Making Waves: Initiation and Propagation of Corticothalamic Ca2+ Waves In Vivo

Stroh, Albrecht; Adelsberger, Helmuth; Groh, Alexander; Rühlmann, Charlotta; Fischer, Sebastian; Schierloh, Anja; Deisseroth, Karl; Konnerth, Arthur

doi:10.1016/j.neuron.2013.01.031

Cited by 239 publications

(258 citation statements)

References 52 publications

Supporting

Mentioning

225

Contrasting

Order By: Relevance

“…2A, Fig. S3A, and Movie S2) (16,(21)(22)(23)(24)(25)(26). We found that such global brain activity (GBA) identified in CaS was mostly followed by brainwide activity in simultaneously recorded HemoS with a delay of a few seconds (Fig.…”

Section: Spontaneous Waves Of Cas Frequently Propagated Across the Wholementioning

confidence: 79%

“…To date, optical imaging and electrophysiological studies in animals have reported multiple heterogeneous dynamics of large-scale spontaneous neuronal activity (16,(21)(22)(23)(24)(25)(26)(27)(28). Waves of activity that propagate across the cortical network are commonly observed across different experimental setups (16,(21)(22)(23)(24)(25)(26).…”

mentioning

confidence: 99%

“…Waves of activity that propagate across the cortical network are commonly observed across different experimental setups (16,(21)(22)(23)(24)(25)(26). Recent fMRI studies by Mitra and coworkers (29)(30)(31) revealed that such large-scale propagation structure of spontaneous activity indeed has spatial information leading to Hemo-FC.…”

mentioning

confidence: 99%

“…In particular, it is important to clarify the relationship between two types of spontaneous neuronal activitynamely, the global waves (16,(21)(22)(23)(24)(25)(26) and the transient coactivations (28). One possibility is that the transient coactivations are caused by brief synchronization and desynchronization of neuronal activity in subsets of brain areas (34) and thus are distinct from the global waves.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Transient neuronal coactivations embedded in globally propagating waves underlie resting-state functional connectivity

Matsui

Murakami

Ohki

2016

Proc. Natl. Acad. Sci. U.S.A.

191

210

View full text Add to dashboard Cite

Resting-state functional connectivity (FC), which measures the correlation of spontaneous hemodynamic signals (HemoS) between brain areas, is widely used to study brain networks noninvasively. It is commonly assumed that spatial patterns of HemoSbased FC (Hemo-FC) reflect large-scale dynamics of underlying neuronal activity. To date, studies of spontaneous neuronal activity cataloged heterogeneous types of events ranging from waves of activity spanning the entire neocortex to flash-like activations of a set of anatomically connected cortical areas. However, it remains unclear how these various types of large-scale dynamics are interrelated. More importantly, whether each type of large-scale dynamics contributes to Hemo-FC has not been explored. Here, we addressed these questions by simultaneously monitoring neuronal calcium signals (CaS) and HemoS in the entire neocortex of mice at high spatiotemporal resolution. We found a significant relationship between two seemingly different types of large-scale spontaneous neuronal activity-namely, global waves propagating across the neocortex and transient coactivations among cortical areas sharing high FC. Different sets of cortical areas, sharing high FC within each set, were coactivated at different timings of the propagating global waves, suggesting that spatial information of cortical network characterized by FC was embedded in the phase of the global waves. Furthermore, we confirmed that such transient coactivations in CaS were indeed converted into spatially similar coactivations in HemoS and were necessary to sustain the spatial structure of Hemo-FC. These results explain how global waves of spontaneous neuronal activity propagating across large-scale cortical network contribute to Hemo-FC in the resting state.brain network | spontaneous activity | Ca imaging | optical imaging | fMRI

show abstract

Section: Spontaneous Waves Of Cas Frequently Propagated Across the Wholementioning

confidence: 79%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Transient neuronal coactivations embedded in globally propagating waves underlie resting-state functional connectivity

Matsui

Murakami

Ohki

2016

Proc. Natl. Acad. Sci. U.S.A.

