MAINTAINERS AND RESTORERS FOR THREE MALE-STERILITY-INDUCING CYTOPLASMS IN RAPE (<i>Brassica napus</i> L.)

Fan, Zhegong; Stefansson, B. R.; Sernyk, J. L.

doi:10.4141/cjps86-036

Cited by 37 publications

(12 citation statements)

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“…The male fertile ''maintainer'' strains of these varieties contain the fertile cam cytoplasm derived from the related species Brassica campestris (13,16). Most B. napus varieties lack a restorer gene for pol CMS and, hence, are sterilized by pol cytoplasm (14). Restorer genes for pol CMS have been identified in various strains (14,17) and map to a single nuclear locus designated Rfp (18).…”

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confidence: 99%

“…Most B. napus varieties lack a restorer gene for pol CMS and, hence, are sterilized by pol cytoplasm (14). Restorer genes for pol CMS have been identified in various strains (14,17) and map to a single nuclear locus designated Rfp (18). The relationship between the various B. napus cytoplasms, restorer genes, and male sterility or fertility are outlined in Table 1.…”

mentioning

confidence: 99%

“…Most B. napus varieties contain the nap cytoplasm but are male-fertile because they possess a restorer gene for nap CMS, designated here as Rfn (14). The nap cytoplasm confers male sterility on a few exceptional varieties that lack Rfn, such as the cultivar ''Bronowski'' (13,15).…”

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confidence: 99%

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Restorer genes for different forms of Brassica cytoplasmic male sterility map to a single nuclear locus that modifies transcripts of several mitochondrial genes

Jean

Landry

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

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The oilseed rape plant, Brassica napus, possesses two endogenous male sterile cytoplasms, nap and pol. Previous studies have shown that nuclear restoration of pol cytoplasmic male sterility (CMS) is conditioned by a gene, Rfp, that is also involved in modifying transcripts of the pol CMS-associated orf224͞atp6 mtDNA region. We now find that the nap nuclear restorer gene Rfn apparently is identical to Mmt, a gene that conditions the modification of transcripts from several different mtDNA regions, including one that is associated with nap CMS and contains orf222, a chimeric gene related to orf224. Mmt, in turn, is found to be allelic to Rfp, suggesting that restorer genes for the two cytoplasms represent different alleles or haplotypes of a single nuclear locus. This view is supported by restriction fragment length polymorphism mapping studies that indicate that

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Restorer genes for different forms of Brassica cytoplasmic male sterility map to a single nuclear locus that modifies transcripts of several mitochondrial genes

Jean

Landry

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

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“…For that reason the male fertility/sterility character of interspecific hybrids and their consecutive BCs was determined by the maternal component. The CMS-Ogura retained all of its characteristics in the consecutive F 1 -BC 3 generations, irrespective of different environmental conditions and genetic backgrounds, with normal floral development in contrast with the pol CMS described by Fan et al (1986). For plant breeders such stable expression of the maternally inherited male sterility through F 1 -BC 3 generations should be sufficient proof for the presence of Ogu-INRA cytoplasm.…”

Section: Discussionmentioning

confidence: 91%

Interspecific hybridisation of cytoplasmic male-sterile rapeseed with Ogura cytoplasm and Brassica rapa var. pekinensis as a method to obtain male-sterile Chinese cabbage inbred lines

