Major alkaloidal constituent from Impatiens niamniamensis seeds as antihyperglycemic agent

Abstract

Hypaphorine, an a-N,N,N-trimethyltryptophan betaine, is isolated as major constituent from Impatiens niamniamensis seeds (Balsaminaceae) for the first time. The structure of the compound was established by spectroscopic data. This is the first report of its in vivo evaluation for antihyperglycemic activity. The ethanolic extract of the seeds showed mild antihyperglycemic activity at the dose of 200 mg/kg body weight, whereas, hypaphorine showed significant activity in streptozotocin-induced diabetic rats at the dose of 50 mg/kg body weight when compared to standard drug metformin (100 mg/kg).

Hypaphorine, an Indole Alkaloid Isolated from Caragana korshinskii Kom., Inhibites 3T3‐L1 Adipocyte Differentiation and Improves Insulin Sensitivity in Vitro

“…3, treatment with different concentrations of hypaphorine (10 and 50 lM) remarkably inhibited cellular TG accumulation (P < 0.05 or 0.01) (Fig. [21] Effect of Hypaphorine on Expression of Adipocyte-Specific Genes during Adipocyte Differentiation Adipocyte differentiation from 3T3-L1 preadipocytes is associated with the expression of adipocyte-specific genes including PPARc and C/EBPa. 3d) in a dose-dependent manner.…”

“…Traditional Chinese medicine uses the roots, flowers, and seeds of C. korshinskii, for the treatment of high blood pressure, dizziness, menstruation, cancer, and other diseases. [20] A recent study indicated that hypaphorine showed significant antihyperglycemic activity in diabetic rats at the dose of 50 mg/kg body weight, [21] but the mechanism remains unknown. [13] [14] Hypaphorine was first isolated from seeds of C. korshinskii and has been detected in several other higher order plants.…”

“…However, efficient preparative separation and purification methods for natural compounds are needed. Due to the specific chemical structure and antihyperglycemic activity [21] of hypaphorine, we investigated its effect on differentiation and dexamethasone (DXM)-induced insulin resistance in 3T3-L1 cells. [22] [23] Considering the advantages of macroporous resin columns and semipreparative RPLC, these two techniques were combined in this study to isolate the target compound.…”

“…[17] [18] It is also known for its sleep promoting properties, stimulation of fermentation, [19] and antagonism of indole-3-acetic acid to finely regulate specific steps of plant growth or development. [20] A recent study indicated that hypaphorine showed significant antihyperglycemic activity in diabetic rats at the dose of 50 mg/kg body weight, [21] but the mechanism remains unknown.…”

“…1. Due to the specific chemical structure and antihyperglycemic activity [21] of hypaphorine, we investigated its effect on differentiation and dexamethasone (DXM)-induced insulin resistance in 3T3-L1 cells. The results indicated that hypaphorine inhibits adipocyte differentiation and improves insulin sensitivity in 3T3-L1 cells.…”