The effects of seed inoculation with Rhizobium and inorganic nitrogen fertilization on some physiological and agronomical traits of chickpea (Cicer arietinum L.) cv. ILC 482, were investigated at the Experimental Farm of the Agriculture Faculty, University of Mohaghegh Ardabili. The trial was laid out in split plot design based on randomized complete block with four replications. Experimental factors were mineral nitrogen fertilizer at four levels (0, 50, 75 and 100 kg urea ha -1 ) in the main plots, and two levels of inoculation with Rhizobium bacteria (with and without inoculation) as sub plots. N application and Rh. inoculation showed positive effects on physiological and agronomical traits of chickpea. The highest value of leaf RWC was recorded at 50 kg urea ha -1 what was statistically in par with 75 kg urea ha -1 application, while the usage of 75 kg urea ha -1 showed the maximum stem RWC. The maximum CMS was obtained from application of 75 kg urea ha ) and Rh. inoculation. Application of 75 kg urea ha -1 was statistically in par with 100 kg urea ha -1 in all of these traits. The results pointed out that some N fertilization (i.e. between 50 and 75 kg urea ha -1 ) as starter can be beneficial to improve growth, development, physiological traits and total yield of inoculated chickpea.