Magnolol pretreatment attenuates heat stress-induced IEC-6 cell injury

Chen, Mei; He, Shasha; Yin, Peng; Xu, Lei; Shi, Yaran; Yu, Xin; Lyu, An; Li, Fenghua; Jiang, Linshu

doi:10.1631/jzus.b1500261

Cited by 7 publications

(7 citation statements)

References 44 publications

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“…In our study, we found that MA significantly enhanced ileal villus height and duodenal V/C ratio. Mei et al. (2016) also observed that magnolol pretreatment attenuates heat stress-induced injury in intestinal epithelial cell-6 (IEC-6) and then maintained the intact structures and functions of the small intestine.…”

Section: Discussionmentioning

confidence: 86%

“…Magnolol was known as a natural polyphenolic compound and was found to have antioxidant activity ( Shen et al., 2010 ). The antioxidant activity of magnolol was observed to protect the structure and functions of organs ( Kim et al., 2013 , Mei et al., 2016 , Shen et al., 2010 , Zhao and Liu, 2011 ). These data suggest that magnolol may be a potential candidate to improve growth performance of animals.…”

Section: Discussionmentioning

confidence: 99%

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Magnolol additive as a replacer of antibiotic enhances the growth performance of Linwu ducks

et al. 2017

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Magnolol rich in Magnolia officinalis is a bioactive polyphenolic compound. The aim of this study was to examine the effects of magnolol additive (MA) on growth performance, expression levels of antioxidant-related genes, and intestinal mucosal morphology of Linwu ducks aged from 49 to 70 days, comparing with that of an antibiotic additive (colistin sulfate [CS]). A total of 275, 49-day-old ducks were assigned to 5 groups with 5 cages of 11 ducks each and fed diets supplemented with 0, 100, 200 and 300 mg of MA/kg and 300 mg of CS/kg for 3 weeks, respectively. The results showed that the average daily body weight gain (ADG) was increased significantly in MA-fed groups (200 and 300 mg/kg), compared with the basal diet (BD) group (P < 0.05). The mRNA levels of superoxide dismutase-1 (SOD1), manganese superoxide dismutase-2 (MnSOD2) and catalase (CAT) were also increased significantly in MA groups (P < 0.05). In addition, hematoxylin and eosin staining revealed that Linwu ducks fed the diets with MA had more intact intestinal mucosa than those fed the BD and CS diets. In addition, ileal villus height, ileal villus height/crypt depth ratio (V/C) and duodenal V/C were also improved significantly (P < 0.05). Taken together, these data demonstrated that MA is an effective feed additive to enhance the growth performance of the Linwu ducks by improving the antioxidant and intestinal mucosal status, suggesting that MA will be a potential additive to replace antibiotic (CS).

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Magnolol additive as a replacer of antibiotic enhances the growth performance of Linwu ducks

et al. 2017

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“…However, these functions can be compromised by heat stroke [ 25 , 26 ]. Reduced blood flow to the small intestine following heat stroke occurs to preserve flow to essential organs, such as the brain and the heart [ 5 , 12 , 13 ]. This process greatly impairs the epithelial cells of the small intestinal villi, induces excessive apoptosis of epithelial cells, and leads to an increased risk of morbidity and mortality [ 12 , 13 ].…”

Section: Discussionmentioning

confidence: 99%

“…The increase in free radicals from ischemia can lead to ROS-related damage of the intestinal mucosa. Heat stress-related oxidative stress has been shown to cause apoptosis in the small intestines of both rats and pigs [ 5 , 13 , 14 ]. Therefore, oxidative stress may be the intermediate through which heat stress induces intestinal damage and may act upstream of apoptosis.…”

Section: Introductionmentioning

confidence: 99%

Heat stress induces intestinal injury through lysosome- and mitochondria-dependent pathwayin vivoandin vitro

