MALDI-TOF MS and 16S RNA Identification of Culturable Gastric Microbiota: Variability Associated with the Presence of Helicobacter pylori

Pavez, Mónica; Cerda, Álvaro; Oporto, Marcelo; Villarroel, Daniel; Hofmann, E; Ríos, Eddy; Sierralta, Armando; Copelli, Luis; Barrientos, Leticia

doi:10.3390/microorganisms8111763

Cited by 9 publications

(6 citation statements)

References 55 publications

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“…Another diagnostic challenge is the correct identification on a species level. In some laboratories, matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) may not be as readily available as in others, even though this method has been considered the gold standard since its introduction into microbiological diagnostics more than a decade ago [ 48 , 49 , 50 ]. As described above, significant interspecies differences in clinical relevance, pathogenicity, and even antimicrobial susceptibility exist [ 50 , 51 ].…”

Section: The Diagnostic Complexities—how To Distinguish Between Infection and Contaminationmentioning

confidence: 99%

Update on Coagulase-Negative Staphylococci—What the Clinician Should Know

et al. 2021

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Coagulase-negative staphylococci (CoNS) are among the most frequently recovered bacteria in routine clinical care. Their incidence has steadily increased over the past decades in parallel to the advancement in medicine, especially in regard to the utilization of foreign body devices. Many new species have been described within the past years, while clinical information to most of those species is still sparse. In addition, interspecies differences that render some species more virulent than others have to be taken into account. The distinct populations in which CoNS infections play a prominent role are preterm neonates, patients with implanted medical devices, immunodeficient patients, and those with other relevant comorbidities. Due to the property of CoNS to colonize the human skin, contamination of blood cultures or other samples occurs frequently. Hence, the main diagnostic hurdle is to correctly identify the cases in which CoNS are causative agents rather than contaminants. However, neither phenotypic nor genetic tools have been able to provide a satisfying solution to this problem. Another dilemma of CoNS in clinical practice pertains to their extensive antimicrobial resistance profile, especially in healthcare settings. Therefore, true infections caused by CoNS most often necessitate the use of second-line antimicrobial drugs.

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Section: The Diagnostic Complexities—how To Distinguish Between Infection and Contaminationmentioning

confidence: 99%

Update on Coagulase-Negative Staphylococci—What the Clinician Should Know

et al. 2021

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“…It has been suggested that the presence of specific species such as Streptococcus mutans and Prevotella intermedia in the bio-film could inhibit the growth of H. pylori strains. [34]- [36] It also has been shown that Porphyromonas spp and Fusobacterium nucleatum can adhere to and trap H. pylori. [35] In addition to its effect on health, the incidence of Wolinella spp.…”

Section: Discussionmentioning

confidence: 99%

Identification and Prevalence of Helicobacter Pylori Virulence Genes Baba and Caga in Wolinella Isolates from the Oral Cavity of Dogs

Jahanshiri¹,

Nayeri-fasaei²,

Jamshidi³

et al. 2022

Preprint

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Wolinella spp. and Helicobacter spp. have been repeatedly reported in the oral cavity of dogs and are associated with periodontal disease. Wolinella strains predominate in the oral cavity of dogs. The only known species of this genus, Wolinella succinogenes, was considered non-pathogenic until sequence analysis of its genome revealed homologous genes resembling virulence factors in Helicobacter pylori. This has led researchers to question the nonpathogenic status of W. succinogenes. The cagA and babA genes are examples of crucial virulence factors in H. pylori pathogenesis; thus, the present study evaluated the prevalence of these genera and assessed the Wolinella strain genome in terms of the presence of these virulence factors. Multiple specific PCR tests were performed on oral secretion samples collected from 62 dogs by sterile cytobrush to evaluate the genera, species, and presence of virulence genes. The species-specific 16s rRNA genes from the Helicobacter and Wolinella genera were detected in 58.06% and 83.87% of the oral samples, respectively. H. pylori were not detected in the specimens. No cagA and babA genes were detected in the Wolinella spp. or non-pylori Helicobacter genomes. Our results confirmed that Wolinella spp. is the predominant population compared to Helicobacter in the oral cavity of dogs. Apparently, the incidence of Helicobacter infections is generally associated with non-pylori Helicobacter organisms. Despite the hypothesis of genomic homology between W. succinogenes and H. pylori, cagA and babA virulence genes were not identified in any of the oral samples from the dogs.

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“…Another interesting technique utilizing MALDI-TOF MS demonstrated a microbiota modification owing to H. pylori colonization linked to the gastric epithelial condition, implying a potential function for the microbiota in the development and progression of gastric disorders [ 82 ]. The presence of Neisseriaceae , Streptococcaceae , Enterobacteriaceae , and Lactobacillaceae families after H. pylori infection was seen, although Gram-positive bacteria such as Gamellaceae , Propionibacteriaceae , Granilucatella , Bacillaceae , and Corynebacterium species were removed.…”

Section: Matrix-assisted Laser Desorption/ionization–time-of-flight—m...mentioning

confidence: 99%

Evolution of Diagnostic Methods for Helicobacter pylori Infections: From Traditional Tests to High Technology, Advanced Sensitivity and Discrimination Tools

et al. 2022

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Rapid diagnosis and treatment application in the early stages of H. pylori infection plays an important part in inhibiting the transmission of this infection as this bacterium is involved in various gastric pathologies such as gastritis, gastro-duodenal ulcer, and even gastric neoplasia. This review is devoted to a quick overview of conventional and advanced detection techniques successfully applied to the detection of H. pylori in the context of a compelling need to upgrade the standards of the diagnostic methods which are currently being used. Selecting the best diagnostic method implies evaluating different features, the use of one or another test depending on accessibility, laboratories equipment, and the clinical conditions of patients. This paper aims to expose the diagnosis methods for H. pylori that are currently available, highlighting their assets and limitations. The perspectives and the advantages of nanotechnology along with the concept of nano(bio)sensors and the development of lab-on-chip devices as advanced tools for H. pylori detection, differentiation, and discrimination is also presented, by emphasizing multiple advantages: simple, fast, cost-effective, portable, miniaturized, small volume of samples required, highly sensitive, and selective. It is generally accepted that the development of intelligent sensors will completely revolutionize the acquisition procedure and medical decision in the framework of smart healthcare monitoring systems.

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MALDI-TOF MS and 16S RNA Identification of Culturable Gastric Microbiota: Variability Associated with the Presence of Helicobacter pylori

Cited by 9 publications

References 55 publications

Update on Coagulase-Negative Staphylococci—What the Clinician Should Know

Update on Coagulase-Negative Staphylococci—What the Clinician Should Know

Identification and Prevalence of <i>Helicobacter Pylori</i> Virulence Genes <i>Baba</i> and <i>Caga</i> in <i>Wolinella</i> Isolates from the Oral Cavity of Dogs

Evolution of Diagnostic Methods for Helicobacter pylori Infections: From Traditional Tests to High Technology, Advanced Sensitivity and Discrimination Tools

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