Magnetic resonance and photoacoustic imaging of brain tumor mediated by mesenchymal stem cell labeled with multifunctional nanoparticle introduced via carotid artery injection

Qiao, Yanjiang; Gumin, Joy; MacLellan, Christopher J.; Gao, Feng; Bouchard, Richard R.; Lang, Frederick F.; Stafford, R. Jason; Melancon, Marites P.

doi:10.1088/1361-6528/aaaf16

Cited by 64 publications

(57 citation statements)

References 50 publications

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“…Numerous studies have described flow cytometric evaluation of MSC surface markers in order to assess the differentiation potential of MSCs following nanoparticle internalization. 35,[45][46][47][48][49] In agreement with our findings, their results consistently reported no effects on surface-marker expression or osteogenic and adipogenic differentiation ability. These results showed that nanoparticlelabeling did not alter MSC differentiation or characteristics higher than 50 µg/mL did not enhance cell-uptake efficiency, at 100 µg/mL, the nanoparticles tended to aggregate in the culture medium and adhere to the plate.…”

Section: Characterization Of Mscs Loaded With Iron Oxide Nanoparticlessupporting

confidence: 90%

Iron oxide nanoparticles promote the migration of mesenchymal stem cells to injury sites

Li¹,

Wei²,

Lv³

et al. 2019

IJN

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BackgroundDeveloping new methods to deliver cells to the injured tissue is a critical factor in translating cell therapeutics research into clinical use; therefore, there is a need for improved cell homing capabilities.Materials and methodsIn this study, we demonstrated the effects of labeling rat bone marrow-derived mesenchymal stem cells (MSCs) with fabricated polydopamine (PDA)-capped Fe3O4 (Fe3O4@PDA) superparticles employing preassembled Fe3O4 nanoparticles as the cores.ResultsWe found that the Fe3O4@PDA composite superparticles exhibited no adverse effects on MSC characteristics. Moreover, iron oxide nanoparticles increased the number of MSCs in the S-phase, their proliferation index and migration ability, and their secretion of vascular endothelial growth factor relative to unlabeled MSCs. Interestingly, nanoparticles not only promoted the expression of C-X-C chemokine receptor 4 but also increased the expression of the migration-related proteins c-Met and C-C motif chemokine receptor 1, which has not been reported previously. Furthermore, the MSC-loaded nanoparticles exhibited improved homing and anti-inflammatory abilities in the absence of external magnetic fields in vivo.ConclusionThese results indicated that iron oxide nanoparticles rendered MSCs more favorable for use in injury treatment with no negative effects on MSC properties, suggesting their potential clinical efficacy.

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Section: Characterization Of Mscs Loaded With Iron Oxide Nanoparticlessupporting

confidence: 90%

Iron oxide nanoparticles promote the migration of mesenchymal stem cells to injury sites

Li¹,

Wei²,

Lv³

et al. 2019

IJN

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“…The main and most used are bioluminescent optical imaging (BLI), magnetic resonance imaging (MRI), and nuclear imaging techniques. [50][51][52][53][54][55] For a in-depth review on MSC homing imaging, see Reference 43. In a syngeneic rat model, luciferase charged MSCs, injected in the right common carotid artery, were tracked by in vivo BLI into right frontal lobe, co-localizing with glioma cells. Similar results were observed using a fluorescent vital dye, after injection into the same site.…”

Section: Overview Of Msc Functional Propertiesmentioning

confidence: 99%

“…53 Other technical studies validate the use of gold-coated nanoparticles or ferritin heavy chain expressing MSCs as marker to track by MRI. 50,54 Migration of AT-MSCs to target brain tumor-initiating cells was studied by in vivo BLI analysis using fluorescent magnetic nanoparticles. 52,56 57,58 promoting macrophages transformation from M1 to M2 anti-inflammatory phenotype, 59 or contributing to immune homeostasis.…”

Section: Overview Of Msc Functional Propertiesmentioning

confidence: 99%

Cross talk between mesenchymal and glioblastoma stem cells: Communication beyond controversies

