Maize E2F transcription factors. Expression, association to promoters of S-phase genes and interaction with the RBR1 protein in chromatin during seed germination

Sánchez-Camargo, Víctor A.; Suárez-Espinoza, Cassandra; Romero-Rodríguez, Samantha; Garza-Aguilar, Sara M.; Stam, Maike; García-Ramírez, Elpidio; Lara‐Núñez, Aurora; Vázquez‐Ramos, Jorge M.

doi:10.1016/j.plantsci.2020.110491

Cited by 7 publications

(3 citation statements)

References 75 publications

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“…A similar phenomenon was consistently observed in the PheE2F/DP gene family [ 25 ]. A previous study showed that there are 12 ZmE2F genes in maize [ 51 ], which is much lower than the number identified in this study. This may be due to their preliminary BLAST search using maize genome release 5b.60 and not a comprehensive analysis [ 51 ].…”

Section: Discussioncontrasting

confidence: 81%

Comprehensive identification of maize ZmE2F transcription factors and the positive role of ZmE2F6 in response to drought stress

Cao,

Wang,

et al. 2024

BMC Genomics

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Background The early 2 factor (E2F) family is characterized as a kind of transcription factor that plays an important role in cell division, DNA damage repair, and cell size regulation. However, its stress response has not been well revealed. Results In this study, ZmE2F members were comprehensively identified in the maize genome, and 21 ZmE2F genes were identified, including eight E2F subclade members, seven DEL subfamily genes, and six DP genes. All ZmE2F proteins possessed the DNA-binding domain (DBD) characterized by conserved motif 1 with the RRIYD sequence. The ZmE2F genes were unevenly distributed on eight maize chromosomes, showed diversity in gene structure, expanded by gene duplication, and contained abundant stress-responsive elements in their promoter regions. Subsequently, the ZmE2F6 gene was cloned and functionally verified in drought response. The results showed that the ZmE2F6 protein interacted with ZmPP2C26, localized in the nucleus, and responded to drought treatment. The overexpression of ZmE2F6 enhanced drought tolerance in transgenic Arabidopsis with longer root length, higher survival rate, and biomass by upregulating stress-related gene transcription. Conclusions This study provides novel insights into a greater understanding and functional study of the E2F family in the stress response.

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Section: Discussioncontrasting

confidence: 81%

Comprehensive identification of maize ZmE2F transcription factors and the positive role of ZmE2F6 in response to drought stress

Cao,

Wang,

et al. 2024

BMC Genomics

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“…This transition has been well studied during maize germination, where changes in the levels of a complex set of D‐type cyclins and KRPs are crucial to control CDK activity (Garza‐Aguilar et al , ; Godínez‐Palma et al , ). D‐type cyclins directly affect RBR1 phosphorylation levels and the release of E2F activity on target genes required to establish active cell proliferation and to initiate vegetative growth (Sánchez‐Camargo et al , ).…”

Section: Introductionmentioning

confidence: 99%

Roles of plant retinoblastoma protein: cell cycle and beyond

Desvoyes

Gutiérrez

2020

The EMBO Journal

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The human retinoblastoma (RB1) protein is a tumor suppressor that negatively regulates cell cycle progression through its interaction with members of the E2F/DP family of transcription factors. However, RB-related (RBR) proteins are an early acquisition during eukaryote evolution present in plant lineages, including unicellular algae, ancient plants (ferns, lycophytes, liverworts, mosses), gymnosperms, and angiosperms. The main RBR protein domains and interactions with E2Fs are conserved in all eukaryotes and not only regulate the G1/S transition but also the G2/M transition, as part of DREAM complexes. RBR proteins are also important for asymmetric cell division, stem cell maintenance, and the DNA damage response (DDR). RBR proteins play crucial roles at every developmental phase transition, in association with chromatin factors, as well as during the reproductive phase during female and male gametes production and embryo development. Here, we review the processes where plant RBR proteins play a role and discuss possible avenues of research to obtain a full picture of the multifunctional roles of RBR for plant life.

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“…In contrast, to our results E2F/DP proteins were expressed in the nucleus and cytoplasm in wheat [ 20 ]. These results suggested that SlE2F/DP3 may act as a transcription repressor and that other E2F/DP proteins may act as co-activators which is required for E2F activity or in regulating signalling pathways [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide characterization and expression profiling of E2F/DP gene family members in response to abiotic stress in tomato (Solanum lycopersicum L.)

Divya,

Robin,

Cho

et al. 2024

BMC Plant Biol

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Background E2F/DP (Eukaryotic 2 transcription factor/dimerization partner) family proteins play an essential function in the cell cycle development of higher organisms. E2F/DP family genes have been reported only in a few plant species. However, comprehensive genome-wide characterization analysis of the E2F/DP gene family of Solanum lycopersicum has not been reported so far. Results This study identified eight nonredundant SlE2F/DP genes that were classified into seven groups in the phylogenetic analysis. All eight genes had a single E2F-TDP domain and few genes had additional domains. Two segmental duplication gene pairs were observed within tomato, in addition to cis-regulatory elements, miRNA target sites and phosphorylation sites which play an important role in plant development and stress response in tomato. To explore the three-dimensional (3D) models and gene ontology (GO) annotations of SlE2F/DP proteins, we pointed to their putative transporter activity and their interaction with several putative ligands. The localization of SlE2F/DP-GFP fused proteins in the nucleus and endoplasmic reticulum suggested that they may act in other biological functions. Expression studies revealed the differential expression pattern of most of the SlE2F/DP genes in various organs. Moreover, the expression of E2F/DP genes against abiotic stress, particularly SlE2F/DP2 and/or SlE2F/DP7, was upregulated in response to heat, salt, cold and ABA treatment. Furthermore, the co-expression analysis of SlE2F/DP genes with multiple metabolic pathways was co-expressed with defence genes, transcription factors and so on, suggested their crucial role in various biological processes. Conclusions Overall, our findings provide a way to understand the structure and function of SlE2F/DP genes; it might be helpful to improve fruit development and tolerance against abiotic stress through marker-assisted selection or transgenic approaches.

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Maize E2F transcription factors. Expression, association to promoters of S-phase genes and interaction with the RBR1 protein in chromatin during seed germination

Cited by 7 publications

References 75 publications

Comprehensive identification of maize ZmE2F transcription factors and the positive role of ZmE2F6 in response to drought stress

Comprehensive identification of maize ZmE2F transcription factors and the positive role of ZmE2F6 in response to drought stress

Roles of plant retinoblastoma protein: cell cycle and beyond

Genome-wide characterization and expression profiling of E2F/DP gene family members in response to abiotic stress in tomato (Solanum lycopersicum L.)

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