Maltoheptaose promotes nanoparticle internalization by Escherichia coli

Jayawardena, H. Surangi N.; Jayawardana, Kalana W.; Chen, Xuan; Yan, Mingdi

doi:10.1039/c3cc40491a

Cited by 53 publications

(64 citation statements)

References 38 publications

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“…7) [45,46]. On irradiation with UV light, the azide group is transformed into a highly reactive nitrene functionality that can insert into CH and NH bonds.…”

Section: Covalent Functionalization With Carbohydratesmentioning

confidence: 99%

Recent advances in biosensing using magnetic glyconanoparticles

2015

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In this critical review we discuss the most recent advances in the field of biosensing applications of magnetic glyconanoparticles. We first give an overview of the main synthetic routes to obtain magnetic-nanoparticle-carbohydrate conjugates and then we highlight their most promising applications for magnetic relaxation switching sensing, cell and pathogen detection, cell targeting and magnetic resonance imaging. We end with a critical perspective of the field, identifying the main challenges to be overcome, but also the areas where the most promising developments are likely to happen in the coming decades.

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“…7) [45,46]. On irradiation with UV light, the azide group is transformed into a highly reactive nitrene functionality that can insert into CH and NH bonds.…”

Section: Covalent Functionalization With Carbohydratesmentioning

confidence: 99%

Recent advances in biosensing using magnetic glyconanoparticles

2015

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“…Bacterial imaging has thus been demonstrated in several examples, either using the optical properties of the nanomaterial cores themselves, such as quantum dots and crosslinked polydiacetylene, [56,87,101] or by introducing fluorescent labels in the materials. [42,85,94,96] Furthermore, magnetic nanoparticles enable the use of magnetic resonance imaging.…”

Section: Glyconanomaterialsmentioning

confidence: 99%

“…[115–118] Uptake of functionalized nanoparticles of core sizes <6 nm have also been proposed. [87,119,120] For example, Nadeau and coworkers reported that adenine- and AMP-conjugated CdSe and CdSe/ZnS quantum dots were internalized in B. subtilis and E. coli , presumably through purine-dependent mechanisms. [120] Furthermore, Yan and coworkers showed that maltoheptaose-functionalized magnetic iron oxide nanoparticles appeared to be internalized in E. coli , also suggesting that translocation through the inner membrane is a major barrier.…”

Section: Glyconanomaterialsmentioning

confidence: 99%

Glyconanomaterials for Combating Bacterial Infections

Ramström

Yan

2015

Chemistry A European J

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Bacterial infections constitute an increasing problem to human health in response to build-up of resistance to present antibiotics and sluggish development of new pharmaceuticals. However, a means to address this problem is to pinpoint the drug delivery to - and into - the bacteria. This results in a high local concentration of the drug, circumventing the increasingly high doses otherwise necessary. Combined with other effectors, such as covalent attachment to carriers, rendering the drugs less degradable, and the combination with efflux inhibitors, old drugs can be revived. In this context, glyconanomaterials offer exceptional potential, since these materials can be tailored to accommodate different effectors. In this feature article, we describe the different advantages of glyconanomaterials, and point to their importance in antibiotic ‘revitalization’.

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“…The plates were incubated at 37°C for 16-18 h. A resazurin solution (10 or 20 μL of 0.01 w/vol % to wells with 100 or 200 μL drug/bacteria mixture, respectively) was added to each sample, and the plates were incubated for 2 more hours, whereafter the color change was noted. The MICs obtained by color change corresponded to those from turbidity measurement (47), except where free drug precipitated. For extrapolation of partial growth inhibition by Gompertz function (48), the turbidity was recorded.…”

Section: -90 25mentioning

confidence: 99%

“…MIC Assays. MIC was determined by broth microdilution (46,47), with slight modifications. Liquid cultures of bacteria (E. coli ATCC 25922, E. coli ORN 178, E. coli ORN 208) were grown in log phase and diluted to 10 6 cfu/mL The aqueous nanoaggregates suspension was diluted sequentially with water.…”

Section: -90 25mentioning

confidence: 99%

Design and synthesis of theranostic antibiotic nanodrugs that display enhanced antibacterial activity and luminescence

Xie

Manuguri

Proietti

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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We report the modular formulation of ciprofloxacin-based pure theranostic nanodrugs that display enhanced antibacterial activities, as well as aggregation-induced emission (AIE) enhancement that was successfully used to image bacteria. The drug derivatives, consisting of ciprofloxacin, a perfluoroaryl ring, and a phenyl ring linked by an amidine bond, were efficiently synthesized by a straightforward protocol from a perfluoroaryl azide, ciprofloxacin, and an aldehyde in acetone at room temperature. These compounds are propeller-shaped, and upon precipitation into water, readily assembled into stable nanoaggregates that transformed ciprofloxacin derivatives into AIE-active luminogens. The nanoaggregates displayed increased luminescence and were successfully used to image bacteria. In addition, these nanodrugs showed enhanced antibacterial activities, lowering the minimum inhibitory concentration (MIC) by more than one order of magnitude against both sensitive and resistant Escherichia coli. The study represents a strategy in the design and development of pure theranostic nanodrugs for combating drug-resistant bacterial infections.nanodrugs | aggregation-induced emission | fluoroquinolones

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Maltoheptaose promotes nanoparticle internalization by Escherichia coli

Abstract: Nanoparticles conjugated with D-maltoheptaose (G7) showed a striking increase in the internalization by Escherichia coli. This applies to strains with and without the maltodextrin transport channel and particles ranging from a few to a hundred nanometers.

Cited by 53 publications

References 38 publications

Recent advances in biosensing using magnetic glyconanoparticles

Recent advances in biosensing using magnetic glyconanoparticles

Glyconanomaterials for Combating Bacterial Infections

Design and synthesis of theranostic antibiotic nanodrugs that display enhanced antibacterial activity and luminescence

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