Magnetization transfer as a potential tool for the early detection of acute graft rejection after lung transplantation in mice

Kenkel, David; Yamada, Yoshito; Weiger, Markus; Jungraithmayr, Wolfgang; Wurnig, Moritz C.; Boss, Andreas

doi:10.1002/jmri.25266

Cited by 5 publications

(3 citation statements)

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“…These differences are likely caused by a higher protein content in the infiltrated lung tissue, which leads to a significantly higher MT due to the interaction of the macromolecular spin pool with the water spin pool. This observation is in line with findings in an acute rejection lung transplantation model [ 24 ], in which a higher MTR was observed in lung exhibiting acute rejection. Conversely, well-ventilated lung tissue showed a significantly lower MTR for nearly all flip angles and off-resonance frequencies compared to infiltrated lung except for an off-resonance frequency of 1000 Hz.…”

Section: Discussionsupporting

confidence: 92%

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Magnetisation transfer as a biomarker for chronic airway fibrosis in a mouse lung transplantation model

et al. 2018

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BackgroundChronic airway fibrosis (CAF) is the most prevalent complication in human lung transplant recipients. The aim of the study is to evaluate magnetisation transfer (MT) as a biomarker of developing CAF of lung transplants in a mouse model.MethodsLung transplantation was performed in 48 mice, applying major or minor histocompatibility mismatches between strains for the induction of CAF. MT measurements were performed in vivo with systematic variation of off-resonance frequencies and flip angle of the MT prepulse. MT ratios (MTRs) were compared for lungs showing CAF and without CAF.ResultsSeven out of 24 animals (29%) showed a pattern of CAF at histology. All mice developing CAF also showed signs of acute rejection, whereas none of the lungs showed signs of other post-transplant complications. After lung transplantation, pulmonary infiltration was a frequent finding (14 out of 24) exhibiting a higher MTR (24.8% ± 4.5%) compared to well-ventilated lungs (12.3% ± 6.9%, p = 0.001) at 8000 Hz off-resonance frequency, 3000° flip angle. In infiltrated lung tissue exhibiting CAF, lower MTR values (21.8% ± 5.7%) were found compared to infiltrated lungs showing signs of acute rejection alone (26.5% ± 2.9%, p = 0.028), at 8000 Hz, 3000° flip angle. The highest MTR values were observed at 3000° flip angle, using a 1000 Hz off-resonance frequency.ConclusionMTR might serve as a tool for the detection of CAF in infiltrated lung tissue.

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Section: Discussionsupporting

confidence: 92%

“…Third, in our animal model, all animals with CAF also showed acute rejection. However, the MTR differences between lungs with and without CAF were higher than in acute rejection alone, as previously published [ 24 ]. The findings are, however, not directly comparable due to the differences in the animal models.…”

Section: Discussionsupporting

confidence: 81%

Magnetisation transfer as a biomarker for chronic airway fibrosis in a mouse lung transplantation model

et al. 2018

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“…After heparinization, the inferior vena cava and left atrium are incised for exsanguination. Perfusion is performed with 10-20 mL 4 • C low-potassium dextran-glucose solution into the pulmonary artery (PA) at a constant pressure of 20-30 cm H 2 O (similar to the physiologic PA systolic pressure in rats) [20][21][22]. Perfusion needs to take place while the lungs are ventilated as this ensures flushing of the small capillaries.…”

Section: Rat Donor Organ Procurementmentioning

confidence: 99%

A Comprehensive Review on the Surgical Aspect of Lung Transplant Models in Mice and Rats

Jin

Kaes

Slambrouck

et al. 2022

Cells

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Lung transplantation improves the outcome and quality of life of patients with end-stage pulmonary disease. However, the procedure is still hampered by the lack of suitable donors, the complexity of the surgery, and the risk of developing chronic lung allograft dysfunction. Over the past decades, translational experiments in animal models have led to a better understanding of physiology and immunopathology following the lung transplant procedure. Small animal models (e.g., rats and mice) are mostly used in experiments regarding immunology and pathobiology and are preferred over large animal models due to the ethical aspects, the cost–benefit balance, and the high throughput possibility. In this comprehensive review, we summarize the reported surgical techniques for lung transplantation in rodent models and the management of perioperative complications. Furthermore, we propose a guide to help identify the appropriate species for a given experiment and discuss recent experimental findings in small animal lung transplant models.

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Short-T2 MRI: Principles and recent advances

Weiger

Pruessmann

2019

Progress in Nuclear Magnetic Resonance Spectroscopy

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Magnetization transfer as a potential tool for the early detection of acute graft rejection after lung transplantation in mice

Abstract: MT measurements may provide a tool for the quantitative assessment of AR. J. Magn. Reson. Imaging 2016;44:1091-1098.

Cited by 5 publications

References 29 publications

Magnetisation transfer as a biomarker for chronic airway fibrosis in a mouse lung transplantation model

Magnetisation transfer as a biomarker for chronic airway fibrosis in a mouse lung transplantation model

A Comprehensive Review on the Surgical Aspect of Lung Transplant Models in Mice and Rats

Short-T2 MRI: Principles and recent advances

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