191

210

View full text Add to dashboard Cite

show abstract

“…Specific features of the method are that it records strictly from the local clusters of cells that were labeled with the Ca 2+ indicator dye and that the recorded neuronal signals result exclusively from action potential activity, but not from subthreshold depolarization. The Ca 2+ signal detected by each fiber is collected from a local domain with a diameter of about 300-400 μm (9,10,24). The specific size of the domain depends on the dimensions of the cortical region that was stained with the Ca 2+ indicator, but also on the intensity of the excitation light and the collection efficiency of emitted fluorescence by the optical fiber.…”

Section: Discussionmentioning

confidence: 99%

Local domains of motor cortical activity revealed by fiber-optic calcium recordings in behaving nonhuman primates

Adelsberger

Zainos

Álvarez

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

Brain mapping experiments involving electrical microstimulation indicate that the primary motor cortex (M1) directly regulates muscle contraction and thereby controls specific movements. Possibly, M1 contains a small circuit "map" of the body that is formed by discrete local networks that code for specific movements. Alternatively, movements may be controlled by distributed, larger-scale overlapping circuits. Because of technical limitations, it remained unclear how movement-determining circuits are organized in M1. Here we introduce a method that allows the functional mapping of small local neuronal circuits in awake behaving nonhuman primates. For this purpose, we combined optic-fiber-based calcium recordings of neuronal activity and cortical microstimulation. The method requires targeted bulk loading of synthetic calcium indicators (e.g., OGB-1 AM) for the staining of neuronal microdomains. The tip of a thin (200 μm) optical fiber can detect the coherent activity of a small cluster of neurons, but is insensitive to the asynchronous activity of individual cells. By combining such optical recordings with microstimulation at two well-separated sites of M1, we demonstrate that local cortical activity was tightly associated with distinct and stereotypical simple movements. Increasing stimulation intensity increased both the amplitude of the movements and the level of neuronal activity. Importantly, the activity remained local, without invading the recording domain of the second optical fiber. Furthermore, there was clear response specificity at the two recording sites in a trained behavioral task. Thus, the results provide support for movement control in M1 by local neuronal clusters that are organized in discrete cortical domains. monkey | local circuits | neurophysiology | microendoscopy

show abstract

Isoflurane suppresses early cortical activity

Ситдикова

Захаров

Janáčková

et al. 2013

Ann Clin Transl Neurol

View full text Add to dashboard Cite

ObjectiveIsoflurane and other volatile anesthetics are widely used in children to induce deep and reversible coma, but they may also exert neurotoxic actions. The effects of volatile anesthetics on the immature brain activity remain elusive, however.MethodsThe effects of isoflurane on spontaneous and sensory-evoked activity were explored using intracortical extracellular field potential and multiple unit recordings in the rat barrel cortex from birth to adulthood.ResultsDuring the first postnatal week, isoflurane suppressed cortical activity in a concentration-dependent manner. At surgical anesthesia levels (1.5–2%), isoflurane completely suppressed the electroencephalogram and silenced cortical neurons. Although sensory potentials evoked by the principal whisker deflection persisted, sensory-evoked early gamma and spindle-burst oscillations were completely suppressed by isoflurane. Isoflurane-induced burst-suppression pattern emerged during the second postnatal week and matured through the first postnatal month. Bursts in adolescent and adult rats were characterized by activation of entire cortical columns with a leading firing of infragranular neurons, and were triggered by principal and adjacent whiskers stimulation, and by auditory and visual stimuli, indicating an involvement of horizontal connections in their generation and horizontal spread.InterpretationThe effects of isoflurane on cortical activity shift from total suppression of activity to burst-suppression pattern at the end of the first postnatal week. Developmental emergence of bursts likely involves a development of the intracortical short-and long-range connections. We hypothesize that complete suppression of cortical activity under isoflurane anesthesia during the first postnatal week may explain neuronal apoptosis stimulated by volatile anesthetics in the neonatal rats.

show abstract

Making Waves: Initiation and Propagation of Corticothalamic Ca2+ Waves In Vivo

Cited by 239 publications

References 52 publications

Transient neuronal coactivations embedded in globally propagating waves underlie resting-state functional connectivity

Transient neuronal coactivations embedded in globally propagating waves underlie resting-state functional connectivity

Local domains of motor cortical activity revealed by fiber-optic calcium recordings in behaving nonhuman primates

Isoflurane suppresses early cortical activity

Contact Info

Product

Resources

About