Magnifection?a new platform for expressing recombinant vaccines in plants

Gleba, Yuri; Klimyuk, Victor; Marillonnet, Sylvestre

doi:10.1016/j.vaccine.2005.01.006

Cited by 384 publications

(285 citation statements)

References 14 publications

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“…Deleted: [72] Deleted: top Deleted: [73] Deleted: [74] Deleted: based in 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 ensures cell-to-cell spreading of the replicon throughout the entire agroinfiltrated leaf [79,80]. Since RNA viruses do not enter the nucleus in its natural life cycle, Icon's vector was elegantly tailored for its artificial nucleus-to cytoplasm passage by addition of introns and removal of cryptic splicing sites [81].…”

Section: Deleted: Alsomentioning

confidence: 99%

Manufacturing antibodies in the plant cell

Orzáez

Granell

Blázquez

2009

Biotechnology Journal

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International audiencePlants have long been considered advantageous platforms for large-scale production of antibodies due to their low cost, scalability, and the low chances of pathogen contamination. Much effort has therefore been devoted to efficiently produce mAbs (from nanobodies to secretory antibodies) in plant cells. Several technical difficulties have been encountered and are being coped with. Improvements in production levels have been achieved by manipulation of gene expression and, more efficiently, of cell targeting and protein folding and assembly. Differences in mAb glycosylation patterns between animal and plant cells are being successfully addressed by the elimination and introduction of the appropriate enzyme activities in plant cells. Another relevant battlefield is the dichotomy between production capacity and speed. Classically, stably transformed plant lines have been proposed for large scale mAb production, whereas the use of transient expression systems has always provided production speed at the cost of scalability. However, recent advances in transient expression techniques have brought impressive yield improvements, turning speed and scalability highly compatible assets. In the era of personalized medicines, the combination of yield and speed, and the advances in glyco-engineering have made the plant cell a serious contender in the field of recombinant antibody production

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Section: Deleted: Alsomentioning

confidence: 99%

Manufacturing antibodies in the plant cell

Orzáez

Granell

Blázquez

2009

Biotechnology Journal

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“…Magnifection, for example, has been shown to increase transfection efficiency enormously and does not require systemic movement of the virus to achieve optimal results. This procedure takes place by the immersion of an entire plant into a suspension of Agrobacterium and application of a weak vacuum, followed by a gentle return to atmospheric pressure Marillonnet et al 2005). This results in an infiltration of the Agrobacterium suspension containing the construct(s) of interest into the intercellular space of all mature leaves of the plant host.…”

Section: Discussionmentioning

confidence: 99%

“…This results in the release of replicons throughout the host from a chromosomally encoded pro-replicon or pro-virus, thus eliminating the requirement for cell-to-cell spread of the expression vector (Hefferon, 2006). This 'deconstructed virus strategy' has been examined in a novel TMV-based vector system in which various models of the viral vector were differentially transformed into Agrobacteria and delivered into plants, where the modules could be reassembled and the gene of interest expressed at extremely high yields (Gleba et al 2004;Gleba et al 2005;Marillonnet et al 2005). …”

Section: *Corresponding Authormentioning

confidence: 99%

Amplification of genome-integrated BeYDV replicons by transient expression of Rep in Arabidopsis thaliana

Yong-Sun¹,

Hefferon²

2007

Electron. J. Biotechnol.

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“…Standard abrasion methods are too tedious to deliver inoculum to each leaf, so new methods were developed. The most common method is Agro-infiltration of host plants to launch the infection process [33,[52][53][54]. This process introduces a DNA plasmid, containing the virus vector under the control of an appropriate transcriptional unit within normal Ti plasmid integration sites, into Agrobacterium tumefaciens cells to create an inoculum.…”

Section: Minimal-virus Vectorsmentioning

confidence: 99%