Malondialdehyde levels can be measured in serum and saliva by using a fast HPLC method with visible detection / Determinarea printr-o metodă HPLC-VIS rapidă a concentraţiilor serice şi salivare ale malondialdehidei

Fogarasi, Erzsébet; Croitoru, Mircea Dumitru; Fülöp, Ibolya; Nemes-Nagy, Enikő; Tripon, Robert Gabriel; Simon-Szabó, Zsuzsánna; Muntean, Daniela-Lucia

doi:10.1515/rrlm-2016-0029

Cited by 9 publications

(6 citation statements)

References 26 publications

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“…According to our data, the heart rate could represent such a clinical indicator, but clinicians should take into consideration other causes of inflammation and tissue damage that could lead to an increased fibrinogen level. Normalizing plasma fibrinogen level to total leukocyte or neutrophil counts could also be helpful in the clinical judgment, as well as measuring the level of biomarkers of oxidative stress [40][41][42][43][44].…”

Section: Discussionmentioning

confidence: 99%

Could fibrinogen and hsCRP be useful for assessing personal risk in workers exposed to a mixture of ultrafine particles and organic solvents?

Voidăzan

Moldovan

Huţanu

et al. 2018

Revista Romana De Medicina De Laborator

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7. IDEWE, External service for prevention and protection at work, Heverlee, Belgium AbstractPurpose: Our study focuses on elucidating if two common inflammatory biomarkers, easily performed in any laboratory -high-sensitivity C-reactive protein (hsCRP), as well as fibrinogen -could be used to assess the personal health risk of workers exposed to a complex occupational exposure to ultrafine particles (UFP) and a mixture of organic solvents. Methods: To assess the inflammatory response on the body, laboratory determinations were performed by testing the serum levels of hsCRP and fibrinogen, in exposed and unexposed groups. Results: There are no statistically significant differences for hsCRPs (p-0.25), medians were similar in groups. The mean values of fibrinogen in the three groups were: in the workers group (1st group): 346.2 mg/dl, in the office staff group (2nd group): 328.7 mg/dl, and in the control group (3rd group): 284.8 mg/dl, with significant differences between 1st group vs 3rd group and between 2nd group vs 3rd group (p-0.002). UFP levels differ between the groups, as follows: 1st group were exposed to the highest levels, ranging from 48349 to 3404000 part/cm3; 2nd group, ranging from 17371 to 40595 part/cm3; and 3rd group, ranging from 213 to 16255 part/cm3. Conclusions: Our study demonstrates that fibrinogen is a useful inflammatory biomarker for exposure to a mixture of UFP and organic solvents. On the other hand, hsCRP is not a useful inflammatory biomarker in occupational exposure to UFP and organic solvents. Further studies are needed to demonstrate the extent to which fibrinogen is more or less influenced by organic solvents or UFP alone.

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Section: Discussionmentioning

confidence: 99%

Could fibrinogen and hsCRP be useful for assessing personal risk in workers exposed to a mixture of ultrafine particles and organic solvents?

Voidăzan

Moldovan

Huţanu

et al. 2018

Revista Romana De Medicina De Laborator

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“…The degree of lipid peroxidation was determined by measuring brain and plasma MDA levels. MDA was determined by measuring thiobarbituric reactive species, combined with an HPLC method, according to the method that was previously reported by our group [ 55 ].…”

Section: Methodsmentioning

confidence: 99%

Drug Repurposing of Metformin for the Treatment of Haloperidol-Related Behavior Disorders and Oxidative Stress: A Preliminary Study

Jîtcă,

Gáll,

Jîtcă

et al. 2024

Pharmaceutics

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A particular attribute of the brain lies in the ability to learn, acquire information from the environment, and utilize the learned information. Previous research has noted that various factors (e.g., age, stress, anxiety, pathological issues), including antipsychotic medications, affect the brain and memory. The current study aimed to reveal the effects of chronic metformin treatment on the cognitive performance of rats and on commonly measured markers for oxidative stress. Wistar male rats (n = 40) were randomly divided into four groups: CTR (n = 10)–control group, METF (n = 10)–animals receiving metformin 500 mg/kg, HAL (n = 10)–animals receiving haloperidol 2 mg/kg, and HALMETF (n = 10)–animals receiving haloperidol 2 mg/kg and metformin 500 mg/kg. The medication was administered daily by oral gavage for 40 days. Memory and learning were assessed using the Morris Water Maze (MWM) test. At the end of the MWM, the rodents were decapitated under anesthesia, and the brain and blood samples were assayed by liquid chromatography for markers of oxidative stress (malondialdehyde, MDA, reduced/oxidized glutathione ratio, GSH/GSSG). The quantification of brain-derived neurotrophic factor (BDNF) was performed using the conventional sandwich ELISA technique. In the HALMETF group, metformin attenuated the negative effects of haloperidol. Brain and plasma MDA levels increased in the HAL group. Brain and plasma GSH/GSSG ratios and BDNF levels did not reveal any differences between groups. In conclusion, metformin treatment limits the deleterious cognitive effects of haloperidol. The effect on oxidative stress markers may also point toward an antioxidant-like effect of metformin, but this needs further tests for confirmation.

