Main flavonoids in the root of Scutellaria baicalensis cultivated in Europe and their comparative antiradical properties

Abstract

The content of the main flavonoids in the root of Scutellaria baicalensis Georgi cultivated in Central Europe was evaluated using the new simple RP-HPLC method with gradient of acetonitrile in mobile phase. The main components of the roots were baicalin (8.12% of dry root mass) and wogonin glucuronide (2.52%). The content of flavonoids was comparable with the content in plants cultivated in natural localities. Five main flavonoids were evaluated for their scavenging ability with DPPH radical-generating system and due to limited solubility only two flavonoids were investigated for their ability to scavenge hydroxyl radical by the aromatic hydroxylation method. The total extract was also tested in both the experimental arrangements. In experiments with DPPH, only baicalin and baicalein displayed a significant scavenging effect, while the production of OH radicals generated by UV photolysis of H(2)O(2) was considerable decreased in the presence of baicalin and wogonin glucuronide. After comparison with results obtained for the total extract, it was concluded, that the scavenging activity of the extract against DPPH is mainly derived from baicalin. On the other hand, baicalin, wogonin glucuronide and probably other flavonoids participate in scavenging OH radical.

Suppressive Mechanisms of Sairei-to on Mesangial Matrix Expansion in Rat Mesangioproliferative Glomerulonephritis

“…A previous study showed that a scavenger for ROS suppressed urinary protein and the proliferation of mesangial cells in thy-1 nephritis [27]. Since flavonoids in the root of Scutellaria baicalensis cultivated in Europe, which a common herb in TJ-9, TJ-96, and TJ-114, which have anti-oxidant activity [28], it is suggested that these flavonoids, including baicalin, oroxylin A, and woogonin in figure 1, suppressed this experimental nephritis by their anti-oxidative action. To evaluate the oxidative status in the glomeruli, SOD-activity of a renal cortex homogenate was measured.…”

Performance and selected blood parameters of broiler chickens fed diets with skullcap (<i>Scutellaria baicalensis</i> Georgi) root

“…It has a particularly high content of flavonoids, which are modifiers of inflammatory processes, prevent bacterial infections and have antiviral, antitumor and antioxidative properties as well as hepatoprotecive effects (Gao et al, 1999;Chan et. al., 2000;Bochorakova et al, 2003). The objective of this study was to evaluate the effect of SCR on the performance of broiler chickens and selected blood parameters.…”

Preventive Effects of Chinese Herb Chai-Hu-Gui-Zhi-Tang Extract on Water Immersion Restraint Stress-Induced Acute Gastric Ulceration in Rats

“…Among the herbal drugs contained in GHGZT, Scutellariae radix [6] and Panax ginseng [11] have been used extensively, and their clinical efficacies have been documented in regard to anti-ulcer activity. The flavonoids of Scutellariae radix have been found to be free radical scavengers [3]; free radicals play an important role in ulcerative and erosive lesions of the gastrointestinal tract. Glycyrrhizae radix water extract and its two major constituents, glycyrrhizin and 3-glycyrrhetinic monodesmoside, may be promising for amelioration of hypoxia (ischemia)-reoxygenation (reperfusion) injury and improvement of renal function by acting as antioxidant and oxygen radicalscavenging agents [32].…”