Magnetic resonance imaging in personalized medicine

Wang, Xiaoying; Zhou, Xiaohong Joe

doi:10.1007/s11427-016-0395-3

Cited by 15 publications

(11 citation statements)

References 11 publications

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“…NO, TNF- α , and IL-1 β as important proinflammatory cytokines and inflammatory mediators, were critical in inflammation-associated and immunization-associated response [ 31 ]. Samples with NO, TNF- α , and IL-1 β inhibitory activity have potential to possess immunostimulatory activity.…”

Section: Resultsmentioning

confidence: 99%

Extraction, Characterization, Antioxidant, and Immunostimulatory Activities of Polysaccharides from Hedyotis corymbosa

Lin

Xiong

Zhao

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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In this study, optimization of enzyme-assisted extraction, purification, characterization, and its bioactivities of polysaccharides from Hedyotis corymbosa (HCP) was investigated. It was found that the optimum extraction conditions were 3% of enzyme concentration (X1), 30 of liquid-to-solid ratio (X2), 56°C of extraction temperature (X3), 200W of ultrasonic power (X4), 10 min of extraction time (X5), and 5 of pH value (X6). Under optimum conditions, the experimental yield (4.10 ± 0.16%) was closed to the predicted value (4.02%). The crude HCP was further purified using DEAE-52 and Sephadex G-150 gel column, and a major polysaccharide fraction from HCP, designed as HCP-1a with molecular weight of 33.9 kDa, was obtained. The HCP and HCP-1a were characterized by chemical analysis, FT-IR, and HPLC. For antioxidant activities in vitro, HCP possessed strong hydroxyl radical scavenging, DPPH radical scavenging, and Fe2+ chelating activities. In subsequent immunostimulatory studies, significantly decreased NO, IL-1β, and TNF-α concentrations were observed in both of HCP and HCP-1a treated RAW264.7 cells. Therefore, this study may indicate some insights into the application of polysaccharides from Hedyotis corymbosa as potential natural antioxidants and immunostimulants.

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Section: Resultsmentioning

confidence: 99%

Extraction, Characterization, Antioxidant, and Immunostimulatory Activities of Polysaccharides from Hedyotis corymbosa

Lin

Xiong

Zhao

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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“…Among the latest promising IBD treatments is therapy involving the oral administration of lactic acid bacteria [16, 17]. However, it is still unclear exactly how particular action mechanisms and the therapeutic roles of bacteria are correlated, so the clinical feasibility of this therapy is under debate [18, 19]. We used a mouse model of colitis triggered by DSS to investigate the use of the most popular probiotic, L. plantarum , in IBD treatment.…”

Section: Discussionmentioning

confidence: 99%

The Impact ofLactobacillus plantarumon the Gut Microbiota of Mice with DSS-Induced Colitis

Zhang

Wang

et al. 2019

BioMed Research International

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The pathogenesis of inflammatory bowel disease (IBD) is due in part to a loss of equilibrium among the gut microbiota, epithelial cells, and resident immune cells. The gut microbiota contains a large proportion of probiotic commensalLactobacillusspecies; some natural microbiota and probiotics confer protection against IBD. In this study, mice with colitis triggered by dextran sodium sulphate (DSS) were givenLactobacillus plantarumorally. We assessed the damage caused by DSS and the therapeutic activity ofL. plantarum. The colitis triggered by DSS was less severe in the mice that received theL. plantarumtreatment, which also diversified the microbe species in the colon, enhanced the ratio of Firmicutes to Bacteroidetes, and diminished the relative abundance ofLactobacillus. The taxonomic units of greatest diversity in the DSS andL. plantarumgroups were identified using a linear discriminant and effect size (LEfSe) analysis.Aliihoefleawas established to be the genus of bacteria that was affected in theL. plantarumgroup most extensively. In conclusion, gut health was promoted byL. plantarum, as it diversified the microbes in the colon and restricted the activity of pathogenic bacteria in the intestine. Moreover, according to the LEfSe analysis, the DSS group was impacted more significantly by gut microorganisms than theL. plantarumgroup, suggesting thatL. plantarumimproved the stability of the intestinal tract.

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“…Organisms of Sulfolobales are good models for studying CRISPR–Cas systems in Archaea, since a comprehensive array of genetic tools has been developed for several Sulfolobus species ( 58 ). In particular, seminar investigations of CRISPR–Cas mechanisms in S. solfataricus and S. islandicus have recently provided important insights into the adaptation process, the diverse modes of interference, and their modes of regulation ( 59 ).…”

Section: Discussionmentioning

confidence: 99%

Coupling transcriptional activation of CRISPR–Cas system and DNA repair genes by Csa3a in Sulfolobus islandicus

Liu

et al. 2017

Nucleic Acids Research

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CRISPR–Cas system provides the adaptive immunity against invading genetic elements in prokaryotes. Recently, we demonstrated that Csa3a regulator mediates spacer acquisition in Sulfolobus islandicus by activating the expression of Type I-A adaptation cas genes. However, links between the activation of spacer adaptation and CRISPR transcription/processing, and the requirement for DNA repair genes during spacer acquisition remained poorly understood. Here, we demonstrated that de novo spacer acquisition required Csa1, Cas1, Cas2 and Cas4 proteins of the Sulfolobus Type I-A system. Disruption of genes implicated in crRNA maturation or DNA interference led to a significant accumulation of acquired spacers, mainly derived from host genomic DNA. Transcriptome and proteome analyses showed that Csa3a activated expression of adaptation cas genes, CRISPR RNAs, and DNA repair genes, including herA helicase, nurA nuclease and DNA polymerase II genes. Importantly, Csa3a specifically bound the promoters of the above DNA repair genes, suggesting that they were directly activated by Csa3a for adaptation. The Csa3a regulator also specifically bound to the leader sequence to activate CRISPR transcription in vivo. Our data indicated that the Csa3a regulator couples transcriptional activation of the CRISPR–Cas system and DNA repair genes for spacer adaptation and efficient interference of invading genetic elements.

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Magnetic resonance imaging in personalized medicine

Cited by 15 publications

References 11 publications

Extraction, Characterization, Antioxidant, and Immunostimulatory Activities of Polysaccharides from Hedyotis corymbosa

Extraction, Characterization, Antioxidant, and Immunostimulatory Activities of Polysaccharides from Hedyotis corymbosa

The Impact ofLactobacillus plantarumon the Gut Microbiota of Mice with DSS-Induced Colitis

Coupling transcriptional activation of CRISPR–Cas system and DNA repair genes by Csa3a in Sulfolobus islandicus

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