Gao

Luo

et al. 2017

Oncotarget

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Damage to the small intestine secondary to heat stroke is a major factor in heat stroke-related morbidity and mortality. However, the underlying mechanisms by which heat stroke causes small intestinal lesions and dysfunction remain unclear. To explore the pathogenesis of small intestinal tissue and epithelial cell injury, the SW480 cell heat stress model and the mice heat stroke model were established to mimic heat stroke. Morphologic changes in intestinal tissue and increased TUNEL-positive index were induced by heat stress in vivo. Heat stress activated the lysosomal-mitochondrial apoptotic pathway in SW480 cells, increasing intracellular reactive oxygen species and causing lysosomal membrane permeabilization with subsequent release of cathepsin B to the cytosol, mitochondrial depolarization, and cytochrome C release to cytosol. An increase in the Bax/Bcl2 ratio, caspase-9 and caspase-3 were observed. N-Acetyl-L-Cysteine was shown to inhibit ROS generation, suppress permeabilization of lysosomal membranes, decrease levels of cathepsin B and cytochrome C in the cytosol, and inhibit Bax/Bcl2 ratio, caspase-9 and caspase-3 activity both in vitro and in vivo. Mitochondrial damage was alleviated when the models were pre-treated with CA-074 Me both in vitro and in vivo, decreasing cathepsin B and cytochrome C levels in the cytosol, Bax/Bcl2 ratio, caspase-9 and caspase-3 activity. In our models, heat stress-induced apoptosis of small intestinal tissue and epithelial cells through accumulation of ROS and activation of the lysosomal–mitochondrial apoptotic pathway involved the release of cathepsin B. These findings may offer potentially pharmaceutical targets and strategies to repair intestinal injury caused by heat stroke.

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“…It has been reported that HS causes intestinal epithelial cell line (IEC-6) injury and cell cycle arrest at G1 phase, as indicated by increased expression of p21 and p27 and decreased expression of E2F1, pRb, cyclin D1, and CDK4. Magnolol pretreatment protects against HS-triggered injury in IEC-6 cells effectively [113]. Magnolol has been demonstrated to show antiphotoaging activity in UV-irradiated hairless mice, as evidenced by reduction of the average length and depth of wrinkles, downregulation of MMP-1, MMP-9, and MMP-13 expression, and attenuation of MAPKs phosphorylation, including p38, ERK1/2, and JNK [114].…”

Section: Clinical Prospectivementioning

confidence: 99%

Insights on the Multifunctional Activities of Magnolol

Zhang

Chen

Huang

et al. 2019

BioMed Research International

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Over years, various biological constituents are isolated from Traditional Chinese Medicine and confirmed to show multifunctional activities. Magnolol, a hydroxylated biphenyl natural compound isolated from Magnolia officinalis, has been extensively documented and shows a range of biological activities. Many signaling pathways include, but are not limited to, NF-κB/MAPK, Nrf2/HO-1, and PI3K/Akt pathways, which are implicated in the biological functions mediated by magnolol. Thus, magnolol is considered as a promising therapeutic agent for clinic research. However, the low water solubility, the low bioavailability, and the rapid metabolism of magnolol dramatically limit its clinical application. In this review, we will comprehensively discuss the last five-year progress of the biological activities of magnolol, including anti-inflammatory, antimicroorganism, antioxidative, anticancer, neuroprotective, cardiovascular protection, metabolism regulation, and ion-mediating activity.

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Magnolol pretreatment attenuates heat stress-induced IEC-6 cell injury

Abstract: Magnolol pretreatment attenuates HS-induced injury in IEC-6 cells. Magnolol is potentially promising as a protective strategy for HS in livestock.

Cited by 7 publications

References 44 publications

Magnolol additive as a replacer of antibiotic enhances the growth performance of Linwu ducks

Magnolol additive as a replacer of antibiotic enhances the growth performance of Linwu ducks

Heat stress induces intestinal injury through lysosome- and mitochondria-dependent pathwayin vivoandin vitro

Insights on the Multifunctional Activities of Magnolol

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