Magnetoresponsive fluorescent core–shell nanoclusters for biomedical applications

Saladino, Giovanni Marco; Kakadiya, Ronak; Ansari, Shaquib Rahman; Teleki, Alexandra; Toprak, Muhammet S.

doi:10.1039/d2na00887d

Cited by 8 publications

(8 citation statements)

References 54 publications

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“…The colloidal stability of the Rh NPs in DMAc was demonstrated by the evaluation of the mean count rate (MCR) variation between three consecutive DLS measurements, yielding a relative standard deviation of 1.54%, ascribed to random colloidal fluctuations rather than precipitation. 38 Moreover, the obtained 0.32 ± 0.04 polydispersity index (PDI) further confirms the good dispersibility and narrow size distribution of the synthesized NPs in the given solvent, DMAc. The good dispersibility and colloidal stability were ascribed to the long-chain PVP (55 kDa) capping rather than NP morphology.…”

Section: Resultssupporting

confidence: 58%

“…When compared to the dry size, the obtained higher value for the colloidal size was ascribed to the additional contributions arising from the long-chain capping agent (PVP) and adsorbed solvent. The colloidal stability of the Rh NPs in DMAc was demonstrated by the evaluation of the mean count rate (MCR) variation between three consecutive DLS measurements, yielding a relative standard deviation of 1.54%, ascribed to random colloidal fluctuations rather than precipitation . Moreover, the obtained 0.32 ± 0.04 polydispersity index (PDI) further confirms the good dispersibility and narrow size distribution of the synthesized NPs in the given solvent, DMAc.…”

Section: Resultsmentioning

confidence: 63%

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Two-Photon Polymerization Printing with High Metal Nanoparticle Loading

Kilic,

Saladino,

Johansson

et al. 2023

ACS Appl. Mater. Interfaces

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Two-photon polymerization (2PP) is an efficient technique to achieve high-resolution, three-dimensional (3D)-printed complex structures. However, it is restricted to photocurable monomer combinations, thus presenting constraints when aiming at attaining functionally active resist formulations and structures. In this context, metal nanoparticle (NP) integration as an additive can enable functionality and pave the way to more dedicated applications. Challenges lay on the maximum NP concentrations that can be incorporated into photocurable resist formulations due to the lasertriggered interactions, which primarily originate from laser scattering and absorption, as well as the limited dispersibility threshold. In this study, we propose an approach to address these two constraints by integrating metallic Rh NPs formed ex situ, purposely designed for this scope. The absence of surface plasmon resonance (SPR) within the visible and near-infrared spectra, coupled with the limited absorption value measured at the laser operating wavelength (780 nm), significantly limits the laser-induced interactions. Moreover, the dispersibility threshold is increased by engineering the NP surface to be compatible with the photocurable resin, permitting us to achieve concentrations of up to 2 wt %, which, to our knowledge, is significantly higher than the previously reported limit (or threshold) for embedded metal NPs. Another distinctive advantage of employing Rh NPs is their role as promising contrast agents for X-ray fluorescence (XRF) bioimaging. We demonstrated the presence of Rh NPs within the whole 2PP-printed structure and emphasized the potential use of NP-loaded 3D-printed nanostructures for medical devices.

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Section: Resultssupporting

confidence: 58%

Section: Resultsmentioning

confidence: 63%

Two-Photon Polymerization Printing with High Metal Nanoparticle Loading

Kilic,

Saladino,

Johansson

et al. 2023

ACS Appl. Mater. Interfaces

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“…00-050-0739], like the as-synthesized MoO 2 NPs ( 18 , 24 ), demonstrating that the modified Stöber method did not affect the core NP structure. Ethanolamine (EA) permits a faster reaction rate, due to its strong catalytic effect ( 18 , 25 ), thus limiting the exposure of the core MoO 2 NPs. The silica formation was confirmed with Fourier transform infrared (FTIR) spectroscopy, where the Si─O─Si stretching vibration at 1079 cm −1 was evidenced (fig.…”

Section: Resultsmentioning

confidence: 99%

“…A modified Stöber method with EA as the base was used as previously reported in literature ( 18 , 25 ), with a few modifications. In a water/EtOH mix (1/3.75), MoO 2 NPs were dispersed (0.25 mg ml −1 in a total of 19 ml).…”

Section: Methodsmentioning

confidence: 99%

Iterative nanoparticle bioengineering enabled by x-ray fluorescence imaging

Saladino,

Brodin,

Kakadiya

et al. 2024

Sci. Adv.