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“…values of gestational age at delivery were analyzed and proved high statistical significancepregnant women with PIH delivered a baby at earlier gestational age compared to healthy pregnant women [15]. The role of oxidative stress is stated as the cause of many diseases in numerous studies [12,[19][20][21]. In this research, medium values of TBARS were analyzed, as the oxidative stress marker, and compared with ultrasound parameters.…”

Section: Fig1 the Correlation Between Tbars And Cpr In Pregnant Women With Pih And Healthy Pregnant Woomenmentioning

confidence: 99%

“…As per the recommendation of the National High Blood Pressure Education Program Working Group on High Blood Pressure in Pregnancy, the categories are: chronic hypertension, preeclampsia-eclampsia, preeclampsia superimposed on chronic hypertension, gestational hypertension (transient hypertension of pregnancy or chronic hypertension identified in the latter half of pregnancy). This terminology is preferred to the older but widely used term PIH as it is more precise [2,3]. PIH is a multisystem disorder that involves several organ systems of the pregnant woman and fetus.…”

Section: Introductionmentioning

confidence: 99%

“…Free radicals can alter biomolecules, including the unsaturated fatty acids; deoxyribonucleic acid, ribonucleic acid. More recently, great attention has been paid to lipid peroxidation that involves the unsaturated fatty acids and generates several harmful products including malondialdehyde (MDA) [10][11][12]. MDA is the main product of lipid peroxidation and an indicator of oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

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The possibility of clinical use for the oxidative stress marker in correlation with blood flow parameters in pregnancy-induced hypertension

Draganović

Cancarevic-Djajic

Jojić³

et al. 2021

Revista Romana De Medicina De Laborator

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Introduction: In pregnancy-induced hypertension (PIH) there is a disorder in placental blood flow which causes intrauterine fetal hypoxia, and oxidative stress has a significant role in this condition. The aims of this research were to analyze the relation of thiobarbituric acid reactive substance (TBARS), as a marker of oxidative stress and absent end-diastolic flow (AEDF), as well as the relation of TBARS and cerebroplacental ratio (CPR). Methods: The research included 200 pregnant women in the gestation period from 28th to 40th, 100 were in a control group and 100 were with PIH. The CPR and TBARS were analyzed in all examined pregnant women. The CPR was calculated by dividing the Doppler indices of the middle cerebral artery by the umbilical artery. Results: Mean value of TBARS in the group with the PIH who had AEDF was in the interval of high values - 43.22 μmol/l. The result shows that through the application of Spearman’s coefficient, the correlation results in a statistically significant correlation between CPR and TBARS values: ρ = - 0.249, p = 0.0001. Conclusion: PIH has a very high level of oxidative stress, especially in pregnant women with absent end-diastolic flow in the umbilical artery and pathologic CPR. The Spearman’s test results in statistical significance and negative correlation, which means that in higher TBARS values, values of CPR are lower and vice versa, which indicates a possibility of clinical application of TBARS.

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Malondialdehyde levels can be measured in serum and saliva by using a fast HPLC method with visible detection / Determinarea printr-o metodă HPLC-VIS rapidă a concentraţiilor serice şi salivare ale malondialdehidei

Cited by 9 publications

References 26 publications

Could fibrinogen and hsCRP be useful for assessing personal risk in workers exposed to a mixture of ultrafine particles and organic solvents?

Could fibrinogen and hsCRP be useful for assessing personal risk in workers exposed to a mixture of ultrafine particles and organic solvents?

Drug Repurposing of Metformin for the Treatment of Haloperidol-Related Behavior Disorders and Oxidative Stress: A Preliminary Study

The possibility of clinical use for the oxidative stress marker in correlation with blood flow parameters in pregnancy-induced hypertension

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