Maintenance of Multipotent Mesenchymal Stem Cells of Farm Animals in Cryogels Based on Naturally-Derived Polymers

Abstract

* Работа выполнена в рамках НИР ¹ 0578-2018-0006 «Создание новых клеточных систем с заданными свойствами на основе стволовых клеток млекопитающих, в том числе сельскохозяйственных животных для ветеринарии, вирусологии и биотехнологии».

Cryostructuring of Polymeric Systems. 55. Retrospective View on the More than 40 Years of Studies Performed in the A.N.Nesmeyanov Institute of Organoelement Compounds with Respect of the Cryostructuring Processes in Polymeric Systems

“…are of significance. Both manual measurements and computer-assisted approaches have been used for the mathematical treatment of the images obtained with the aid of various types of microscopy (optical [ 170 , 171 , 172 , 206 , 225 , 230 , 231 , 232 , 233 , 234 , 237 , 238 , 240 , 241 , 243 , 244 , 245 , 246 , 250 , 251 , 252 , 256 , 257 , 258 , 259 , 261 , 262 , 263 , 264 , 265 , 267 , 268 , 269 , 270 , 272 , 380 , 383 , 384 , 390 , 393 ], laser confocal [ 369 , 370 , 371 , 372 , 392 ], and SEM [ 173 , 174 , 177 , 179 , 181 , 182 , 184 , 196 , …”

“…The latter problem has been shown to be solved in the case of the gelatin scaffolds by performing the cryostructuring process in the DMSO/gelatin solutions followed by subsequent treatments performed in the ethanol medium ( 65 in Table 2 ). Such procedure resulted in the sterile wide-pore scaffolds ( Figure 1 I) suitable for the 3D culture and transformation of stem cells [ 382 , 383 , 384 ].…”