for the simultaneous analysis of non‐nucleic acid targets. The aptamer is utilized as the recognition element, and a single millimeter‐sized polystyrene sphere (PSmm) with a large concentration of guide DNA on the surface served as the microreactor. These are combined with precise Clostridium butyricum Ago (CbAgo) cleavage and exonuclease I (Exo I) signal amplification to achieve the efficient and sensitive recognition of non‐nucleic acid targets, such as mycotoxins (<60 pg mL−1) and pathogenic bacteria (<102 cfu mL−1). The novel MARPS platform is the first to use mesophilic Agos for the multiplex detection of non‐nucleic acid targets, overcoming the limitations of CRISPR/Cas in this regard and representing a major advancement in non‐nucleic acid target detection using a gene‐editing‐based system.

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Magnetic relaxation switching immunoassay based on “limited-magnitude” particles for sensitive quantification of aflatoxin B1

Cited by 10 publications

References 29 publications

Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

Click Reaction-Mediated Fluorescent Immunosensor Based on Cu-MOF Nanoparticles for Ultrasensitive and High-Throughput Detection of Aflatoxin B₁ in Food Samples

Mesophilic Argonaute‐Based Single Polystyrene Sphere Aptamer Fluorescence Platform for the Multiplexed and Ultrasensitive Detection of Non‐Nucleic Acid Targets

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Magnetic relaxation switching immunoassay based on “limited-magnitude” particles for sensitive quantification of aflatoxin B1

Cited by 10 publications

References 29 publications

Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

Magnetic nanoparticle‐based immunosensors and aptasensors for mycotoxin detection in foodstuffs: An update

Click Reaction-Mediated Fluorescent Immunosensor Based on Cu-MOF Nanoparticles for Ultrasensitive and High-Throughput Detection of Aflatoxin B1 in Food Samples

Mesophilic Argonaute‐Based Single Polystyrene Sphere Aptamer Fluorescence Platform for the Multiplexed and Ultrasensitive Detection of Non‐Nucleic Acid Targets

Contact Info

Product

Resources

About

Click Reaction-Mediated Fluorescent Immunosensor Based on Cu-MOF Nanoparticles for Ultrasensitive and High-Throughput Detection of Aflatoxin B₁ in Food Samples