Mammalian Cell Culture Reactors, Scale‐Up

Griffiths, J. B.

doi:10.1002/9780470054581.eib396

Cited by 5 publications

(9 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The upstream investment cost of the STR option is 1.7 times that of the HFB option, but the direct operating costs of the HFB option were higher because of the disposable HF cartridges, and as a result, the upstream total operating costs are almost equal (STR 762 k€ and HFB 787 k€). The result is in agreement with the general opinion, that in scale up to several grams, the HFB is a viable and economical alternative 33, 47–49…”

Section: Resultssupporting

confidence: 91%

“…Most hybridoma cell lines are grown in air‐lift or in HFBs 9, 32. The hollow fiber (HF) system gives a very large surface area for attaching cells and allows continuous removal of waste products and supply of nutrients 33…”

Section: Production Datamentioning

confidence: 99%

“…The HF cartridge consists of a series of capillary tubes, and the cells grow in the extracapillary space (ECS) and fresh medium is circulated through the tubes, that is in the intracapillary space. Ultrafiltration (UF) membrane is used frequently as a fiber material 33, 34…”

Section: Production Datamentioning

confidence: 99%

See 2 more Smart Citations

Economic comparison of diagnostic antibody production in perfusion stirred tank and in hollow fiber bioreactor processes

Vermasvuori

Hurme

2011

Biotechnology Progress

View full text Add to dashboard Cite

The total operating costs of small-scale monoclonal antibody production were calculated for two different upstream options and general downstream procedure based on protein A chromatography. The upstream options were a spin-filter equipped stirred-tank bioreactor (STR) and a hollow fiber bioreactor (HFB). Both the bioreactors were operated in perfusion mode. The total operating costs of the processes were 6,900 €/g for STR option and 6,400 €/g for the HFB option. In the both systems, the costs were dominated by expenses derived from the downstream section (almost 80%) that was almost identical in the both systems. In the upstream section, the investment depreciation was the largest cost item. The lower total costs of the HFB option were a result of lower investment costs and more concentrated product that led into savings also in downstream section. This study brings out the HFB as on viable alternative for stirred-tank bioreactor, especially in small-scale diagnostic monoclonal antibody production.

show abstract

Section: Resultssupporting

confidence: 91%

Section: Production Datamentioning

confidence: 99%

See 1 more Smart Citation

Economic comparison of diagnostic antibody production in perfusion stirred tank and in hollow fiber bioreactor processes

Vermasvuori

Hurme

2011

Biotechnology Progress

View full text Add to dashboard Cite

show abstract

“…The biological systems, BioS ( The BioS in Figure 5A show biological functions that to large extent can be described by established kinetic rate equations for the biological components involved [1,3], e.g. the uptake of nutrient substrates:…”

Section: Figurementioning

confidence: 99%

“…Increasing industrial use of mammalian cell cultures for a variety of purposes, such as production of biopharmaceuticals [3][4][5] and expansion and differentiation of embryonic stems [6][7][8][9], emphasizes the need of reliable and successful scale-up methodology.…”

Section: Introductionmentioning

confidence: 99%

Scale‐up of cell culture bioreactors using biomechatronic design

Mandenius

Björkman

2012

Biotechnology Journal

View full text Add to dashboard Cite

An alternative approach for scale-up of cell culture bioreactors based on biomechatronic design methodology is suggested.The approach differs from traditional biochemical engineering methodology by applying a sequential design procedure where the needs of the users and alternative design solutions based on the biological and technical functions of the scaled-up bioreactor are derived in functional maps, concept generation charts and scoring and interaction matrices. Basic reactor engineering properties, such mass and heat transfer and kinetics are integrated in the procedure. Examples are provided from production of monoclonal antibody and recombinant protein.

show abstract

Substrate Optimization in Baby Hamster Kidney Cell Culture for Foot and Mouth Disease Virus Vaccine Using the Taguchi Method

et al. 2020

View full text Add to dashboard Cite

Cell culture is one of the most commonly used techniques in the production of biological products. Many physical and chemical parameters may affect cell growth and proliferation. This study was conducted to investigate the effect of chemical components as supplements using the experimental design method, which aimed at reducing the number of experiments. For this purpose, supplements including chemical components using four levels, with three replications in suspension and batch culture conditions, were examined for 72 hours using the Taguchi experimental design method. From the experiments, it was concluded that the culture media composition had a significant impact on final cell count and pH. High concentrations of different media composition alone were insufficient to ensure higher cell count. According to the results, this insufficiency was associated with an increase of 20% in the number of final cells. In the majority of cultures, the number of final cells at 48 hours increased relative to the number of final cells at 24 hours after culturing the cells.

show abstract

Mammalian Cell Culture Reactors, Scale‐Up

Cited by 5 publications

References 77 publications

Economic comparison of diagnostic antibody production in perfusion stirred tank and in hollow fiber bioreactor processes

Economic comparison of diagnostic antibody production in perfusion stirred tank and in hollow fiber bioreactor processes

Scale‐up of cell culture bioreactors using biomechatronic design

Substrate Optimization in Baby Hamster Kidney Cell Culture for Foot and Mouth Disease Virus Vaccine Using the Taguchi Method

Contact Info

Product

Resources

About