Malathion Biodegradation by L. casei (NRRL1922) and L. acidophilus (NRRL 23431) in Fermented Skimmed Milk

Abstract

The aim of this study was to investigate and trace the biodegradation products of the pesticide malathion in a comparative manner by two different lactobacilli strains; L. casei (NRRL1922) and L. acidophilus (NRRL 23431). The two strains were cultivated separately into skimmed milk supplemented with 5 ng/ml malathion. After incubation under the appropriate conditions, randomized samples were taken at intervals 24, 48, 72 and 120 hours along with control samples and analyzed for the presence of malathion and its degradation products by the GC-MS spectrometry; As well as, analyzed to record the level of phosphatase enzyme which suggested to be involved in the biodegradation process. The results showed a high ability of the two tested strains to degrade malathion with a superiority of L. acidophilus (NRRL 23431) over L. casei (NRRL 1922). The level of phosphatase enzyme was elevated in both strains in the presence of malathion and decreased gradually upon the depletion of malathion from the sample, which reflects the role of the phosphatase enzyme in the biodegradation process.

One Health Probiotics as Biocontrol Agents: One Health Tomato Probiotics

“…Studies on tomato juice containing such bacteria have shown that this product can serve as a healthy drink for vegetarians or consumers who are allergic to dairy products [ 122 ]. The beneficial effects of the L. acidophilus [ 120 , 128 , 129 ], Lcb. casei and Lcb.…”

Biodegradation of Organophosphorus Pesticide (Malathion) by Bacillus sp. FYM31 Isolated from Agriculture Drainage Water

“…However, their excessive and inconsiderate usage affects the environment and becomes one of the factors of climate change [1, 2]. For pests , control, an organophosphorus insecticide has been extensively applied [3]. Malathion is considered one of the first and most commonly used OP and is still used extensively in Egypt, especially for agriculture [4].…”