Malt sprout extract medium for cultivation of Lactobacillus plantarum protective cultures

Abstract

Aims:The aim was to develop a cheap cereal-based alternative medium for the large-scale production of biopreservative Lactobacillus plantarum VTT E-79098. We examined the effect of growth medium and pH control on the cell yield of Lact. plantarum E-79098 and the antimicrobial activity of the cell-free extracts. Methods: Fermentations using a novel Malt Sprout Extract Medium (MSE) were performed with different pH regimes. The antimicrobial activity of the cell-free extracts against Pantoea agglomerans VTT E-90396 and Fusarium avenaceum VTT D-80147 was assessed with automated turbidometry. Significance and Impact of the Study: When compared with MRS, the MSE medium cultures produced equal growth yields of Lact. plantarum VTT E-79098 and enhanced antimicrobial potential against the Gram-negative bacterium P. agglomerans and a Fusarium fungus. The MSE medium can be used as a low-cost alternative to MRS for producing high cell yields and good antimicrobial activity of Lact. plantarum.

Optimization of probiotic and lactic acid production by Lactobacillus plantarum in submerged bioreactor systems

“…Yeast extract concentration and stirred agitation, which showed no significant influence on cell production, were set at 5 g l -1 and 200 rpm, respectively. Laitila et al [16] also observed that increased concentration of yeast extract from 4 to 8 g l -1 did not improve cell growth.…”

“…Temperature and pH negatively influenced biomass production. The maximal viable cell count of L. plantarum was obtained with MSEextra medium in bioreactor fermentation when pH was controlled at 5.0 [16], which is near the lowest level used in the PB design (5.2).…”

A survey of some antifungal properties of lactic acid bacteria isolates from koumiss in China

“…Recent studies reported that Lactobacillus could inhibit the growth of Fusarium (Laitila et al. 2004; Hassan and Bullerman 2008).…”

Production of a potentially probiotic culture of Lactobacillus casei subsp. casei CECT 4043 in whey

“…These observations suggest that the antibacterial activity contained in the Lb. casei CECT 4043 culture supernatants could be the result of the synergistic action of two or more inhibitory compounds produced by this strain (Guerra & Pastrana, 2003;Laitila et al, 2004;Niku-Paavola, Laitila, MattilaSandholm, & Haikara, 1999). Since lactic acid was the unique organic acid detected in the culture medium, antibacterial activity also could be related to the production of bacteriocins by Lb.…”

“…In small scale studies, LAB is commonly cultured in commercial laboratory media (e.g., MRS and tryptone glucose extract (TGE) broth), but for large scale production the use of these media can be cost-prohibitive (Laitila et al, 2004). In these cases, high-cost complex culture media must be replaced with low-cost media based on by-products of the food industry (Doleyres & Lacroix, 2005;Guerra & Pastrana, 2003;Guerra, Fajardo, Torrado, Ló pez, & Pastrana, 2005;.…”