Magnetically Multilayer Polysaccharide Membranes for Biomedical Applications

Gil, Sara; Silva, Joana M.; Mano, João F.

doi:10.1021/acsbiomaterials.5b00292

Cited by 24 publications

(26 citation statements)

References 60 publications

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“…In particular, Fe 3 O 4 nanoparticles have received enormous interests and attracted the most attention due to its unique magnetic properties and biocompatibility. Therefore, capsules and MNPs have been combined in several biomedical applications, including targeted drug delivery, TE, magnetic thermotherapy (hyperthermia), magnetic resonance imaging, among others . Previously, we immobilized rhodamine after a modification with a silane (APTES) at the surface of the proposed MNPs to monitor the cellular uptake and then further assemble cells in a sheet‐like 3D structure .…”

Section: Resultsmentioning

confidence: 99%

A Closed Chondromimetic Environment within Magnetic‐Responsive Liquified Capsules Encapsulating Stem Cells and Collagen II/TGF‐β3 Microparticles

Correia

Gil

Reis

et al. 2016

Adv Healthcare Materials

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TGF-β3 is enzymatically immobilized by transglutaminase-2 action to poly(l-lactic acid) microparticles coated with collagen II. Microparticles are then encapsulated with stem cells inside liquified spherical compartments enfolded with a permselective shell through layer-by-layer adsorption. Magnetic nanoparticles are electrostatically bound to the multilayered shell, conferring magnetic-response ability. The goal of this study is to engineer a closed environment inside which encapsulated stem cells would undergo a self-regulated chondrogenesis. To test this hypothesis, capsules are cultured in chondrogenic differentiation medium without TGF-β3. Their biological outcome is compared with capsules encapsulating microparticles without TGF-β3 immobilization and cultured in normal chondrogenic differentiation medium containing soluble TGF-β3. Glycosaminoglycans quantification demosntrates that similar chondrogenesis levels are achieved. Moreover, collagen fibrils resembling the native extracellular matrix of cartilage can be observed. Importantly, the genetic evaluation of characteristic cartilage markers confirms the successful chondrogenesis, while hypertrophic markers are downregulated. In summary, the engineered capsules are able to provide a suitable and stable chondrogenesis environment for stem cells without the need of TGF-β3 supplementation. This kind of self-regulated capsules with softness, robustness, and magnetic responsive characteristics is expected to provide injectability and in situ fixation, which is of great advantage for minimal invasive strategies to regenerate cartilage.

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Section: Resultsmentioning

confidence: 99%

A Closed Chondromimetic Environment within Magnetic‐Responsive Liquified Capsules Encapsulating Stem Cells and Collagen II/TGF‐β3 Microparticles

Correia

Gil

Reis

et al. 2016

Adv Healthcare Materials

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“…This can be overcome by the application of a mechanical stimulation based on a biomagnetic approach (Figure c). Different strategies based on biomagnetic biomaterial were developed, such as films, hydrogels, fibers, and 3D scaffolds . The combination of magnetic particles with polymeric biomaterials has demonstrated large potential for disease treatment and tissue repair, namely bone, muscle, nerve and cardiac tissue regeneration with external magnetic stimulation.…”

Section: Representative Biomedical Applicationsmentioning

confidence: 99%

Advances in Magnetic Nanoparticles for Biomedical Applications

Cardoso

Francesko

Ribeiro

et al. 2017

Adv Healthcare Materials

548

330

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Magnetic nanoparticles (NPs) are emerging as an important class of biomedical functional nanomaterials in areas such as hyperthermia, drug release, tissue engineering, theranostic, and lab-on-a-chip, due to their exclusive chemical and physical properties. Although some works can be found reviewing the main application of magnetic NPs in the area of biomedical engineering, recent and intense progress on magnetic nanoparticle research, from synthesis to surface functionalization strategies, demands for a work that includes, summarizes, and debates current directions and ongoing advancements in this research field. Thus, the present work addresses the structure, synthesis, properties, and the incorporation of magnetic NPs in nanocomposites, highlighting the most relevant effects of the synthesis on the magnetic and structural properties of the magnetic NPs and how these effects limit their utilization in the biomedical area. Furthermore, this review next focuses on the application of magnetic NPs on the biomedical field. Finally, a discussion of the main challenges and an outlook of the future developments in the use of magnetic NPs for advanced biomedical applications are critically provided.

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“…8 Very recently, it was shown that the incorporation of magnetic nanoparticles within freestanding Alg/Chi multilayer films gave rise to an increase in the elastic moduli measured in the wet state by stress−strain measurements. 12 Probe-based indentation is a versatile method to study local mechanical properties of materials from the nanoscale to the macroscale. 13−15 Within this method, nanoindenter and atomic force microscopy (AFM) are the most used techniques.…”

Section: ■ Introductionmentioning

confidence: 99%

Quantitative Nanomechanical Properties of Multilayer Films Made of Polysaccharides through Spray Assisted Layer-by-Layer Assembly

et al. 2016

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Nanomechanical properties of alginate/chitosan (Alg/Chi) multilayer films, obtained through spray assisted layer-by-layer assembly, were studied by means of PeakForce quantitative nanomechanical mapping atomic force microscopy (PF-QNM AFM). Prepared at two different alginate concentrations (1.0 and 2.5 mg/mL) and a fixed chitosan concentration (1.0 mg/mL), Alg/Chi films have an exponential growth in thickness with a transition to a linear growth toward a plateau by increasing the number of deposited bilayers. Height, elastic modulus, deformation, and adhesion maps were simultaneously recorded depending on the number of deposited bilayers. The elastic modulus of Alg/Chi films was found to be related to the mechanism of growth in contrast to the adhesion and deformation. A comparison of the nanomechanical properties obtained for non-cross-linked and thermally cross-linked Alg/Chi films revealed an increase of the elastic modulus after cross-linking regardless alginate concentration. The incorporation of iron oxide nanoparticles (NPs), during the spray preparation of the films, gave rise to nanocomposite Alg/Chi films with increased elastic moduli with the number of incorporated NPs layers. Deformation maps of the films strongly suggested the presence of empty spaces associated with the method of preparation. Finally, adhesion measurements point out to a significant role of NPs on the increase of the adhesion values found for nanocomposite films.

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Magnetically Multilayer Polysaccharide Membranes for Biomedical Applications

Cited by 24 publications

References 60 publications

A Closed Chondromimetic Environment within Magnetic‐Responsive Liquified Capsules Encapsulating Stem Cells and Collagen II/TGF‐β3 Microparticles

A Closed Chondromimetic Environment within Magnetic‐Responsive Liquified Capsules Encapsulating Stem Cells and Collagen II/TGF‐β3 Microparticles

Advances in Magnetic Nanoparticles for Biomedical Applications

Quantitative Nanomechanical Properties of Multilayer Films Made of Polysaccharides through Spray Assisted Layer-by-Layer Assembly

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