et al. 2015

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During 2011-2014, three self-compatible Chinese cabbage inbred lines (Brassica rapa, genome AA 2n = 20) were sexually hybridised with cytoplasmic male-sterile (CMS Ogu-INRA) and fertile lines of rapeseed (Brassica napus var. oleifera, genome AACC 2n = 38). Interspecific F 1 hybrids and their consecutive back-crosses (BCs) toward B. rapa were obtained, and their morphological and commercial characteristics, as well as their ability for generative propagation, were analysed. Cytometric and morphological analyses confirmed the interspecific character of the F 1 hybrids in comparison with B. napus and B. rapa parental lines. All interspecific F 1 hybrids had intermediate morphological characteristics typical of both parental components in the generative and vegetative stages. The F 1 hybrids of CMS B. napus 9 B. rapa were sterile, as were those of fertile B. napus 9 B. rapa. Good seed productivity from CMS B. napus 9 B. rapa and consecutive back-cross 1 (BC 1 )-BC 3 generations with Chinese cabbage as the recurrent parent was obtained. Plants of the BC 1 generation of CMS B. napus 9 B. rapa were sterile and had highly diversified morphological characteristics, both in the vegetative and generative phases. The BC 3 generation of CMS B. rapa was also sterile and more uniform than BC 1 , with good vigour and most of the typical Chinese cabbage agronomic traits. As a result, a collection of Chinese cabbage genotypes having the CMS trait, which is valuable for the breeding of commercial F 1 hybrids, was obtained for the first time in Poland.

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“…Several CMS (cytoplasmic MS) systems have been reported in many crops and have been extensively utilized for hybrid seed production, such as in rich (Li & Yuan, 2000;Virmani, 2003) and rapeseed (Fan et al, 1986;Röbbelen, 1991). The utilization of heterosis in common wheat was made possible by the establishment of the nuclear-cytoplasm interactive male sterility in the three-line system and by the use of chemical hybridizing agents (CHA) (Zhao et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Proteomics Analysis of Photo-Thermo-Sensitive Male Sterility Wheat Line BNS during Its Thermosensitive Period

Wang¹,

Shen²,

Wang³

et al. 2017

JAS

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Photo-thermo sensitive male sterile (PTMS) line is one of the important materials in utilizing heterosis in crops. Wheat line BNS (Bainong sterility) is an important nuclear-controlling PTMS line and suitable for growing and seed production in Huang Huai wheat zone in China. It has genetic stability with male sterility when sowing in autumn and male fertility when sowing in spring. Their thermosensitive periods were between stamen and pistil differentiation stage and anther connective stage and they could be regarded key periods for fertility conversion in BNS. To determine the molecular mechanisms of fertility conversion at thermosensitive period, we investigated characters of seed setting, anther and pollen grain of fertile and sterile BNS plants and compared young spike proteome patterns at their thermosensitive periods between the two BNS plants. The results showed that sterile plants had lower seed setting rate and pollen number, small pollen grain and lower pollen vitality than fertile plants. Out of protein spots reproducibly detected and analyzed on two dimensional electrophoresis gels, 76 spots showed significant changes in at least one BNS plant and 36 spots were identified by MALDI-TOF MS. The results showed that proteins involved in multiple biochemical pathways were differentially expressed at thermosensitive period between the two plants, including energy metabolism, stress response, signal transduction and regulation, protein process, amino acid and fatty acid metabolism, nucleic acid metabolism etc. Some of these proteins are reported to be involved in the abortion of anther or pollen grains in MS plants, such as energy metabolism and anti oxidative stress, and some were found to be novel proteins involved in the fertility conversion, such as phytohormones regulation. These results indicated that proteins related with anther or pollen development had expressed differently between the two BNS plants before anther development and phytohormones and signal transduction might be involved in the regulation of fertility conversion at thermosensitive period. Our studies have provided new insight to reveal the molecular mechanisms of fertility conversion at thermosensitive period in PTMS wheat.

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MAINTAINERS AND RESTORERS FOR THREE MALE-STERILITY-INDUCING CYTOPLASMS IN RAPE (Brassica napus L.)

Cited by 37 publications

References 1 publication

Restorer genes for different forms of Brassica cytoplasmic male sterility map to a single nuclear locus that modifies transcripts of several mitochondrial genes

Restorer genes for different forms of Brassica cytoplasmic male sterility map to a single nuclear locus that modifies transcripts of several mitochondrial genes

Interspecific hybridisation of cytoplasmic male-sterile rapeseed with Ogura cytoplasm and Brassica rapa var. pekinensis as a method to obtain male-sterile Chinese cabbage inbred lines

Proteomics Analysis of Photo-Thermo-Sensitive Male Sterility Wheat Line BNS during Its Thermosensitive Period

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