Major Histocompatibility Complex Class I and II Expression in Idiopathic Inflammatory Myopathy

Das, Leena; Blumbergs, Peter; Manavis, Jim; Limaye, Vidya

doi:10.1097/pai.0b013e31827d7f16

Cited by 37 publications

(36 citation statements)

References 23 publications

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“…In this study, we found a high specificity (0.891) and moderate sensitivity (0.605) for MHC-II expression in IIM and, as shown in Figure 5 found positive expression with varying sensitivities (25%-93%) or specificity (100%) in IIM [7,36]. MHC-II expression is necessary to activate T-helper cells and to initiate an immune response, and muscle cells can act as facultative antigen-presenting cells through the expression of MHC-II molecules [19,37].…”

Section: Text 14supporting

confidence: 62%

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An analysis of the sensitivity and specificity of MHC-I and MHC-II immunohistochemical staining in muscle biopsies for the diagnosis of inflammatory myopathies

Cruz

Luo

Miller

et al. 2014

Neuromuscular Disorders

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MURDOCH RESEARCH REPOSITORYThis is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.Abstract 1 Abstract 3 AbstractAlthough there have been several previous reports of immunohistochemical staining for MHC antigens in muscle biopsies, there appears to be a lack of consensus about its routine use in the diagnostic evaluation of biopsies from patients with suspected inflammatory myopathy. Positive MHC-I staining is nonspecific but is widely used as a marker for inflammatory myopathy, while the role of MHC-II staining is not clearly defined. We investigated the sensitivity and specificity of MHC-I and II immunostaining for the diagnosis of inflammatory myopathy in a large group of biopsies from a single reference laboratory. Positive staining for MHC-I was found to have a high sensitivity in biopsies from patients with inflammatory myopathy but a very low specificity, as it was also common in other non-inflammatory myopathies and neurogenic disorders.On the other hand, MHC-II positivity had a much higher specificity in all major subgroups of inflammatory myopathy, especially inclusion body myositis. The findings indicate that the combination of MHC-I and MHC-II staining results in a higher degree of specificity for the diagnosis of inflammatory myopathy and that in biopsies with inflammation, positive MHC-II staining strongly supports the diagnosis of an immunemediated myopathy. We recommend that immunohistochemical staining for both MHC-I and MHC-II should be included routinely in the diagnostic evaluation of muscle biopsies from patients with suspected inflammatory myopathy. However, as the sensitivity and interpretation of MHC staining may depend on the technique used, further studies are needed to compare procedures in different centres and develop standardised protocols.Abstract 4

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Section: Text 14supporting

confidence: 62%

“…A number of previous studies, which have been summarised in Tables 1 and 2, have reported positive immunohistochemical staining for MHC in IIM and other muscle conditions. Some studies have also addressed the diagnostic value of MHC expression with different methodologies and results [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

An analysis of the sensitivity and specificity of MHC-I and MHC-II immunohistochemical staining in muscle biopsies for the diagnosis of inflammatory myopathies

Cruz

Luo

Miller

et al. 2014

Neuromuscular Disorders

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“…MHC-II staining appears to be more specific, and is especially prominent in IBM [29] compared to the other IM. A recent report describes MHC-II staining to reach 100% in IBM tissues, while only 40% of NAM tissues were positive [30].…”

Section: Major Histocompatibility Complexesmentioning

confidence: 91%

Interferons as components of the complex web of reactions sustaining inflammation in idiopathic inflammatory myopathies

Paepe

2015

Cytokine

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“…The MHC class 1 expression on the sarcolemma, which is absent in normal muscle fibers, is upregulated in patients with IMs (29)(30)(31). Additionally, MHC class 2 is not expressed on normal myofibers, and some investigators have detected this antigen in specimens from patients with IMs (32)(33)(34)(35). HLA-DR was overexpressed on the sarcolemma in the PM group, but weakly expressed in the DM and CAM groups in the present study.…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Profile of Inflammatory Myopathy with Malignancy is Similar to that of Dermatomyositis rather than Polymyositis

et al. 2016

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Objective An association has been reported between inflammatory myopathies (IMs), which include polymyositis (PM) and dermatomyositis (DM), and malignancy, and the concept of cancer-associated myositis (CAM) was recently proposed. We herein attempted to determine the features and etiologies of these myopathies. Methods We analyzed the gene expression levels via microarray and real-time quantitative reverse transcription polymerase chain reaction analyses to identify genes that were specifically upregulated or downregulated with suspected inflammatory involvement and verified the microarray data via an immunohistochemical (IHC) analysis in additional cases. Patients We selected 14 patients with the following conditions: PM without malignancy (n=3), DM without malignancy (n=3), CAM (n=3), and Controls (no pathological changes or malignancy; n=5). Results PM was distinct from DM and CAM in a clustering analysis and exhibited the highest numbers of overexpressed genes and specific pathologies in a gene ontology analysis. The IHC analysis confirmed the gene expression results. Conclusion PM is associated with severe inflammatory pathological findings, primarily in the cell-mediated immune system. DM and CAM exhibit similarities in the gene expression and IHC results, which suggest that humoral immunity is the main etiology for both myopathies, indicating the importance of cancer screening in patients with IMs, particularly DM.

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Major Histocompatibility Complex Class I and II Expression in Idiopathic Inflammatory Myopathy

Abstract: Immunostaining for MHC I and II is a useful adjunctive test in diagnosis and subclassification of IIM.

Cited by 37 publications

References 23 publications

An analysis of the sensitivity and specificity of MHC-I and MHC-II immunohistochemical staining in muscle biopsies for the diagnosis of inflammatory myopathies

An analysis of the sensitivity and specificity of MHC-I and MHC-II immunohistochemical staining in muscle biopsies for the diagnosis of inflammatory myopathies

Interferons as components of the complex web of reactions sustaining inflammation in idiopathic inflammatory myopathies

Gene Expression Profile of Inflammatory Myopathy with Malignancy is Similar to that of Dermatomyositis rather than Polymyositis

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