Magnetite nanoparticles for functionalized textile dressing to prevent fungal biofilms development

Abstract

The purpose of this work was to investigate the potential of functionalized magnetite nanoparticles to improve the antibiofilm properties of textile dressing, tested in vitro against monospecific Candida albicans biofilms. Functionalized magnetite (Fe3O4/C18), with an average size not exceeding 20 nm, has been synthesized by precipitation of ferric and ferrous salts in aqueous solution of oleic acid (C18) and NaOH. Transmission electron microscopy, X-ray diffraction analysis, and differential thermal analysis coupled with thermo gravimetric analysis were used as characterization methods for the synthesized Fe3O4/C18. Scanning electron microscopy was used to study the architecture of the fungal biofilm developed on the functionalized textile dressing samples and culture-based methods for the quantitative assay of the biofilm-embedded yeast cells. The optimized textile dressing samples proved to be more resistant to C. albicans colonization, as compared to the uncoated ones; these functionalized surfaces-based approaches are very useful in the prevention of wound microbial contamination and subsequent biofilm development on viable tissues or implanted devices.

Impact of Biohybrid Magnetite Nanoparticles and Moroccan Propolis on Adherence of Methicillin Resistant Strains of Staphylococcus aureus

“…As reported by Mahdavi et al [27] for functionalized MNPs with oleic acid, XRD data showed negligible effects of this fatty acid on the XRD spectra data. The morphology and particle size of nanoparticles were similar to those reported by Anghel et al [15] for functionalized magnetite (Fe 3 O 4 /C 18 ), that is, the same type of functionalization (oleic acid) done in the present work. In the Mössbauer spectroscopy, and at room temperature, spectra showed a gradual passage from bulk-like to superparamagnetic (SPM) behavior, as the average size of the particles decrease, a striking characteristic of the superparamagnetic nature of nanoparticles [28].…”

“…Functionalized Fe 3 O 4 /C 18 nanoparticles without propolis extract present ability for inhibiting the adherence of MRSA strains, as already reported by Anghel et al [15], nevertheless always inferior when compared to those samples also functionalized with propolis extract, which reveals the importance of this extract on the activity.…”

“…In the Mössbauer spectroscopy, and at room temperature, spectra showed a gradual passage from bulk-like to superparamagnetic (SPM) behavior, as the average size of the particles decrease, a striking characteristic of the superparamagnetic nature of nanoparticles [28]. by Anghel et al [15] for functionalized magnetite (Fe3O4/C18), that is, the same type of functionalization (oleic acid) done in the present work. In the Mössbauer spectroscopy, and at room temperature, spectra showed a gradual passage from bulk-like to superparamagnetic (SPM) behavior, as the average size of the particles decrease, a striking characteristic of the superparamagnetic nature of nanoparticles [28].…”

“…In order to prevent that MNPs form aggregates, and for raising its biocompatibility, stability and biological application, such NPs may be functionalized with some biomolecules [14]. Oleic acid has been used alone or along with natural products for preventing biofilm formation of pathogenic microorganisms (C. albicans, C. tropicalis, S. aureus, and E. coli) or for promoting its disruption [12,15,16].…”

Antimicrobial Nanostructured Bioactive Coating Based on Fe3O4 and Patchouli Oil for Wound Dressing

“…Magnetite is one of the most used inorganic nanomaterials for variable biomedical, both clinical and research applications, including the development of novel antimicrobial strategies, due to their good biocompatibility [12] and intrinsic antimicrobial properties, as well as their quality to act in synergy with other antimicrobial drugs (such as antibiotics but also natural products that may have antimicrobial effects, such as plant-derived compounds and essential oils) or to act as effective drug carriers [13][14][15].…”