Major histocompatibility complex class I-restricted activation of cloned T cells by a soluble protein in the absence of accessory cells.

Dick, Thomas; Reske‐Kunz, Angelika B.

doi:10.1073/pnas.86.7.2316

Cited by 7 publications

(9 citation statements)

References 38 publications

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“…The Th1 cell clone BK-OVA-1R [7], which constitutively expresses IL-2R in low density, and the T-cell hybridoma 3DO-54.8 [17], both specific for OVA in combination with A b A k and I-A d , respectively, were established as described. BK-BI-2.6.O4.1 (BI/O4.1), an Ia-positive T-cell clone of (B106B10.BR) F1 origin, is able to present various protein Ag to Ag-specific CD4 + T cells [6]. A20/2 J is a mouse B-cell lymphoma derived from a BALB/c tumour [18].…”

Section: Methodsmentioning

confidence: 99%

“…Various cell types expressing class II molecules can act as APC, such as dendritic cells, B cells, lymphokine-activated macrophages and I-A transfected L-cells. We have reported on the Ag presentation capacity of the murine T-cell clone BK-BI-2.6.O4.1 (BI/O4.1) that constitutively synthesizes and expresses class II molecules [5,6]. The responding protein Ag-specific T clone cells were induced to produce lymphokines including IL-2.…”

Section: Introductionmentioning

confidence: 99%

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Augmented Antigen Presentation by Mouse Ia+ T Clone Cells BK‐BI–2.6.O4.1 Mediated by Transferrin Receptors

1996

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The murine T clone cells BK-BI-2.6.O4.1 (BI/O4.1) synthesize and express MHC class II molecules constitutively. BI/O4.1 cells are able to present various protein antigens to antigen-specific CD4 + T cells. However, a 10-fold higher concentration of antigen is needed to activate specific T cells to lymphokine secretion by BI/O4.1 cells in comparison with spleen cells or with the more homogeneous population of bone marrow-derived macrophages (BMMph). The authors tested whether the reduced antigen presentation potential of BI/O4.1 cells was augmented by transferrin-mediated uptake of the model antigen ovalbumin (OVA) coupled to human ferric transferrin. It was shown that 240-fold less OVA was sufficient to induce proliferation of an OVA-specific T-cell clone when the conjugate and not native OVA was used. The presence of ferric TF in the cultures competitively inhibited this effect of the conjugate. A similar shift in the dose-response curve to lower doses of antigen was induced by the conjugate when B lymphoma cells were used as antigen-presenting cells. BMMph and P388D1 cells processed and presented the conjugate with similar efficiency as native OVA, although both cell types exposed transferrin receptors. These data suggest that the reduced antigen presentation potential of BI/O4.1 T clone cells is due to the inefficient uptake of OVA by pinocytosis and delivery into the processing compartment.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Augmented Antigen Presentation by Mouse Ia+ T Clone Cells BK‐BI–2.6.O4.1 Mediated by Transferrin Receptors

1996

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“…A CD8 T and major histocompatibility complex (MHC) class I (HLAlymphocyte clone (TLC) can present cognate peptides to itself B27) -restricted CD8 ab TCR / cytotoxic T cells (CTL) [7] in the absence of other APC, and this mechanism of antigen with specificity for bacterial antigens are involved in the pathopresentation can induce proliferation [12] or death [13] of the genesis and maintenance of ReA. In the experiments reported, CTL.…”

Section: Rea Recognized and Efficiently Lysed Autologous And Allogenementioning

confidence: 99%

“…In the experiments reported, CTL. A CD8 TLC, 10BK.1, recognizes an exogenous soluble B cell lines and monocytes were used as professional antigenovalbumin-derived peptide together with MHC class I (H-2K b ) presenting cells (APC) in both proliferation and cytotoxicity molecules [12]. This clone has the capacity for self-presentation assays.…”

Section: Rea Recognized and Efficiently Lysed Autologous And Allogenementioning

confidence: 99%

Enterobacteria‐Infected T Cells as Antigen‐Presenting Cells for Cytotoxic CD8 T Cells: A Contribution to the Self‐Limitation of Cellular Immune Reactions in Reactive Arthritis?