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Nanoparticles (NPs) are currently developed for drug delivery and molecular imaging. However, they often get intercepted before reaching their target, leading to low targeting efficacy and signal-to-noise ratio. They tend to accumulate in organs like lungs, liver, kidneys, and spleen. The remedy is to iteratively engineer NP surface properties and administration strategies, presently a time-consuming process that includes organ dissection at different time points. To improve this, we propose a rapid iterative approach using whole-animal x-ray fluorescence (XRF) imaging to systematically evaluate NP distribution in vivo. We applied this method to molybdenum-based NPs and clodronate liposomes for tumor targeting with transient macrophage depletion, leading to reduced accumulations in lungs and liver and eventual tumor detection. XRF computed tomography (XFCT) provided 3D insight into NP distribution within the tumor. We validated the results using a multiscale imaging approach with dye-doped NPs and gene expression analysis for nanotoxicological profiling. XRF imaging holds potential for advancing therapeutics and diagnostics in preclinical pharmacokinetic studies.

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“…Magnetic nanoparticles (MNPs) are applied to an ever-broadening catalogue of theragnostic techniques [1], including magnetic hyperthermia therapy [2][3][4][5][6], field-guided therapies [7][8][9], biomarkers and cell/ protein separation [10,11], contrast-enhanced magnetic resonance imaging (MRI) [12][13][14][15][16] or magnetic particle imaging [17][18][19]. Progress in these applications depends on biocompatibility assays, which consider the actual number of nanoparticles internalized by the cells.…”

Section: Introductionmentioning

confidence: 99%

Toxicity and magnetometry evaluation of the uptake of core-shell maghemite-silica nanoparticles by neuroblastoma cells

López-Martín,

Aranda-Sobrino,

De Enciso-Campos

et al. 2024

R. Soc. Open Sci.

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Nanoparticle uptake by cells is a key parameter in their performance in biomedical applications. However, the use of quantitative, non-destructive techniques to obtain the amount of nanoparticles internalized by cells is still uncommon. We have studied the cellular uptake and the toxicity of core-shell maghemite-silica magnetic nanoparticles (MNPs), with a core diameter of 9 nm and a shell thickness of 3 nm. The internalization of the nanoparticles by mouse neuroblastoma 2a cells was evaluated by sensitive and non-destructive Superconducting Quantum Interference Device (SQUID) magnetometry and corroborated by graphite furnace atomic absorption spectroscopy. We were thus able to study the toxicity of the nanoparticles for well-quantified MNP uptake in terms of nanoparticle density within the cell. No significant variation in cell viability or growth rate was detected for any tested exposure. Yet, an increase in both the amount of mitochondrial superoxide and in the lysosomal activity was detected for the highest concentration (100 μg ml −1 ) and incubation time (24 h), suggesting the onset of a disruption in ROS homeostasis, which may lead to an impairment in antioxidant responses. Our results validate SQUID magnetometry as a sensitive technique to quantify MNP uptake and demonstrate the non-toxic nature of these core-shell MNPs under our culture conditions.

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Magnetoresponsive fluorescent core–shell nanoclusters for biomedical applications

Abstract: Nowadays, superparamagnetic iron oxide nanoparticles (SPIONs) have a dominant role in many subfields of biomedicine. Owing to their peculiar properties, they can be employed for magnetic separation, drug delivery, diagnostics,...

Cited by 8 publications

References 54 publications

Two-Photon Polymerization Printing with High Metal Nanoparticle Loading

Two-Photon Polymerization Printing with High Metal Nanoparticle Loading

Iterative nanoparticle bioengineering enabled by x-ray fluorescence imaging

Toxicity and magnetometry evaluation of the uptake of core-shell maghemite-silica nanoparticles by neuroblastoma cells

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