Malarial infection develops mitochondrial pathology and mitochondrial oxidative stress to promote hepatocyte apoptosis

Dey, Sumanta; Guha, Mithu; Alam, Athar; Goyal, Manish; Bindu, Samik; Pal, Chiranjib; Maity, Pallab; Mitra, Kalyan; Bandyopadhyay, Uday

doi:10.1016/j.freeradbiomed.2008.10.032

Cited by 74 publications

(53 citation statements)

References 72 publications

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“…Equal numbers of gastric mucosal primary cultured cells from control and experimental groups were used for the detection of intramitochondrial O 2 . using MitoSOX, a superoxide-sensitive fluorescence probe, following the protocol as described in the product catalogue (6,43). Cells were stained with the fluorescent probe in HBSS (pH 7.4) and incubated for 15 min at 37°C in the dark.…”

Section: Measurement Of Intramitochondrial Superoxide Anion (O 2 )mentioning

confidence: 99%

“…Tetraethoxypropane was used as a standard. Protein carbonyl was measured by following the standard colorimetric method that measures the binding of dinitrophenylhydrazine to the carbonyl group and was quantified by taking the absorbance at 362 nm (4,43).…”

Section: Measurement Of Intramitochondrial Free Iron In Gastric Mucosalmentioning

confidence: 99%

“…The fluorescence of each sample was measured in a Hitachi F-7000 fluorescence spectrofluorimeter (excitation 490 nm, emission 530 nm for JC-1 monomer; emission 590 nm for JC-1 aggregates). ⌬⌿ m was expressed as fluorescence ratio of 590 nm/530 nm (4,43).…”

Section: Assessment Of Mitochondrial Respiratory Function By Followinmentioning

confidence: 99%

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Tryptamine-Gallic Acid Hybrid Prevents Non-steroidal Anti-inflammatory Drug-induced Gastropathy

Pal

Dey

Alam

et al. 2012

Journal of Biological Chemistry

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Background: Non-steroidal anti-inflammatory drugs (NSAIDs) induce gastropathy by promoting mitochondrial pathology, oxidative stress, and apoptosis in gastric mucosal cells. Results: We have synthesized SEGA (3a), a tryptamine-gallic acid hybrid, which prevents NSAID-induced gastropathy by preventing mitochondrial oxidative stress, dysfunction, and apoptosis. Conclusion: SEGA (3a) bears an immense therapeutic potential against NSAID-induced gastropathy. Significance: This novel molecule is a significant addition in the discovery of gastroprotective drugs.

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Section: Measurement Of Intramitochondrial Superoxide Anion (O 2 )mentioning

confidence: 99%

Section: Measurement Of Intramitochondrial Free Iron In Gastric Mucosalmentioning

confidence: 99%

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Tryptamine-Gallic Acid Hybrid Prevents Non-steroidal Anti-inflammatory Drug-induced Gastropathy

Pal

Dey

Alam

et al. 2012

Journal of Biological Chemistry

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“…Elevated ⅐OH levels in P. yoelii-infected mice caused mitochondrial oxidative stress and hepatocyte apoptosis (63) and it is known that in vivo ⅐OH can directly damage nuclear DNA (64). It is also known that the glutathione (GSH) pathway via the thioredoxin redox system acts as a Plasmodium defense against oxidative damage such as ROS from the host immune system (65).…”

Section: Discussionmentioning

confidence: 99%

Sterile Protective Immunity to Malaria is Associated with a Panel of Novel P. falciparum Antigens

Trieu

Kayala

Burk

et al. 2011

Molecular & Cellular Proteomics

140

155

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The development of an effective malaria vaccine remains a global public health priority. Less than 0.5% of the Plasmodium falciparum genome has been assessed as potential vaccine targets and candidate vaccines have been based almost exclusively on single antigens. It is possible that the failure to develop a malaria vaccine despite decades of effort might be attributed to this historic focus. To advance malaria vaccine development, we have fabricated protein microarrays representing 23% of the entire P. falciparum proteome and have probed these arrays with plasma from subjects with sterile protection or no protection after experimental immunization with radiation attenuated P. falciparum sporozoites. A panel of 19 pre-erythrocytic stage antigens was identified as strongly associated with sporozoite-induced protective immunity; 16 of these antigens were novel and 85% have been independently identified in sporozoite and/or liver stage proteomic or transcriptomic data sets. Reactivity to any individual antigen did not correlate with protection but there was a highly significant difference in the cumulative signal intensity between protected and not protected individuals. Functional annotation indicates that most of these signature proteins are involved in cell cycle/DNA processing and protein synthesis. In addition, 21 novel blood-stage specific antigens were identified. Our data provide the first evidence that sterile protective immunity against malaria is directed against a panel of novel P. falciparum antigens rather than one antigen in isolation. These results have important implications for vaccine development, suggesting that an efficacious malaria vaccine should be multivalent and targeted at a select panel of key antigens, many of which have not been previously

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“…Mice were infected intraperitoneally with 1 ϫ 10 5 -1 ϫ 10 6 P. yoelii (multidrug-resistant strain)-parasitized red blood cells (RBCs). Infections were monitored by counting parasitized RBCs in thin smears of tail blood stained with Giemsa stain and expressed as percent parasitemia as described earlier (55)(56)(57).…”

Section: Methodsmentioning

confidence: 99%

Novel Anti-inflammatory Activity of Epoxyazadiradione against Macrophage Migration Inhibitory Factor

Alam

Haldar

Thulasiram

et al. 2012

Journal of Biological Chemistry

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Background: Macrophage migration inhibitory factor (MIF) is responsible for proinflammatory reactions in many infectious and non-infectious diseases. Results: Epoxyazadiradione, a limonoid, inhibits the tautomerase activity of both human and malarial MIF and prevents MIF-induced proinflammatory reactions. Conclusion: Epoxyazadiradione bears therapeutic potential against MIF-induced proinflammatory reactions. Significance: This novel molecule is a significant addition in the discovery of anti-inflammatory drugs.

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Malarial infection develops mitochondrial pathology and mitochondrial oxidative stress to promote hepatocyte apoptosis

Cited by 74 publications

References 72 publications

Tryptamine-Gallic Acid Hybrid Prevents Non-steroidal Anti-inflammatory Drug-induced Gastropathy

Tryptamine-Gallic Acid Hybrid Prevents Non-steroidal Anti-inflammatory Drug-induced Gastropathy

Sterile Protective Immunity to Malaria is Associated with a Panel of Novel P. falciparum Antigens

Novel Anti-inflammatory Activity of Epoxyazadiradione against Macrophage Migration Inhibitory Factor

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