et al. 1997

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In enterobacteria-induced reactive arthritis (ReA), different T cell subsets play a role in the induction and maintenance of the synovitic process. Synovial fluid-derived alphabeta CD4, alphabeta CD8, and gammadelta T lymphocyte clones (TLC) that recognize Yersinia or Salmonella antigens on professional antigen-presenting cells (APC) have been characterized, and T cells themselves can function as nonprofessional APC. T cells were infected with the facultatively intracellular, arthritogenic enterobacterium Yersinia enterocolitica O:3. A CD8 TLC isolated from a patient with Yersinia-induced ReA recognized and efficiently lysed autologous and allogeneic Yersinia-infected T cells. Infected cytotoxic T lymphocytes (CTL) had a reduced lytic capacity against syngeneic and allogeneic infected target cells, suggesting that the infection of CTL by bacteria may represent a mechanism of immune escape. In ReA, antigen presentation by T cells may modify the antibacterial immune response and may also contribute to network control mechanisms of T cell-mediated cytotoxicity.

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“…The consequences of such an inter-CTL antigen presentation can be lysis of the presenting CTL [14] as well as the stimulation of CTL to proliferation [3]. In previous studies we have shown that CTL clone 10BK.1 with specificity for the ovalbumin (OVA) peptide OVA 257 -264 proliferates after incubation with the soluble cognate peptide in the absence of added syngeneic APC, thus suggesting antigen self-presentation by the T cells [3,25]. In addition to the proliferative response, we observed lysis of syngeneic as well as allogeneic target cells after antigen self-presentation.…”

Section: Introductionmentioning

confidence: 99%

Proliferation and MHC-unrestricted bystander lysis by virus-specific cytotoxic T cells following antigen self-presentation

Staege¹,

Holtappels

Thomas

et al. 1998

Med Microbiol Immunol

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Cytotoxic T cells (CTL) not only act as effector cells, but can also serve as antigen-presenting cells (APC) for other CTL due to their expression of major histocompatibility complex (MHC) class I molecules. In the present study we show that independently derived CTL lines (CTLL) with specificity for an L(d)-presented nonapeptide corresponding to amino acids 168-176 of the immediate-early 1 (IE1) protein of murine cytomegalovirus not only lyse syngeneic but also allogeneic target cells, if the peptide is present during the cytolytic assay. Whereas a short peptide pulse is sufficient to render syngeneic cells susceptible to lysis, continued presence of soluble peptide is mandatory for the lysis of allogeneic target cells. This indicates a difference in the mechanisms involved. Syngeneic BALB/c B cell blasts (K(d)D(d)L(d)) and mutant BALB/c-H-2dm2 B cell blasts lacking the restricting Ld molecules (K(d)D(d)0) were lysed to a similar extent in the absence of the IE1 nonapeptide, provided that the IE1-specific CTL had been pre-incubated with the peptide before the cytolytic assay. Since the mutant cells cannot present the IE1 peptide, their lysis indicates an MHC-unrestricted, peptide-independent mode of recognition by the CTLL. In addition, proliferation of the CTLL takes place after incubation with the cognate peptide, even in the absence of professional APC. These data indicate inter-CTL antigen self-presentation, resulting in activation of the lytic machinery leading to peptide-independent bystander lysis of allogeneic as well as syngeneic target cells.

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Major histocompatibility complex class I-restricted activation of cloned T cells by a soluble protein in the absence of accessory cells.

Abstract: A T-cell clone, 10BK

Cited by 7 publications

References 38 publications

Augmented Antigen Presentation by Mouse Ia+ T Clone Cells BK‐BI–2.6.O4.1 Mediated by Transferrin Receptors

Augmented Antigen Presentation by Mouse Ia+ T Clone Cells BK‐BI–2.6.O4.1 Mediated by Transferrin Receptors

Enterobacteria‐Infected T Cells as Antigen‐Presenting Cells for Cytotoxic CD8 T Cells: A Contribution to the Self‐Limitation of Cellular Immune Reactions in Reactive Arthritis?

Proliferation and MHC-unrestricted bystander lysis by virus-specific cytotoxic T cells following antigen self-presentation

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