MALDI–MS Profiling to Address Honey Bee Health Status under Bacterial Challenge through Computational Modeling

Arafah, Karim; Voisin, Sébastien; Masson, Victor; Alaux, Cédric; Conte, Yves Le; Bocquet, Michel; Bulet, Philippe

doi:10.1002/pmic.201900268

Cited by 15 publications

(15 citation statements)

References 57 publications

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“…Gut tissues were washed in PBS and then placed in low protein binding tubes (LoBind, Eppendorf, France) containing 20 μl of TFA 1% and vortexed. Hemolymph was collected from the same 12 bees according to the procedure published by Arafah and colleagues [69].…”

Section: Methodsmentioning

confidence: 99%

“…This approach which has been greatly improved over the past 20 years, has demonstrated its feasibility, robustness and cost-effectiveness in identifying a wide range of microorganisms (e.g., bacteria, mycobacteria and certain fungal pathogens [70], parasites [71,72] and more recently some microsporidia pathogenic for honeybee [73]. We also used MALDI MFPs to monitor the impact of experimental microbial infections in honeybee hemolymph [69]. To create model MFPs, an average spectrum for each tissue was recorded as the most representative histomolecular signature of the tissues (Figure S1).…”

Section: Tissue Signatures By Direct Maldi Profiling Experiments On H...mentioning

confidence: 99%

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Molecular histoproteomy by MALDI mass spectrometry imaging to uncover markers of the impact of Nosema on Apis mellifera

Houdelet

Arafah

Bocquet³

et al. 2022

Proteomics

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Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) is a powerful technology used to investigate the spatio-temporal distribution of a huge number of molecules throughout a body/tissue section. In this paper, we report the use of MALDI IMS to follow the molecular impact of an experimental infection of Apis mellifera with the microsporidia Nosema ceranae. We performed representative molecular mass fingerprints of selected tissues obtained by dissection. This was followed by MALDI IMS workflows optimization including specimen embedding and positioning as well as washing and matrix application. We recorded the local distribution of peptides/proteins within different tissues from experimentally infected versus non infected honeybees. As expected, a distinction in these molecular profiles between the two conditions was recorded from different anatomical sections of the gut tissue.More importantly, we observed differences in the molecular profiles in the brain, thoracic ganglia, hypopharyngeal glands, and hemolymph. We introduced MALDI IMS as an effective approach to monitor the impact of N. ceranae infection on A. mellifera. This opens perspectives for the discovery of molecular changes in peptides/proteins markers that could contribute to a better understanding of the impact of stressors and toxicity on different tissues of a bee in a single experiment.

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Section: Methodsmentioning

confidence: 99%

Section: Tissue Signatures By Direct Maldi Profiling Experiments On H...mentioning

confidence: 99%

Molecular histoproteomy by MALDI mass spectrometry imaging to uncover markers of the impact of Nosema on Apis mellifera

Houdelet

Arafah

Bocquet³

et al. 2022

Proteomics

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“…Among these results, the work of Arafah et al [ 231 ] proposed and developed MALDI–MS profiling to address honeybee health status under bacterial challenge through computational modeling, while Edwards and Dixon [ 232 ], in 2016 published a paper regarding negotiating the conflict between humans and honeybees towards an ecological city in the journal of Society and Animals .…”

Section: Nutritional Value Bioactive Components and Beneficial Properties Of Bee Productsmentioning

confidence: 99%

Bee Products: A Representation of Biodiversity, Sustainability, and Health

Durazzo¹,

Lucarini²,

Plutino

et al. 2021

Life

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Biodiversity strengthens the productivity of any ecosystem (agricultural land, forest, lake, etc.). The loss of biodiversity contributes to food and energy insecurity; increases vulnerability to natural disasters, such as floods or tropical storms; and decreases the quality of both life and health. Wild and managed bees play a key role in maintaining the biodiversity and in the recovery and restoration of degraded habitats. The novelty character of this perspective is to give an updated representation of bee products’ biodiversity, sustainability, and health relationship. The role of bees as bioindicators, their importance in the conservation of biodiversity, their ecosystem services, and the variety of the bee products are described herein. An overview of the main components of bee products, their biological potentials, and health is highlighted and detailed as follows: (i) nutritional value of bee products, (ii) bioactive profile of bee products and the related beneficial properties; (iii) focus on honey and health through a literature quantitative analysis, and (iv) bee products explored through databases. Moreover, as an example of the interconnection between health, biodiversity, and sustainability, a case study, namely the “Cellulose Park”, realized in Rome (Italy), is presented here. This case study highlights how bee activities can be used to assess and track changes in the quality of agricultural ecosystems—hive products could be valid indicators of the quality and health of the surrounding environment, as well as the changes induced by the biotic and abiotic factors that impact the sustainability of agricultural production and biodiversity conservation in peri-urban areas.

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“…Using modified OECD risk assessment protocols and fully crossed experiments, combined with MALDI BeeTyping ® and bottom-up proteomics by LC-ESI-MS/MS [ 22 , 63 , 64 ], we ask (i) how exposure to an insecticide, fungicide, and herbicide, individually or in combination with the parasite, impacts the haemolymph proteome profile; (ii) which proteins respond to these stressors; and (iii) how these responses map onto higher level effects of exposure on individual longevity, weight change, and parasite load.…”

Section: Introductionmentioning

confidence: 99%

Parasite and Pesticide Impacts on the Bumblebee (Bombus terrestris) Haemolymph Proteome

Askri

Straw

Arafah

et al. 2023

IJMS

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Pesticides pose a potential threat to bee health, especially in combination with other stressors, such as parasites. However, pesticide risk assessment tests pesticides in isolation from other stresses, i.e., on otherwise healthy bees. Through molecular analysis, the specific impacts of a pesticide or its interaction with another stressor can be elucidated. Molecular mass profiling by MALDI BeeTyping® was used on bee haemolymph to explore the signature of pesticidal and parasitic stressor impacts. This approach was complemented by bottom-up proteomics to investigate the modulation of the haemoproteome. We tested acute oral doses of three pesticides—glyphosate, Amistar and sulfoxaflor—on the bumblebee Bombus terrestris, alongside the gut parasite Crithidia bombi. We found no impact of any pesticide on parasite intensity and no impact of sulfoxaflor or glyphosate on survival or weight change. Amistar caused weight loss and 19–41% mortality. Haemoproteome analysis showed various protein dysregulations. The major pathways dysregulated were those involved in insect defences and immune responses, with Amistar having the strongest impact on these dysregulated pathways. Our results show that even when no response can be seen at a whole organism level, MALDI BeeTyping® can detect effects. Mass spectrometry analysis of bee haemolymph provides a pertinent tool to evaluate stressor impacts on bee health, even at the level of individuals.

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MALDI–MS Profiling to Address Honey Bee Health Status under Bacterial Challenge through Computational Modeling

Cited by 15 publications

References 57 publications

Molecular histoproteomy by MALDI mass spectrometry imaging to uncover markers of the impact of Nosema on Apis mellifera

Molecular histoproteomy by MALDI mass spectrometry imaging to uncover markers of the impact of Nosema on Apis mellifera

Bee Products: A Representation of Biodiversity, Sustainability, and Health

Parasite and Pesticide Impacts on the Bumblebee (Bombus terrestris) Haemolymph Proteome

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