Magnetically separable polymer (Mag-MIP) for selective analysis of biotin in food samples

Abstract

This work presents an efficient method for the preparation of magnetic nanoparticles modified with molecularly imprinted polymers (Mag-MIP) through core-shell method for the determination of biotin in milk food samples. The functional monomer acrylic acid was selected from molecular modeling, EGDMA was used as cross-linking monomer and AIBN as radical initiator. The Mag-MIP and Mag-NIP were characterized by FTIR, magnetic hysteresis, XRD, SEM and N2-sorption measurements. The capacity of Mag-MIP for biotin adsorption, its kinetics and selectivity were studied in detail. The adsorption data was well described by Freundlich isotherm model with adsorption equilibrium constant (KF) of 1.46 mL g(-1). The selectivity experiments revealed that prepared Mag-MIP had higher selectivity toward biotin compared to other molecules with different chemical structure. The material was successfully applied for the determination of biotin in diverse milk samples using HPLC for quantification of the analyte, obtaining the mean value of 87.4% recovery.

Assessment of molecularly imprinted polymers (MIPs) in the preconcentration of disperse red 73 dye prior to photoelectrocatalytic treatment

“…3 were porous and homogeneous, exhibiting the "cauliflower clusters" appearance characteristic of other magnetic MIPs reported in the literature (Hussain et al 2016;Uzuriaga-Sánchez et al 2016). It can be seen from Fig.…”

“…In the next step, the Fe 3 O 4 @SiO 2 was modified with 3-methacryloxypropyltrimethoxysilane (MPS) by dispersing 250 mg of the Fe 3 O 4 @SiO 2 in 50 mL of anhydrous toluene containing 5 mL of MPS. The mixture was allowed to react for 12 h, under nitrogen bubbling, giving rise to the Fe 3 O 4 @SiO 2 -C=C material (Valero-Navarro et al 2011;Hussain et al 2016;Uzuriaga-Sánchez et al 2016). …”

“…The performance of the synthesized MIPs was evaluated using adsorption experiments and the construction of isotherms (Uzuriaga-Sánchez et al 2016). To this end, 40-mg portions of MMIP or MNIP were weighed out, separately, in 15-mL tubes, and conditioned by adding 4 mL of methanol and stirring for 3 h. The supernatant was then separated from the particles with the aid of a Nd magnet, followed by the addition of 4.0 mL of methanol to each tube containing the MMIPs and MNIPs, with stirring for a further 2 h in order to obtain homogeneous suspensions containing 10 mg mL −1 of the magnetic polymers.…”

A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format

“…There have been relatively few reports of the preparation of polymers imprinted with our chosen templates: fumonisin B2 MIPs for solid-phase extraction have been prepared by bulk polymerisation in acetonitrile8; glucosamine MIPs, prepared in aqueous buffer, have been reported for extraction of the monosaccharide from chicory root9; l -thyroxine-imprinted films have been prepared on electrodes for sensing applications1011; while biotin MIPs have been prepared as sol-gel magnetic core-shell particles12, as thin films1314 and in bulk format15. In contract to these diverse methods of synthesis, the imprinted polymer nanoparticles prepared in this work were all prepared in aqueous media, irrespective of the solubility or otherwise of the template molecules in water.…”

Monitoring the penetration and accumulation of gold nanoparticles in rat skin ex vivo using surface-enhanced Raman scattering spectroscopy

“…1 Recently, with the development of nanotechnology, nanomaterials are widely investigated and applied in many¯elds, such as biomedicine, cosmetics, catalysis, food, textiles, semiconductors, and biomedicines. [2][3][4][5] In addition to occupational exposure, direct human bodies exposures through commercial applications and ambient air pollution were a major concern.…”