Bajetto

Thellung

Dellacasagrande

et al. 2020

Stem Cells Translational Medicine

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Mesenchymal stem cells (MSCs) can be isolated from bone marrow or other adult tissues (adipose tissue, dental pulp, amniotic fluid, and umbilical cord). In vitro, MSCs grow as adherent cells, display fibroblast-like morphology, and self-renew, undergoing specific mesodermal differentiation. High heterogeneity of MSCs from different origin, and differences in preparation techniques, make difficult to uniform their functional properties for therapeutic purposes. Immunomodulatory, migratory, and differentiation ability, fueled clinical MSC application in regenerative medicine, whereas beneficial effects are currently mainly ascribed to their secretome and extracellular vesicles. MSC translational potential in cancer therapy exploits putative anti-tumor activity and inherent tropism toward tumor sites to deliver cytotoxic drugs. However, controversial results emerged evaluating either the therapeutic potential or homing efficiency of MSCs, as both antitumor and protumor effects were reported. Glioblastoma (GBM) is the most malignant brain tumor and its development and aggressive nature is sustained by cancer stem cells (CSCs) and the identification of effective therapeutic is required. MSC dualistic action, tumor-promoting or tumor-targeting, is dependent on secreted factors and extracellular vesicles driving a complex cross talk between MSCs and GBM CSCs. Tumor-tropic ability of MSCs, besides providing an alternative therapeutic approach, could represent a tool to understand the biology of GBM CSCs and related paracrine mechanisms, underpinning MSC-GBM interactions. In this review, recent findings on the complex nature of MSCs will be highlighted, focusing on their elusive impact on GBM progression and aggressiveness by direct cell-cell interaction and via secretome, also facing the perspectives and challenges in treatment strategies.

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“…Thus far, in oncology, the role of these MRI-guided theranostics is mainly restricted to tracking transplanted cells in animal models ( Table 2). [67][68][69][70][71][72] Intravenous (I.V.) injection is a frequently used medication delivery route and is commonly applied for MSC delivery in both animal models and clinical trials, although extensive MSC recruitment into the tumor site of gliomas upon regional injection into the cerebral hemisphere or intra-arterial injection via the internal carotid artery has been observed in many studies, 71,[73][74][75] the homing ability of systemically injected MSCs toward brain tumors has been less evidenced by cellular imaging, because most MSCs are entrapped in the reticuloendothelial system and pulmonary vasculature after I.V.…”

Section: Mri-tracking Of Msc Tumor-homingmentioning

confidence: 99%

Cellular Magnetic Resonance Imaging with Superparamagnetic Iron Oxide: Methods and Applications in Cancer

Hsu

Sun

Hsieh

2019

SPIN

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Superparamagnetic iron oxide (SPIO) has been used as a contrast agent for magnetic resonance imaging (MRI) since the late 20th century. With the development of SPIO, cellular MRI has been recognized as a suitable and highly sensitive noninvasive modality with great potential to benefit translational research. In addition to its traditional diagnostic property, latest advances have conferred SPIO with multifunctionality. Several SPIO-based theranostic probes with targeting, therapeutic and diagnosis components have been successfully developed. The objective of this brief review is to summarize the characteristics, synthesizing methods, labeling approaches and current applications of SPIO-based cellular MRI in oncology. Herein, we first depict the history, classification and advantages of and the differences between T1- and T2/T2*-based SPIO contrast agents for cancer treatment. Second, we outline current coating materials that render SPIO less toxic and more biocompatible to mammalian cells. Finally, the cell labeling techniques and applications of SPIO-based MRI for tracking mesenchymal stem cell tumor–homing in preclinical models are introduced.

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Magnetic resonance and photoacoustic imaging of brain tumor mediated by mesenchymal stem cell labeled with multifunctional nanoparticle introduced via carotid artery injection

Abstract: Our results demonstrated the feasibility of tracking carotid artery-injected SPIO@Au-labeled MSCs in vivo via MR and PA imaging.

Cited by 64 publications

References 50 publications

Iron oxide nanoparticles promote the migration of mesenchymal stem cells to injury sites

Iron oxide nanoparticles promote the migration of mesenchymal stem cells to injury sites

Cross talk between mesenchymal and glioblastoma stem cells: Communication beyond controversies

Cellular Magnetic Resonance Imaging with Superparamagnetic Iron Oxide: Methods and Applications in Cancer

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